A5028997907- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Orthopedic Infections and Treatments
- Medical Imaging Techniques and Applications
- COVID-19 Clinical Research Studies
- Infective Endocarditis Diagnosis and Management
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 and COVID-19 Research
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Peptidase Inhibition and Analysis
- Bacterial Infections and Vaccines
- Long-Term Effects of COVID-19
- Clostridium difficile and Clostridium perfringens research
- Immune cells in cancer
- Radiopharmaceutical Chemistry and Applications
- Biosensors and Analytical Detection
- Sarcoidosis and Beryllium Toxicity Research
- Immune responses and vaccinations
- RNA regulation and disease
- Pneumonia and Respiratory Infections
- interferon and immune responses
- Amoebic Infections and Treatments
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2016-2025
Hospital of the University of Pennsylvania
2025
University of Chicago
2025
Mayo Clinic
2025
California University of Pennsylvania
2024
Imperial College London
2024
Pediatrics and Genetics
2017-2022
Biology of Infection
2017
Hospital Universitario de Santander
2016
2-[ 18 F]-Fluorodeoxysorbitol, a synthetic PET probe, can be used to rapidly, sensitively, and specifically image Gram-negative bacterial infections in animals.
It is unclear why some SARS-CoV-2 patients readily resolve infection while others develop severe disease. By interrogating metabolic programs of immune cells in and recovered coronavirus disease 2019 (COVID-19) compared with other viral infections, we identify a unique population T cells. These express increased Voltage-Dependent Anion Channel 1 (VDAC1), accompanied by gene functional characteristics linked to mitochondrial dysfunction apoptosis. The percentage these increases elderly...
Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), has incited a global health crisis. Currently, there are limited therapeutic options for prevention and treatment SARS-CoV-2 infections. We evaluated antiviral activity sulforaphane (SFN), principal biologically active phytochemical derived from glucoraphanin, naturally occurring precursor present in high concentrations cruciferous vegetables. SFN inhibited vitro...
The modern patient is increasingly susceptible to bacterial infections including those due multidrug-resistant organisms (MDROs). Noninvasive whole-body analysis with pathogen-specific imaging technologies can significantly improve outcomes by rapidly identifying a source of infection and monitoring the response treatment, but no such technology exists clinically.We systematically screened 961 random radiolabeled molecules in silico as substrates for essential metabolic pathways bacteria,...
Bacterial biofilm formation is a major complication of implantable medical devices that results in therapeutically challenging chronic infections, especially cases involving antibiotic-resistant bacteria. As an approach to prevent these electrospun composite coating comprised poly(lactic-coglycolic acid) (PLGA) nanofibers embedded poly(ε-caprolactone) (PCL) film was developed locally codeliver combinatorial antibiotics from the implant surface. The release each antibiotic could be adjusted...
Men experience more severe outcomes from coronavirus disease 2019 (COVID-19) than women. Golden Syrian hamsters were used to explore sex differences in the pathogenesis of a human clinical isolate acute respiratory syndrome 2 (SARS-CoV-2).
BACKGROUND. Recent studies have reported T cell immunity to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in unexposed donors, possibly due crossrecognition by cells specific for common cold coronaviruses (CCCs). True crossreactivity, defined as recognition a single TCR of more than one distinct peptide-MHC ligand, has never been shown context SARS-CoV-2.
18 F-FDS PET/CT can detect and localize infections due to Enterobacterales in patients noninvasively monitor response antibiotic treatment.
Background . India has one of the highest tuberculosis (TB) burdens globally. However, few studies have focused on TB in young children, a vulnerable population, where lack early diagnosis results poor outcomes. Methods Young children (≤5 years) with suspected were prospectively enrolled at tertiary hospital Pune, India. Detailed clinical evaluation, HIV testing, mycobacterial cultures, and drug susceptibility testing performed. Results 223 enrolled. The median age was 31 months, 46% female,...
PET reveals spatially heterogeneous rifampin brain penetration, which decreases rapidly with treatment during tuberculous meningitis.
Staphylococcus aureus is the leading cause of life-threatening infections, frequently originating from unknown or deep-seated foci. Source control and institution appropriate antibiotics remain challenges, especially with infections due to methicillin-resistant S. (MRSA). In this study, we developed a radiofluorinated analog para-aminobenzoic acid (2-[18F]F-PABA) demonstrate that it an efficient alternative substrate for dihydropteroate synthase (DHPS). 2-[18F]F-PABA rapidly accumulated in...
Imaging studies are frequently used to support the clinical diagnosis of infection. These techniques include computed tomography (CT) and magnetic resonance imaging (MRI) for structural information single photon emission (SPECT) or positron (PET) metabolic data. However, frequently, there is significant overlap in appearance infectious noninfectious entities using these tools. To address this concern, recent approaches have targeted bacteria-specific pathways. For example, radiolabeled...
Cavitation is a key pathological feature of human tuberculosis (TB) and well-recognized risk factor for transmission infection, relapse, emergence drug resistance after treatment. Despite intense interest in the mechanisms underlying cavitation its negative impact on treatment outcomes, there has been limited study this phenomenon due large part to limitations existing animal models. Although does not occur conventional mouse strains infection with Mycobacterium tuberculosis, cavitary lung...
Tuberculosis (TB) is a top-ten cause of death worldwide. Successful treatment often limited by insufficient diagnostic capabilities, especially at the point care in low-resource settings. The ideal must be fast, cheap, and require minimal clinical resources while providing high sensitivity, selectivity, ability to differentiate live from dead bacteria. We describe here development luminescent, affordable sensor Hip1 (FLASH) for detecting monitoring drug susceptibility Mycobacterium...
Pretomanid is a nitroimidazole antimicrobial active against drug-resistant Mycobacterium tuberculosis and approved in combination with bedaquiline linezolid (BPaL) to treat multidrug-resistant (MDR) pulmonary (TB). However, the penetration of these antibiotics into central nervous system (CNS), efficacy BPaL regimen for TB meningitis, are not well established. Importantly, there lack efficacious treatments meningitis due MDR strains, resulting high mortality. We have developed new methods...
ABSTRACT Tuberculosis (TB) is a global pandemic requiring sustained therapy to facilitate curing and prevent the emergence of drug resistance. There are few adequate tools evaluate dynamics within infected tissues in vivo . In this report, we evaluated fluorinated analog isoniazid (INH), 2-[ 18 F]fluoroisonicotinic acid hydrazide (2-[ F]-INH), as probe for imaging Mycobacterium tuberculosis -infected mice by dynamic positron emission tomography (PET). We developed tail vein catheter system...
ABSTRACT Current tools for monitoring response to tuberculosis treatments have several limitations. Noninvasive biomarkers could accelerate drug development and clinical studies, but date little progress has been made in developing new imaging technologies this application. In study, we developed pulmonary single-photon emission computed tomography (SPECT) using radioiodinated DPA-713 serially monitor the activity of live mice, which develop necrotic granulomas cavitary lesions. C3HeB/FeJ...
Central nervous system (CNS) tuberculosis (TB) is the most severe form of extra-pulmonary TB and disproportionately affects young children where developing brain has a unique host response. New Zealand white rabbits were infected with Mycobacterium via subarachnoid inoculation at postnatal day 4-8 evaluated until 4-6 weeks post-infection. Control rabbit kits assessed for development neurological deficits, bacterial burden, postmortem microbiologic pathologic changes. The presence meningitis...
The 6th annual meeting to address key issues in positron emission tomography (PET)/magnetic resonance imaging (MRI) was held again Tübingen, Germany, from March 27 29, 2017. Over three days of invited plenary lectures, round table discussions and dialogue board deliberations, participants critically assessed the current state PET/MRI, both clinically as a research tool, attempted chart future directions. addressed use PET/MRI workflows oncology, neurosciences, infection, inflammation chronic...
A rabbit model of pulmonary cavitary tuberculosis helps to explain phase 2 clinical trial results for the rifamycin class TB drugs.