A5007069527- Metabolomics and Mass Spectrometry Studies
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Porphyrin and Phthalocyanine Chemistry
- Tryptophan and brain disorders
- Diet and metabolism studies
- Crystallization and Solubility Studies
- Viral Infections and Immunology Research
- X-ray Diffraction in Crystallography
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Alzheimer's disease research and treatments
- Diabetes and associated disorders
- Liver Disease Diagnosis and Treatment
- Magnetism in coordination complexes
- interferon and immune responses
- Hepatitis C virus research
- Stroke Rehabilitation and Recovery
- Thermal Regulation in Medicine
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Infectious Encephalopathies and Encephalitis
- Surface Chemistry and Catalysis
Siemens (Germany)
2020-2025
University of Tübingen
2020-2025
STZ eyetrial
2023-2024
Imaging Center
2023
NS Kurnakova Institute of General and Inorganic Chemistry
2020-2021
Background Deep metabolomic, proteomic and immunologic phenotyping of patients suffering from an infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have matched a wide diversity clinical symptoms potential biomarkers for disease 2019 (COVID-19). Several studies described the role small as well complex molecules such metabolites, cytokines, chemokines lipoproteins during in recovered patients. In fact, after SARS-CoV-2 viral almost 10-20% experience persistent post 12...
Background Beta-amyloid (Abeta) and tau protein in cerebrospinal fluid (CSF) are established diagnostic biomarkers for Alzheimer’s disease (AD). However, these may not the only ones existing parameters that reflect neuropathological change. The use of quantitative metabolomics approach could provide novel insights into dementia progression identify key metabolic alterations CSF serum. Methods In present study, we quantified a set 45 metabolites (71 patients) 27 serum (76 patients with mild...
Abstract Background Breast cancer is a metabolically heterogeneous disease, and although the concept of metabolism known, its precise role in human breast yet to be fully elucidated. Methods We investigated an explorative approach cohort 42 primary mamma carcinoma patients with positron emission tomography/magnetic resonance imaging (PET/MR) prior surgery, followed by histopathology molecular diagnosis. From subset patients, which showed high metabolic heterogeneity based on tracer uptake...
Alzheimer's disease (AD) is the most common form of dementia in elderly and has been associated with changes lipoprotein metabolism. We performed quantitative analysis a local cohort cognitively impaired control subjects using standardized nuclear magnetic resonance (NMR) spectroscopy. A commercially available NMR-based assay covering 112 main subtype variables was used to investigate blood serum samples from moderate size 161 persons (71 female, 90 male), including measures quality control....
Abstract Background Diagnostic approaches like the nuclear magnetic resonance spectroscopy (NMR) based quantification of metabolites, lipoproteins, and inflammation markers has helped to identify typical alterations in blood serum COVID-19 patients. However, confounders such as sex, comorbidities, which strongly influence metabolome, were often not considered. Therefore, aim this NMR study was consider well arterial hypertension (AHT), when investigating COVID-19-positive samples a large...
The complex manifestations of COVID-19 are still not fully decoded on the molecular level. We combined quantitative nuclear magnetic resonance (NMR) spectroscopy serum analysis metabolites, lipoproteins and inflammation markers with clinical parameters a targeted cytokine panel to characterize in large (534 patient samples, 305 controls) outpatient cohort recently tested PCR-positive patients. consisted patients who were predominantly initial phase disease mostly exhibited milder course....
We report comprehensive X-ray diffraction and NMR studies of potassium-induced dimerization heteroleptic triple-decker crown-phthalocyaninates [(15C5)4Pc]M(Pc)M(Pc) (1M, M = Y Tb). Characterization the crystalline dimer 2(1Y)·4KBPh4·12CH3CN·10CHCl3 gave first structural evidence formation a six-decker structure with four rare earth metal ions perfectly aligned near symmetry axis. soluble supramolecular dimers 2(1M)·4KOAc provided spectral–structural model that allowed us to assign spectra...
The SARS-CoV-2 virus is the causative agent of global COVID-19 infectious disease outbreak, which can lead to acute respiratory distress syndrome (ARDS). However, it still unclear how interferes with immune cell and metabolic functions in human body. In this study, we investigated response or convalescent patients. We characterized peripheral blood mononuclear cells (PBMCs) using flow cytometry found that CD8+ T were significantly subsided moderate Furthermore, characterization suggested...
Ambroxol is a well-known mucolytic expectorant, which has gained much attention in amyotrophic lateral sclerosis, Parkinson's and Gaucher's disease. A specific focus been placed on ambroxol's glucocerebrosidase-stimulating activity, grounds that the point mutation of gba1 gene, codes for this enzyme, risk factor developing However, ambroxol attributed other characteristics, such as potent inhibition sodium channels, modification calcium homeostasis, anti-inflammatory effects modifications...
Abstract Diet-induced increase in body weight is a growing health concern worldwide. Often accompanied by low-grade metabolic inflammation that changes systemic functions, diet-induced alterations may contribute to neurodegenerative disorder progression as well. This study aims non-invasively investigate and inflammatory effects the brain of an APPPS1 mouse model Alzheimer’s disease. [ 18 F]FDG, F]FTHA, F]GE-180 were used for vivo PET imaging wild-type mice. Ex flow cytometry histology...
Herein we report single-crystal X-ray diffraction characterization and complementary solution studies of supramolecular interaction between potassium salts heteroleptic homo- heteronuclear triple-decker crown phthalocyaninates [(15C5)4Pc]M*[(15C5)4Pc]M(Pc) or [M*,M], where M* M = Y and/or Tb. Our results evidence that, in contrast to the previously studied crown-substituted phthalocyanines, K+ cations with [M*,M] does not induce their intermolecular aggregation. Instead, reversibly...
Background: Traditional diagnosis is based on histology or clinical-stage classification which provides no information tumor metabolism and inflammation, which, however, are both hallmarks of cancer directly associated with prognosis severity. This project was an exploratory approach to profile metabolites, lipoproteins, inflammation parameters (glycoprotein A glycoprotein B) borderline ovarian (BOT) high-grade serous (HGSOC) for identifying additional useful serum markers stratifying...
We present compelling evidence for the existence of an extended innate viperin-dependent pathway, which provides crucial adaptive response to viral agents, such as SARS-CoV-2. show in vivo biosynthesis a family novel endogenous cytosine metabolites with potential antiviral activities. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed characteristic spin-system motif, indicating presence panel urinary during acute replication phase. Mass spectrometry additionally enabled...
Pancreatic ductal adenocarcinoma (PDAC) is difficult to diagnose in the early stages and lacks reliable biomarkers. The scope of this project was establish quantitative nuclear magnetic resonance (NMR) spectroscopy comprehensively study blood serum alterations PDAC patients. Serum samples from 34 patients obtained before after pancreatectomy as well 83 age- sex-matched control healthy donors were analyzed with vitro diagnostics research (IVDr) proton NMR at 600 MHz. Uni- multivariate...
The poor prognosis of ovarian cancer patients is strongly related to peritoneal metastasis with the production malignant ascites. However, it remains largely unclear how ascites in cavity influences tumor metabolism and recurrence. This study an explorative approach aimed at for a deeper molecular physical-chemical characterization investigate their effect on vitro cell proliferation.This included 10 specimens from undergoing resection. Ascites samples were deeply phenotyped by 1H-NMR based...
The stratification of individuals suffering from acute and post-acute SARS-CoV-2 infection remains a critical challenge. Notably, biomarkers able to specifically monitor viral progression, providing details about patient clinical status, are still not available. Herein, quantitative metabolomics is progressively recognized as useful tool describe the consequences virus-host interactions considering also metadata.
Metabolic syndrome (MetS) is a cluster of medical conditions and risk factors correlating with insulin resistance that increase the developing cardiometabolic health problems. The specific criteria for diagnosing MetS vary among different organizations but are typically based on evaluation abdominal obesity, high blood pressure, hyperglycemia, dyslipidemia. A unique, quantitative independent estimation only biomarkers highly desirable comparison between patients to study individual...
Abstract The SARS-CoV-2 virus is the causative agent of global COVID-19 infectious disease outbreak, which can lead to acute respiratory distress syndrome (ARDS). However, it still unclear how interferes with immune cell and metabolic functions in human body. In this study, we investigated response or convalescent COVID19 patients. We characterized peripheral blood mononuclear cells (PBMCs) using flow cytometry found that CD8 + T were significantly subsided moderate Furthermore,...
Abstract We present compelling evidence for the existence of an extended innate viperin dependent pathway which provides crucial adaptive response to viral agents like SARS-CoV-2. show in vivo biosynthesis a family endogenous cytosine metabolites with potential antiviral activity. Two dimensional Nuclear magnetic resonance (NMR) spectroscopy revealed characteristic spin-system motif indicating presence panel urinary during acute replication phase. Mass spectrometry additionally allowed...
Abstract Background The exact pathophysiology of humans suffering from the multifaceted SARS-CoV-2 infection is not yet conclusively understood and risk stratification needed. Novel diagnostic approaches like nuclear magnetic resonance spectroscopy (NMR) based quantification metabolites, lipoproteins, inflammation markers has helped to identify typical alterations in blood serum COVID-19 patients. However, important confounders such as age, sex, comorbidities, which strongly influence...
Abstract Deep metabolomic, proteomic and immunologic phenotyping of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) patients have matched a wide diversity clinical symptoms with potential biomarkers for disease 2019 (COVID-19). Within here, several studies described the role metabolites, lipoproteins inflammation markers during infection in recovered patients. In fact, after SARS-CoV-2 viral almost 20-30% experience persistent even 12 weeks recovery which has been defined as...
Abstract Diet-induced body weight gain is a growing health problem worldwide, leading to several serious systemic diseases such as diabetes. Because it often accompanied by low-grade metabolic inflammation that alters function, dietary changes may also contribute the progression of neurodegenerative diseases. Here we demonstrate disrupted glucose and fatty acid metabolism plasma metabolome in mouse model Alzheimer’s disease following western diet using multimodal imaging approach NMR-based...