A5008892569- Infant Nutrition and Health
- Breastfeeding Practices and Influences
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- Biosimilars and Bioanalytical Methods
- Long-Term Effects of COVID-19
- Intensive Care Unit Cognitive Disorders
- Pesticide Exposure and Toxicity
- Chemical Analysis and Environmental Impact
- Advanced Proteomics Techniques and Applications
- Toxic Organic Pollutants Impact
- Mass Spectrometry Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Neurological and metabolic disorders
- Erythropoietin and Anemia Treatment
- Child Nutrition and Water Access
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Pediatric health and respiratory diseases
- Clinical Nutrition and Gastroenterology
- Cynara cardunculus studies
- Tryptophan and brain disorders
- Analytical Chemistry and Chromatography
- Plant Growth Enhancement Techniques
- Per- and polyfluoroalkyl substances research
The University of Western Australia
2016-2023
Institut de Biologie Moléculaire et Cellulaire
2018
Institut thématique Génétique, génomique et bioinformatique
2018
ENT and Allergy
2018
University of Lausanne
2018
Singapore Science Park
2015
Murdoch University
2009
Établissement Français du Sang
1979
We present a multivariate metabotyping approach to assess the functional recovery of nonhospitalized COVID-19 patients and possible biochemical sequelae "Post-Acute Syndrome", colloquially known as long-COVID. Blood samples were taken from ca. 3 months after acute infection with further assessment symptoms at 6 months. Some 57% had one or more persistent including respiratory-related like cough, dyspnea, rhinorrhea other nonrespiratory chronic fatigue, anosmia, myalgia, joint pain. Plasma...
We performed quantitative metabolic phenotyping of blood plasma in parallel with cytokine/chemokine analysis from participants who were either SARS-CoV-2 (+) (n = 10) or (-) 49). positivity was associated a unique phenotype and demonstrated complex systemic response to infection, including severe perturbations amino acid kynurenine pathways. Nine metabolites elevated strongly infection (quinolinic acid, glutamic nicotinic aspartic neopterin, kynurenine, phenylalanine, 3-hydroxykynurenine,...
Human milk provides essential substrates for the optimal growth and development of a breastfed infant. Besides providing nutrients to infant, human also contains metabolites which form an intricate system between maternal lifestyle, such as mother's diet gut microbiome, infant outcomes. This study investigates variation these from five different countries. samples (n = 109) were collected one month postpartum Australia, Japan, USA, Norway, South Africa analyzed by nuclear magnetic resonance....
Short chain fatty acids (SFCAs) are microbial metabolites produced in the gut upon fermentation of dietary fibre. These interact with host immune system and can elicit epigenetic effects. There is evidence to suggest that SCFAs may play a role developmental programming disorders obesity, though humans remains sparse. Here we have quantified human milk (HM) SCFA levels an international cohort atopic non-atopic mothers (n = 109). Our results demonstrate contains detectable acetate, butyrate,...
Abstract Human milk provides the infant with essential nutritive and non-nutritive factors required for health, growth development. The human lipidome is complex, but comprises predominantly triacylglycerides. Historically, fatty acid profile of entire has been investigated, many relationships have identified between health acids. Most these acids are, however, delivered to as Using liquid chromatography-ion mobility-mass spectrometry, objective this study was characterise triacylglyceride...
We investigated plasma and serum blood derivatives from capillary microsamples (500 μL, MiniCollect tubes) corresponding venous (10 mL vacutainers). Samples 20 healthy participants were analyzed by 1H NMR, 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters SPC1, SPC2, SPC3 subfractions; 2 N-acetyl signals α-1-acid glycoprotein (Glyc), GlycA, GlycB; calculated parameters, SPC (total), SPC3/SPC2, Glyc (total) assessed. Using linear regression...
Dried blood spot (DBS) sample collections can offer a minimally invasive, cost-effective alternative to traditional venepuncture for remote sampling and high-frequency metabolic profiling. We present an optimized protocol DBS-based extraction comprehensive untargeted 4D lipid profiling using ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry (trapped ion mobility - spectrometry), designed support large-scale applications in population-wide lipidomics...
Metabolic phenotyping using mass spectrometry (MS) is being applied to ever increasing sample numbers in clinical and epidemiology studies. High-throughput robust methods are developed for the accurate measurement of metabolites associated with disease. Traditionally, quantitative assays have utilized triple quadrupole (QQQ) MS based methods; however, use such focused removes ability perform discovery-based metabolic phenotyping. An integrated workflow hybrid simultaneous quantification 34...
Abstract The presence of pesticides in human milk (HM) is great concern due to the potential health effects for breastfed infant. To determine relationships between HM and infant growth development, a longitudinal study was conducted. samples (n = 99) from 16 mothers were collected at 2, 5, 9 12 months lactation. A validated QuEChERS method Gas chromatography-tandem mass spectrometry (GC-MS/MS) used analysis 88 HM. Only p,p’ -DDE, -DDT β -HCH detected with mean concentration (±SD) 52.25 ±...
Human milk (HM) lipid content is highly variable, and infants consume different volumes of milk. This makes precise sampling calculation the infant intake problematic. In order to describe inaccuracies estimates introduced by various protocols, we compared true with estimated intakes using protocols. Monthly samples (n = 1026) from months 1 6 lactation were collected 20 healthy, exclusively breastfeeding women. Infant was measured 24-hour test-weighing at month 3. Total creamatocrit....
The fatty acids (FAs) of human milk (HM) are the building blocks HM lipidome, contributing to infant health and development; however, this has not been comprehensively characterised with respect intake. Eighteen Western Australian mother-infant dyads provided monthly longitudinal samples during six months exclusive breastfeeding. Monthly anthropometric measurements, data basic maternal food frequency were also collected. At three months, 24 h intake total lipid measured. FA profile was...
Microsamples are collections usually less than 50 µL, although all devices that we have captured as part of this review do not fit within definition (as some can perform up to 600 µL); however, they considered microsamples be self-administered. These been introduced in pre-clinical, clinical, and research settings overcome obstacles sampling via traditional venepuncture. However, venepuncture remains the gold standard for metabolic phenotyping blood. This presents several challenges...
Inulin, a polysaccharide characterized by β-2,1 fructosyl-fructose structure terminating in glucosyl moiety, is naturally present plant roots and tubers. Current methods provide average degrees of polymerization (DP) but lack information on the distribution absolute concentration each DP. To address this limitation, reproducible (CV < 10 %) high throughput (<2 min/sample) MALDI-MRMS approach capable characterizing quantifying inulin molecules plants using matched-matrix consisting...
Hepcidin has been identified as the principle regulatory hormone essential for iron homeostasis. Quantitative analysis of hepcidin in bodily fluids provides an insight into pathogenesis disorders metabolism such hereditary hemochromatosis and anemia chronic disease. This study describes use solid phase extraction (SPE) a preparative step followed by matrix assisted laser desorption/ionization-orthogonal-time-of-flight mass spectrometry (MALDI-TOF MS) with internal standard quantitative...
The human milk fat globule membrane (MFGM) contains important lipids for growing infants. Anthropometric measurements, samples, and infant intake were collected in a cohort of eleven healthy mother–infant dyads during exclusive breastfeeding from birth to six months. One hundred sixty-six MFGM analysed using liquid chromatography-mass spectrometry, the was calculated. concentrations compared associations between growth characteristics explored. lipid varied widely months one three. many...
Small for gestational age (SGA) infants are those born small their age, with weight below the 10th percentile. Not only do SGA suffer growth issues after birth, they have elevated risk development of metabolic and cardiovascular diseases later in life. Current research has suggested that cases infants, maternal milk breastfeeding not affected. The mother an infant was diagnosed placental insufficiency Gestational Diabetes Mellitus (GDM) during her pregnancy. term, at 38 weeks 3 days, SGA....
Microsamples (collections usually less than 50 &micro;L) have been introduced in pre-clinical, clinical, and research settings to overcome obstacles sampling via traditional venipuncture. However, venipuncture remains the gold standard for metabolic phenotyping of blood. This pre-sents several challenges workflows: accessibility individuals ru-ral remote underserved areas (due need trained personnel), unamenable nature frequent protocols longitudinal (for its invasive nature), sample...