A5081422673- Photoacoustic and Ultrasonic Imaging
- Nanoplatforms for cancer theranostics
- Optical Imaging and Spectroscopy Techniques
- Extracellular vesicles in disease
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Peptidase Inhibition and Analysis
- Electron Spin Resonance Studies
- Thermography and Photoacoustic Techniques
- Estrogen and related hormone effects
- Inflammatory mediators and NSAID effects
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Photodynamic Therapy Research Studies
- Infectious Diseases and Mycology
- Conducting polymers and applications
- Tryptophan and brain disorders
- Urological Disorders and Treatments
- Alzheimer's disease research and treatments
- Hernia repair and management
- Neuroinflammation and Neurodegeneration Mechanisms
- Redox biology and oxidative stress
- Systemic Lupus Erythematosus Research
- Medical Imaging Techniques and Applications
- Adipose Tissue and Metabolism
- Nanoparticle-Based Drug Delivery
University of Tübingen
2019-2024
Siemens (Germany)
2018-2024
University Children's Hospital Tübingen
2022
Imaging Center
2019
Technical University of Munich
2011-2018
Helmholtz Zentrum München
2011-2018
Laboratoire d’Imagerie Biomédicale
2014
Centre National de la Recherche Scientifique
2010-2013
Université Paris Cité
2010-2013
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
2013
A functional cancer theranostic nanoplatform is developed, specifically tailored toward the optoacoustic modality by combining gold nanorods with DNA nanostructures (D–AuNR). origami used as an efficient delivery vehicle owing to its prominent tumor-targeting property. The D–AuNR hybrids display enhanced tumor diagnostic sensitivity improved imaging and excellent photothermal therapeutic properties in vivo. As a service our authors readers, this journal provides supporting information...
To investigate whether multispectral optoacoustic tomography (MSOT) can reveal the heterogeneous distributions of exogenous agents interest and vascular characteristics through tumors several millimeters in diameter vivo.Procedures involving animals were approved by government Upper Bavaria. Imaging subcutaneous mice was performed using an experimental MSOT setup that produces transverse images at 10 frames per second with in-plane resolution approximately 150 μm. study dynamic contrast...
Abstract Early detection of cancer greatly increases the chances a simpler and more effective treatment. Traditional imaging techniques are often limited by shallow penetration, low sensitivity, specificity, poor spatial resolution or use ionizing radiation. Hybrid modalities, like optoacoustic imaging, an emerging molecular modality, contribute to improving most these limitations. However, this method is hindered relatively signal contrast. Here, gold nanoprisms (AuNPrs) used as amplifiers...
Abstract Difficulty in visualizing glioma margins intraoperatively remains a major issue the achievement of gross total tumor resection and, thus, better clinical outcome glioblastoma (GBM) patients. Here, potential new combined optical + optoacoustic imaging method for intraoperative brain delineation is investigated. A strategy using newly developed gold nanostar synthesis method, Raman reporter chemistry, and silication to produce dual‐modality contrast agents surface‐enhanced resonance...
The design of liposome-nanoparticle hybrids offers a rich toolbox for the fabrication multifunctional modalities. A self-assembled liposome-gold nanorod hybrid vesicular system that consists lipid-bilayer-associated gold nanorods designed to allow deep tissue detection, therapy, and monitoring in living animals using multispectral optoacoustic tomography has been fabricated characterized vitro vivo.
We have synthesized a targeted imaging agent for rheumatoid arthritis based on polysulfated gold nanorods.The CTAB layer nanorods was first replaced with PEG-thiol and then dendritic polyglycerolsulfate at elevated temperature, which resulted in significantly reduced cytotoxicity compared to polyanionic functionalized by non-covalent approaches.In addition classical characterization methods, we established facile UV-VIS BaCl 2 agglomeration assay confirm quantitative removal of unbound...
Abstract Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease of immunocompromised humans, caused by the opportunistic fungal pathogen Aspergillus fumigatus . Inadequacies in current diagnostic procedures mean that early diagnosis disease, critical to patient survival, remains major clinical challenge, and leading empiric use antifungal drugs emergence azole resistance. A non-invasive procedure allows both unambiguous detection IPA its response treatment therefore...
To investigate the feasibility of a high resolution optical imaging strategy for myocardial infarction.Near-infrared approaches to cardiovascular disease enable visualization disease-associated biological processes in vivo. However, even at scale small animals, strong scattering light prevents after first 1-2 mm tissue, leading degraded signal localization.Multispectral optoacoustic tomography (MSOT) was used non-invasively image infarction (MI) murine model coronary artery ligation...
Abstract Efficient delivery of short interfering RNAs reflects a prerequisite for the development RNA interference therapeutics. Here, we describe highly specific nanoparticles, based on near infrared fluorescent polymethine dye-derived targeting moieties coupled to biodegradable polymers. The dye, even when nanoparticle, mimics ligand hepatic parenchymal uptake transporters resulting in hepatobiliary clearance approximately 95% dye within 45 min. Body distribution, hepatocyte and excretion...
Objective Rheumatoid arthritis (RA) is one of the most frequent inflammatory diseases, causing pain and disability in affected joints. Early diagnosis essential for efficiency symptom‐targeting treatments, but its requires careful clinical, serologic, imaging examinations, such as magnetic resonance (MRI), which both expensive time consuming. In an effort to provide biomedical community with a more accessible way assess advancement arthritis, this study sought investigate use multispectral...
The International Atomic Energy Agency organized a technical meeting at its headquarters in Vienna, Austria, 2022 that included 17 experts representing 12 countries, whose research spanned the development and use of radiolabeled agents for imaging infection. focused largely on bacterial pathogens. group discussed evaluated advantages disadvantages several radiopharmaceuticals, as well science driving various approaches. main objective was to understand why few infection-targeted radiotracers...
The leading cause of stress urinary incontinence in women is the urethral sphincter muscle deficiency caused by mechanical during pregnancy and vaginal delivery. In men, prostate cancer surgery injury local nerves muscles are associated with incontinence. Current treatment often fails to satisfy patient's needs. Cell therapy may improve situation. We therefore investigated regeneration potential cells ameliorating a large animal model. Urethral was induced surgically gilts electrocautery...
This study describes the simultaneous <italic>in vivo</italic> optoacousic imaging and siRNA-mediated gene silencing capabilities of a model theranostic vector system between liposomes gold nanorods.
We investigated in vitro and vivo the optoacoustic responses of silicon 2,3-naphthalocyanine bi(trihexylsilyloxide) (SiNc), considered herein as a reporter molecule for imaging, elucidating its efficiency (photoacoustic) signal generation examining performance achieved. <b>Methods:</b> SiNc solutions were prepared using Cremophor EL water evaluated light-absorbing contrast-generating properties. Photostability singlet oxygen under pulsed laser illumination validated photoabsorbance. HT-29...
Diversity of the design and alignment illumination ultrasonic transducers empower fine scalability versatility optoacoustic imaging. In this study, we implement an innovative high-resolution mesoscopy for imaging vasculature tissue oxygenation within subcutaneous orthotopic cancerous implants mice in vivo through acquisition tomographic projections over 180° at a central frequency 24 MHz. High-resolution volumetric was combined with multispectral functional measurements to resolve exquisite...
Invariably fatal and with a particularly fast progression, pulmonary fibrosis (PF) is currently devoid of curative treatment options. Routine clinical diagnosis relies on breathing tests visualizing the changes in lung structure by CT, but anatomic information often not sufficient to identify early signs progressive PF. For more efficient diagnosis, additional imaging techniques were investigated combination such as 18F-FDG PET, although limited success because lack disease specificity....
The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic (MSOT) imaging technologies, was investigated. Viability induction apoptosis DiR MSCs were assessed by XTT- Caspase-3/-7-testing in vitro. 2 × 106, 105 104 5 10 μg DiR/ml into fresh frozen hearts. FMT-XCT, MSOT...
A strategy is described that allows the easy assembly and controlled disassembly of drug conjugates. Imide ligation, is, reaction a peptide thioacid with an azidoformate, used for conjugate assembly. The imide bond participates also endopeptidase-triggered cyclization-based mechanism.
Background: Traditional diagnosis is based on histology or clinical-stage classification which provides no information tumor metabolism and inflammation, which, however, are both hallmarks of cancer directly associated with prognosis severity. This project was an exploratory approach to profile metabolites, lipoproteins, inflammation parameters (glycoprotein A glycoprotein B) borderline ovarian (BOT) high-grade serous (HGSOC) for identifying additional useful serum markers stratifying...