A5033572764- Metabolomics and Mass Spectrometry Studies
- Long-Term Effects of COVID-19
- Liver Disease Diagnosis and Treatment
- Radical Photochemical Reactions
- COVID-19 Clinical Research Studies
- Infectious Encephalopathies and Encephalitis
- Pancreatic and Hepatic Oncology Research
- Burn Injury Management and Outcomes
- Intensive Care Unit Cognitive Disorders
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Photoreceptor and optogenetics research
- Thermal Regulation in Medicine
- Lipid Membrane Structure and Behavior
- Neuroendocrine Tumor Research Advances
- Spectroscopy Techniques in Biomedical and Chemical Research
- Pancreatitis Pathology and Treatment
- Diabetic Foot Ulcer Assessment and Management
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurological and metabolic disorders
- interferon and immune responses
- Esophageal and GI Pathology
- NMR spectroscopy and applications
- Wound Healing and Treatments
- Photochromic and Fluorescence Chemistry
Murdoch University
2020-2025
Harry Perkins Institute of Medical Research
2023-2024
Avenir Health
2020-2023
University of Regensburg
2018-2021
University Hospital Carl Gustav Carus
2016-2019
TU Dresden
2016-2019
Klinik und Poliklinik für Psychotherapie und Psychosomatik
2016-2018
Hôpital Necker-Enfants Malades
2015
The metabolic effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on human blood plasma were characterized using multiplatform phenotyping with nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography–mass spectrometry (LC-MS). Quantitative measurements lipoprotein subfractions, α-1-acid glycoprotein, glucose, biogenic amines made samples from symptomatic disease 19 (COVID-19) patients who had tested positive for the SARS-CoV-2 virus (n = 17) age-...
We present a multivariate metabotyping approach to assess the functional recovery of nonhospitalized COVID-19 patients and possible biochemical sequelae "Post-Acute Syndrome", colloquially known as long-COVID. Blood samples were taken from ca. 3 months after acute infection with further assessment symptoms at 6 months. Some 57% had one or more persistent including respiratory-related like cough, dyspnea, rhinorrhea other nonrespiratory chronic fatigue, anosmia, myalgia, joint pain. Plasma...
To investigate the systemic metabolic effects of SARS-CoV-2 infection, we analyzed 1H NMR spectroscopic data on human blood plasma and co-modeled with multiple cytokines chemokines (measured in parallel). Thus, 600 MHz solvent-suppressed single-pulse, spin-echo, 2D J-resolved spectra were collected recorded from rRT-PCR-positive patients (n = 15, sampling timepoints) age-matched healthy controls 34, confirmed rRT-PCR negative), together COVID-19/influenza-like clinical symptoms who tested...
We have applied nuclear magnetic resonance spectroscopy based plasma phenotyping to reveal diagnostic molecular signatures of SARS-CoV-2 infection via combined diffusional and relaxation editing (DIRE). compared from healthy age-matched controls (n = 26) with negative non-hospitalized respiratory patients hospitalized 23 11 respectively) rRT-PCR positive 17, longitudinal sampling time-points). DIRE data were modelled using principal component analysis orthogonal projections latent structures...
Quantitative plasma lipoprotein and metabolite profiles were measured on an autonomous community of the Basque Country (Spain) cohort consisting hospitalized COVID-19 patients (n = 72) a matched control group 75) Western Australian (WA) 17) SARS-CoV-2 positives 20) healthy controls using 600 MHz 1H nuclear magnetic resonance (NMR) spectroscopy. Spanish samples in two laboratories one-dimensional (1D) solvent-suppressed T2-filtered methods with vitro diagnostic quantification lipoproteins...
Abstract Synthetic applications in photochemistry are booming. Despite great progress the development of new reactions, mechanistic investigations still challenging. Therefore, we present a fully automated situ combination NMR spectroscopy, UV/Vis and illumination to allow simultaneous time‐resolved detection paramagnetic diamagnetic species. This optical fiber‐based setup enables first acquisition combined spectra photocatalysis, as demonstrated on conPET process. Furthermore, broad...
Quantitative nuclear magnetic resonance (NMR) spectroscopy of blood plasma is widely used to investigate perturbed metabolic processes in human diseases. The reliability biochemical data derived from these measurements dependent on the quality sample collection and exact preparation analysis protocols. Here, we describe systematically, impact variations information recovery quantitative proton (1H) NMR serum. effects variation tube sizes preservatives, successive freeze-thaw cycles, storage...
Understanding the distribution and variation in inflammatory markers is crucial for advancing our knowledge of processes evaluating their clinical utility diagnosing monitoring acute chronic disease. 1 H NMR spectroscopy blood plasma serum was applied to measure a composite panel based on phase glycoprotein signals (GlycA GlycB) sub-regions lipoprotein derived Supramolecular Phospholipid Composite (SPC , SPC 2 3 ) establish normal ranges two healthy, predominantly white cohorts from...
Abstract COVID-19 vaccines are crucial in reducing SARS-CoV-2 transmission and severe health outcomes. Despite widespread administration, their long-term systemic effects on human metabolism remain inadequately understood. This longitudinal study aims to evaluate IgG responses, 34 cytokines, 112 lipoproteins, 21 low-molecular-weight metabolites 33 individuals receiving two four vaccine doses. Changes metabolic profiles for the first 16 days post each dose of vaccine, up 480 post-initial...
We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates potential reimagine population level cardiovascular disease risk individual screening on blood sampling. Using a quantitative calibration approach, we obtained stable reproducible results from multiple sites, despite reduced spectral dispersion sensitivity at lower field strengths. Our study shows that 25...
The aim of this study was to test the hypothesis that intraoperative frozen section (FS) and re-resection results achieve R0 status are associated with different long-term outcomes in pancreatic cancer patients.Recent data have challenged survival benefit additional resection patients case positive FS clear pathological (PS).Patients who underwent surgery for exocrine malignancy curative intent were identified from a prospective database. Data stratified by margin (group I: FS-R0 → PS-R0;...
The utility of low sample volume in vitro diagnostic (IVDr) proton nuclear magnetic resonance (1H NMR) spectroscopic experiments on blood plasma for information recovery from limited availability or high value samples was exemplified using patients with SARS-CoV-2 infection and normal controls. 1H NMR spectra were obtained solvent-suppressed 1D, spin-echo (CPMG), 2-dimensional J-resolved (JRES) spectroscopy both 3 mm outer diameter SampleJet tubes (100 μL plasma) 5 (300 under conditions. We...
Proton nuclear magnetic resonance (NMR) N-acetyl signals (Glyc) from glycoproteins and supramolecular phospholipids composite peak (SPC) phospholipid quaternary nitrogen methyls in subcompartments of lipoprotein particles) can give important systemic metabolic information, but their absolute quantification is compromised by overlap with interfering resonances lipids themselves. We present a J-Edited DIffusional (JEDI) proton NMR spectroscopic approach to selectively augment the inflammatory...
An integrative multi-modal metabolic phenotyping model was developed to assess the systemic plasma sequelae of SARS-CoV-2 (rRT-PCR positive) induced COVID-19 disease in patients with different respiratory severity levels. Plasma samples from 306 unvaccinated were collected 2020 and classified into four levels ranging mild symptoms severe ventilated cases. These investigated using a combination quantitative Nuclear Magnetic Resonance (NMR) spectroscopy Mass Spectrometry (MS) platforms give...
We investigated plasma and serum blood derivatives from capillary microsamples (500 μL, MiniCollect tubes) corresponding venous (10 mL vacutainers). Samples 20 healthy participants were analyzed by 1H NMR, 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters SPC1, SPC2, SPC3 subfractions; 2 N-acetyl signals α-1-acid glycoprotein (Glyc), GlycA, GlycB; calculated parameters, SPC (total), SPC3/SPC2, Glyc (total) assessed. Using linear regression...
The desire to photocontrol molecular properties ranging from materials pharmacology using light as an external trigger with high spatiotemporal resolution led the development of a broad range photochromic scaffolds. Among them, azobenzenes are synthetically well accessible and show excellent fatigue resistance. Their vary substitution pattern for different heteroarenes. However, photochromism 3(5)-substituted-1H-pryazoles has not yet been investigated, although this compound class offers...
SARS-CoV-2 infection causes a significant reduction in lipoprotein-bound serum phospholipids give rise to supramolecular phospholipid composite (SPC) signals observed diffusion and relaxation edited 1H NMR spectra. To characterize the chemical structural components compartmental location of SPC understand further its possible diagnostic properties, we applied Statistical HeterospectroscopY n-dimensions (SHY-n) approach. This involved statistically linking series orthogonal measurements made...
A JEDI NMR pulse experiment incorporating relaxational, diffusional and J-modulation peak editing has been implemented for a low field (80 MHz proton resonance frequency) spectrometer system to measure quantitatively two recently discovered plasma markers of SARS-CoV-2 infection general inflammation. spectra capture unique signature biomarker signals from acetylated glycoproteins (Glyc) the supramolecular phospholipid composite (SPC) that are relatively enhanced by combination relaxation,...
The first example for the photocatalytic generation of a highly electrophilic intermediate that is not based on radical reactivity reported. single-electron reduction bench-stable and commercially available 4-(trifluoromethoxy)benzonitrile by an organic photosensitizer leads to its fragmentation into fluorophosgene benzonitrile. in situ generated was used preparation carbonates, carbamates, urea derivatives moderate excellent yields via intramolecular cyclization reaction. Transient...
Abstract Synthetic applications in photochemistry are booming. Despite great progress the development of new reactions, mechanistic investigations still challenging. Therefore, we present a fully automated situ combination NMR spectroscopy, UV/Vis and illumination to allow simultaneous time‐resolved detection paramagnetic diamagnetic species. This optical fiber‐based setup enables first acquisition combined spectra photocatalysis, as demonstrated on conPET process. Furthermore, broad...
Described antimicrobial resistance mechanisms enable bacteria to avoid the direct effects of antibiotics and can be monitored by in vitro susceptibility testing genetic methods. Here we describe a mechanism sulfamethoxazole that requires host metabolite for activity. Using combination evolution metabolic rescue experiments, identify an energy-coupling factor (ECF) transporter S component gene (thfT) enables Group A Streptococcus acquire extracellular reduced folate compounds. ThfT likely...
Globally, burns are a significant cause of injury that can substantial acute trauma as well lead to increased incidence chronic comorbidity and disease. To date, research has primarily focused on the systemic response severe injury, with little in literature reported impact nonsevere injuries (<15% total burn surface area; TBSA). elucidate metabolic consequences longitudinal plasma was collected from adults (n = 35) who presented at hospital admission, 6 week follow up. A cross-sectional...