A5084002751- Metabolomics and Mass Spectrometry Studies
- Burn Injury Management and Outcomes
- Long-Term Effects of COVID-19
- Biosimilars and Bioanalytical Methods
- Intensive Care Unit Cognitive Disorders
- COVID-19 Clinical Research Studies
- Wound Healing and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Advanced Proteomics Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Neurological and metabolic disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Thermal Regulation in Medicine
- Computational Drug Discovery Methods
- SARS-CoV-2 detection and testing
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Heme Oxygenase-1 and Carbon Monoxide
- Mass Spectrometry Techniques and Applications
- Adipose Tissue and Metabolism
- Forensic Entomology and Diptera Studies
- Bipolar Disorder and Treatment
- Tryptophan and brain disorders
- Influenza Virus Research Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
Murdoch University
2021-2025
Harry Perkins Institute of Medical Research
2021-2025
Avenir Health
2022-2024
We present a multivariate metabotyping approach to assess the functional recovery of nonhospitalized COVID-19 patients and possible biochemical sequelae "Post-Acute Syndrome", colloquially known as long-COVID. Blood samples were taken from ca. 3 months after acute infection with further assessment symptoms at 6 months. Some 57% had one or more persistent including respiratory-related like cough, dyspnea, rhinorrhea other nonrespiratory chronic fatigue, anosmia, myalgia, joint pain. Plasma...
We performed quantitative metabolic phenotyping of blood plasma in parallel with cytokine/chemokine analysis from participants who were either SARS-CoV-2 (+) (n = 10) or (-) 49). positivity was associated a unique phenotype and demonstrated complex systemic response to infection, including severe perturbations amino acid kynurenine pathways. Nine metabolites elevated strongly infection (quinolinic acid, glutamic nicotinic aspartic neopterin, kynurenine, phenylalanine, 3-hydroxykynurenine,...
Dysregulated lipid metabolism underpins many chronic diseases including cardiometabolic diseases. Mass spectrometry-based lipidomics is an important tool for understanding mechanisms of dysfunction and widely applied in epidemiology clinical studies. With ever-increasing sample numbers, single batch acquisition often unfeasible, requiring advanced methods that are accurate robust to batch-to-batch interday analytical variation. Herein, optimized comprehensive targeted workflow plasma serum...
Delayed diagnosis of patients with sepsis or septic shock is associated increased mortality and morbidity. UPLC-MS NMR spectroscopy were used to measure panels lipoproteins, lipids, biogenic amines, amino acids, tryptophan pathway metabolites in blood plasma samples collected from 152 within 48 h admission into the Intensive Care Unit (ICU) where 62 had no sepsis, 71 19 shock. Patients higher concentrations neopterin lower levels HDL cholesterol phospholipid particles comparison nonsepsis...
Improved methods are required for investigating the systemic metabolic effects of SARS-CoV-2 infection and patient stratification precision treatment. We aimed to develop an effective method using lipid profiles discriminating between infection, healthy controls, non-SARS-CoV-2 respiratory infections. Targeted liquid chromatography–mass spectrometry profiling was performed on discovery (20 SARS-CoV-2-positive; 37 controls; 22 COVID-19 symptoms but SARS-CoV-2negative) validation (312 100...
Dried blood spot (DBS) sample collections can offer a minimally invasive, cost-effective alternative to traditional venepuncture for remote sampling and high-frequency metabolic profiling. We present an optimized protocol DBS-based extraction comprehensive untargeted 4D lipid profiling using ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry (trapped ion mobility - spectrometry), designed support large-scale applications in population-wide lipidomics...
Abstract COVID-19 vaccines are crucial in reducing SARS-CoV-2 transmission and severe health outcomes. Despite widespread administration, their long-term systemic effects on human metabolism remain inadequately understood. This longitudinal study aims to evaluate IgG responses, 34 cytokines, 112 lipoproteins, 21 low-molecular-weight metabolites 33 individuals receiving two four vaccine doses. Changes metabolic profiles for the first 16 days post each dose of vaccine, up 480 post-initial...
Globally, burns are a significant cause of injury that can substantial acute trauma as well lead to increased incidence chronic comorbidity and disease. To date, research has primarily focused on the systemic response severe injury, with little in literature reported impact nonsevere injuries (<15% total burn surface area; TBSA). elucidate metabolic consequences longitudinal plasma was collected from adults (n = 35) who presented at hospital admission, 6 week follow up. A cross-sectional...
Abstract Globally, burns are a significant cause of injury that can substantial acute trauma as well lead to increased incidence chronic co-morbidity and disease. To date, research has primarily focused on the systemic response severe injury, with little in literature reported impact non-severe injuries (<15% total burn surface area; TBSA). elucidate metabolic consequences longitudinal plasma was collected from adults (n=35) who presented at hospital admission, 6 week follow up. A...
Abstract Background Non-severe paediatric burns can result in poor long-term health outcomes. This occurs even cases with good acute burn-related outcomes, including minimal scarring. The mechanisms that underpin the transition from non-severe burn to sustained negative impacts are currently unknown. However, metabolic and immune changes have been observed studies, suggesting these may be important. plasma lipidome consists of a rich pool bioactive metabolites play critical roles systemic...
Abstract Whilst wound repair in severe burns has received substantial research attention, non-severe (<20% total body surface area) remain relatively understudied, despite causing considerable physiological impact and constituting most of the hospital admissions for burns. Early prediction healing outcomes would decrease financial patient burden, aid preventing long-term complications from poor healing. Lipids have been implicated inflammation tissue may play essential roles burn In this...