A5009487218- Cancer, Hypoxia, and Metabolism
- Advanced MRI Techniques and Applications
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- MRI in cancer diagnosis
- Medical Imaging Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Lanthanide and Transition Metal Complexes
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Inflammatory mediators and NSAID effects
- RNA Interference and Gene Delivery
- Angiogenesis and VEGF in Cancer
- Radiopharmaceutical Chemistry and Applications
- Radiomics and Machine Learning in Medical Imaging
- Advanced NMR Techniques and Applications
- Diet and metabolism studies
- Virus-based gene therapy research
- Chemokine receptors and signaling
- Electron Spin Resonance Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Amino Acid Enzymes and Metabolism
Johns Hopkins Medicine
2016-2025
Johns Hopkins University
2016-2025
Sidney Kimmel Comprehensive Cancer Center
2015-2024
UNICEF East Asia and Pacific Regional Office
2018-2024
Continental (Germany)
2012-2024
Institute for Biomedicine
2012-2024
Morgan State University
2012-2022
University of Baltimore
2012-2022
Imaging Center
2003-2018
's Heeren Loo
2018
The switch to an angiogenic phenotype is a fundamental determinant of neoplastic growth and tumor progression. We demonstrate that homozygous deletion the p53 suppressor gene via homologous recombination in human cancer cell line promotes neovascularization xenografts nude mice. find Mdm2-mediated ubiquitination proteasomal degradation HIF-1α subunit hypoxia-inducible factor 1 (HIF-1), heterodimeric transcription regulates cellular energy metabolism angiogenesis response oxygen deprivation....
Abstract Purpose: Modern imaging technologies such as CT, PET, SPECT, and MRI employ contrast agents to visualize the tumor microenvironment, providing information on malignancy response treatment. Currently, all clinical require chemical labeling, i.e. with iodine (CT), radioisotopes (PET/SPECT), or paramagnetic metals (MRI). The goal was explore possibility of using simple D ‐glucose an infusable biodegradable agent for cancer detection. Methods: signals were detected exchange saturation...
MR molecular imaging is an exciting new frontier in the biomedical applications of MR. One clinically relevant targets tyrosine kinase Her-2/neu receptor, which has a significant role staging and treating breast cancer. In this study receptors were imaged panel cancer cells expressing different numbers on cell membrane. Commercially available streptavidin-conjugated superparamagnetic nanoparticles used as targeted contrast agent. The directed to prelabeled with biotinylated monoclonal...
Abstract Proton magnetic resonance spectroscopy (1H MRS) consistently detects significant differences in choline phospholipid metabolites of malignant versus benign breast lesions. It is critically important to understand the molecular causes underlying these metabolic differences, because this may identify novel targets for attack cancer cells. In study, membrane metabolism were characterized cells and normal human mammary epithelial (HMECs) labeled with [1,2-13C]choline, using 1H 13C...
The extracellular pH (pHex) of tumors is generally acidic. However, it only recently that noninvasive magnetic resonance spectroscopic (MRS) measurements have determined the intracellular (pHin) tumor cells in situ neutral or slightly alkaline compared with normal tissues. Thus maintain larger gradients than do nontumor To date, pHex been made using microelectrodes, which preclude measurement and pHin within same preparation. In addition, microelectrodes are invasive potential to alter...
Tumor pH is physiologically important since it influences a number of processes relevant to tumorigenesis and therapy. Hence, knowledge localized within tumors would contribute understanding these processes. The destructiveness, poor spatial resolution, signal-to-noise ratio (SNR) current technologies (e.g., microelectrodes, 31P magnetic resonance spectroscopy) have limited such studies. An extrinsic chemical extracellular (pHe) probe described that used in combination with 1H spectroscopic...
The extracellular (interstitial) pH (pHe) of solid tumours is significantly more acidic compared to normal tissues. In-vitro, low reduces the uptake weakly basic chemotherapeutic drugs and, hence, their cytotoxicity. This phenomenon has been postulated contribute a 'physiological' resistance in vivo. Doxorubicin weak base agent that commonly used combination chemotherapy clinically treat breast cancers. report demonstrates MCF-7 human cancer cells vitro are susceptible doxorubicin toxicity...
Abstract Aggressive cancer phenotypes are a manifestation of many different genetic alterations that promote rapid proliferation and metastasis. In this study, we show stable overexpression Twist in breast cell line, MCF-7, altered its morphology to fibroblastic-like phenotype, which exhibited protein markers representative mesenchymal transformation. addition, it was observed MCF-7/Twist cells had increased vascular endothelial growth factor (VEGF) synthesis when compared with empty vector...
Cancer cells invade by secreting degradative enzymes, which are sequestered in lysosomal vesicles. In this study, the impact of an acidic extracellular environment on lysosome size, number, and distance from nucleus human mammary epithelial (HMECs) breast cancer different degrees malignancy was characterized because physiological microenvironment tumors is frequently acidity. An pH (pH(e)) resulted a distinct shift lysosomes perinuclear region to cell periphery irrespective HMECs' degree...
Abstract Purpose To investigate the diagnostic value of proton magnetic resonance spectroscopic imaging (MRSI) in patients with breast lesions. Materials and Methods Eighteen underwent MRSI MRI at 1.5 T. Contrast‐enhanced MR was used to identify lesion, after which single‐slice (TR/TE = 2000/272 msec, 10‐mm slice thickness) performed. Water, lipid, choline (Cho) images were reconstructed from data. The area Cho measured lesion expressed relative background noise level (signal‐to‐noise ratio...
A high-performance magnetic resonance imaging T-2 contrast agent has been prepared via phase transfer of hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) to an aqueous solution by us ...
Altered phosphatidylcholine (PC) metabolism in epithelial ovarian cancer (EOC) could provide choline-based imaging approaches as powerful tools to improve diagnosis and identify new therapeutic targets. The increase the major choline-containing metabolite phosphocholine (PCho) EOC compared with normal nontumoral immortalized counterparts (EONT) may derive from (a) enhanced choline transport kinase (ChoK)-mediated phosphorylation, (b) increased PC-specific phospholipase C (PC-plc) activity,...
Otto Warburg's classic treatise on the reprogramming of tumour metabolism from oxidative to glycolytic was published in London 1930. Although Warburg effect is one most universal characteristics solid tumours, molecular basis for this phenomenon has only recently been elucidated by studies indicating that increased expression genes encoding glucose transporters and enzymes cells mediated transcription factors c-MYC HIF-1. Whereas c-myc a direct target oncogenic mutations, hypoxia-inducible...
Abstract Choline kinase is overexpressed in breast cancer cells and activated by oncogenes mitogenic signals, making it a potential target for therapy. Here, we have examined, the first time, effects of RNA interference (RNAi)–mediated down-regulation choline nonmalignant malignant human epithelial cell lines using magnetic resonance spectroscopy (MRS) as well molecular analyses proliferation differentiation markers. RNAi knockdown reduced proliferation, detected proliferating nuclear...
Glutamine addiction in c-MYC-overexpressing breast cancer is targeted by the aminotransferase inhibitor, aminooxyacetate (AOA). However, mechanism of ensuing cell death remains unresolved.A correlation between glutamine dependence for growth and c-MYC expression was studied lines. The cytotoxic effects AOA, its with high expression, on enzymes glutaminolytic pathway were investigated. AOA-induced assessed measuring changes metabolite levels magnetic resonance spectroscopy (MRS), amino acid...