A5027774891- Prostate Cancer Treatment and Research
- Radiopharmaceutical Chemistry and Applications
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Medical Imaging Techniques and Applications
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Infectious Diseases and Tuberculosis
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Tuberculosis Research and Epidemiology
- Orthopedic Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Bacterial Infections and Vaccines
- Neuroscience and Neuropharmacology Research
- Immune Cell Function and Interaction
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2006-2024
University of Baltimore
2005-2021
Memorial Sloan Kettering Cancer Center
2019
Korea University
2011
Kennedy Krieger Institute
2009
Galaxy Biotech (United States)
2009
University of Notre Dame
2009
University of Illinois Chicago
2008
Toshima Hospital
2007
We have synthesized and evaluated in vivo 2-(3-{1-carboxy-5-[(6-[(18)F]fluoro-pyridine-3-carbonyl)-amino]-pentyl}-ureido)-pentanedioic acid, [(18)F]DCFPyL, as a potential imaging agent for the prostate-specific membrane antigen (PSMA). PSMA is upregulated prostate cancer epithelia neovasculature of most solid tumors.[(18)F]DCFPyL was two steps from p-methoxybenzyl (PMB) protected lys-C(O)-glu urea precursor using 6-[(18)F]fluoronicotinic acid tetrafluorophenyl ester ([(18)F]F-Py-TFP)...
Prostate-specific membrane antigen (PSMA) is expressed in normal human prostate epithelium and highly up-regulated cancer. We previously reported a series of novel small molecule inhibitors targeting PSMA. Two compounds, MIP-1072, (S)-2-(3-((S)-1-carboxy-5-(4-iodobenzylamino)pentyl)ureido)pentanedioic acid, MIP-1095, (S)-2-(3-((S)-1carboxy-5-(3-(4-iodophenyl)ureido)pentyl)ureido)pentanedioic were selected for further evaluation. MIP-1072 MIP-1095 potently inhibited the glutamate...
Significance [ 11 C]CPPC [5-cyano- N -(4-(4-[ C]methylpiperazin-1-yl)-2-(piperidin-1-yl)phenyl)furan-2-carboxamide] is a PET radiotracer specific for CSF1R, microglia-specific marker. This compound can be used as noninvasive tool imaging of reactive microglia, disease-associated microglia and their contribution to neuroinflammation in vivo. Neuroinflammation posited an underlying pathogenic feature wide variety neuropsychiatric disorders. may also study specifically the immune environment...
Abstract Purpose: Prostate-specific membrane antigen (PSMA) is a cell surface protein that overexpressed in prostate cancer, including hormone-refractory and metastatic disease. Our goal this study was to develop series of PSMA-based imaging agents for clinical use. Experimental Design: We have synthesized evaluated the vivo biodistribution two radiolabeled urea derivatives high affinity PSMA severe combined immunodeficient mice harboring MCF-7 (breast, PSMA-negative), PC-3 (prostate, LNCaP...
The prostate-specific membrane antigen (PSMA) is increasingly recognized as a viable target for imaging and therapy of cancer. We prepared seven 99mTc/Re-labeled compounds by attaching known Tc/Re chelating agents to an amino-functionalized PSMA inhibitor (lys-NHCONH-glu) with or without variable length linker moiety. Ki values ranged from 0.17 199 nM. Ex vivo biodistribution in demonstrated the degree specific binding engineered PSMA+ PC3 PIP tumors. PC3-PIP cells are derived that have been...
Abstract Purpose: Previously, we showed successful imaging of xenografts that express the prostate-specific membrane antigen (PSMA) using small-animal positron emission tomography (PET) and radiolabeled PSMA inhibitor N-[N-[(S)-1,3-dicarboxypropyl]carbamoyl]-S-[11C]methyl-l-cysteine. Herein, extend work by preparing testing a same class labeled with fluorine-18. Experimental Design: N-[N-[(S)-1,3-Dicarboxypropyl]carbamoyl]-4-[18F]fluorobenzyl-l-cysteine ([18F]DCFBC) was prepared reacting...
To extend our development of new imaging agents targeting the prostate-specific membrane antigen (PSMA), we have used versatile intermediate 2-[3-(5-amino-1-carboxy-pentyl)-ureido]-pentanedioic acid (Lys-C(O)-Glu), which allows ready incorporation radiohalogens for single photon emission computed tomography (SPECT) and positron (PET). We prepared 2-[3-[1-carboxy-5-(4-[125I]iodo-benzoylamino)-pentyl]-ureido]-pentanedioic ([125I]3),...
Collagen remodeling is an integral part of tissue development, maintenance, and regeneration, but excessive associated with various pathologic conditions. The ability to target collagens undergoing could lead new diagnostics therapeutics as well applications in regenerative medicine; however, such are often degraded denatured, making them difficult conventional approaches. Here, we present caged collagen mimetic peptides (CMPs) that can be photo-triggered fold into triple helix bind...
Prostate-specific membrane antigen (PSMA) or folate hydrolase 1 (FOLH1) is highly expressed on prostate cancer. Its expression correlates inversely with survival and increases tumor grade. However, the biological role of PSMA has not been explored, its in cancer remained elusive. Filling this gap, we demonstrate that cancer, initiates signaling upstream PI3K through G protein–coupled receptors, specifically via metabotropic glutamate receptor (mGluR). PSMA’s carboxypeptidase activity...
Prostate-specific membrane antigen (PSMA) is a well-recognized target for identification and therapy of variety cancers. Here we report five 64Cu-labeled inhibitors PSMA, [64Cu]3–7, which are based on the lysine–glutamate urea scaffold utilize macrocyclic chelators, namely NOTA(3), PCTA(4), Oxo-DO3A(5), CB-TE2A(6), DOTA(7), in an effort to determine provides most suitable pharmacokinetics vivo PET imaging. [64Cu]3–7 were prepared high radiochemical yield (60–90%) purity (>95%). Positron...
Auger electron emitters such as <sup>125</sup>I have a high linear energy transfer and short range of emission (<10 μm), making them suitable for treating micrometastases while sparing normal tissues. We used highly specific small molecule targeting the prostate-specific membrane antigen (PSMA) to deliver prostate cancer cells. <b>Methods:</b> The PSMA-targeting emitter 2-[3-[1-carboxy-5-(4-<sup>125</sup>I-iodo-benzoylamino)-pentyl]-ureido]-pentanedioic acid (<sup>125</sup>I-DCIBzL) was...
Bacterial infections provide diagnostic dilemmas that could be enlightened by modern imaging technologies. We have developed a simple method for bacterial in mice relies on the phosphorylation and trapping of thymidine kinase (TK) substrate 1-(2′-deoxy-2′-fluoro-β- d -arabinofuranosyl)-5-[ 125 I] iodouracil ([ I]FIAU) within bacteria. FIAU was found to inhibit growth WT Escherichia coli but not TK – strain, indicating E. metabolize this compound. In silico analyses demonstrated all...
One reagent for two techniques: Compound [111In]1 provides a platform sequential radionuclide and optical imaging of prostate cancer through targeting the prostate-specific membrane antigen. Because same subject can be dual-imaged after one injection agent, these findings support rapid clinical translation. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by...
Abstract Background Transmigration of circulating dendritic cells (DCs) into the central nervous system (CNS) across blood–brain barrier (BBB) has not thus far been investigated. An increase in immune cell infiltration BBB, uncontrolled activation and antigen presentation are influenced by chemokines. Chemokine ligand 2 (CCL2) is a potent chemoattractant known to be secreted BBB but implicated recruitment DCs specifically at BBB. Methods Experimental autoimmune encephalomyelitis (EAE) was...
Cognitive dysfunction has been reported in patients with spinal cord injury (SCI), but it questioned whether such changes may reflect concurrent head injury, and the issue not addressed mechanistically or a well-controlled experimental model. Our recent rodent studies examining SCI-induced hyperesthesia revealed neuroinflammatory only supratentorial pain-regulatory sites, also other brain regions, suggesting that additional functions be impacted following SCI. Here we examined effects of...
Abstract Surgery is an efficient way to treat localized prostate cancer (PCa), however, it challenging demarcate rapidly and accurately the tumor boundary intraoperatively, as existing detection methods are seldom performed in real-time. To overcome those limitations, we develop a fluorescent molecular rotor that specifically targets prostate-specific membrane antigen (PSMA), established marker for PCa. The probes have picomolar affinity (IC 50 = 63-118 pM) PSMA generate virtually...
Clostridium novyi-NT (C. novyi-NT) spores have been shown to be potent therapeutic agents in experimental tumors of mice and rabbits. In the present study, pharmacologic toxicologic studies were performed better understand factors influencing efficacy toxicity this form therapy. We found that rapidly cleared from circulation by reticuloendothelial system. Even after large doses administered, no clinical was observed healthy or The also not toxic harboring poorly vascularized non-neoplastic...
Background Traditional imaging techniques for the localization and monitoring of bacterial infections, although reasonably sensitive, suffer from a lack specificity. This is particularly true musculoskeletal infections. Bacteria possess thymidine kinase (TK) whose substrate specificity distinct that major human TK. The difference has been exploited to develop new technique can detect presence viable bacteria. Methodology/Principal Findings Eight subjects with suspected infections one healthy...
Technetium-99m, the most commonly used radionuclide in nuclear medicine, can be attached to biologically important molecules through a variety of chelating agents, choice which depends upon imaging application. The prostate-specific membrane antigen (PSMA) is increasingly recognized as an target for and therapy prostate cancer (PCa). Three different (99m)Tc-labeling methods were employed investigate effect chelator on biodistribution PCa tumor uptake profiles 12 new urea-based PSMA-targeted...
ABSTRACT Current tools for monitoring response to tuberculosis treatments have several limitations. Noninvasive biomarkers could accelerate drug development and clinical studies, but date little progress has been made in developing new imaging technologies this application. In study, we developed pulmonary single-photon emission computed tomography (SPECT) using radioiodinated DPA-713 serially monitor the activity of live mice, which develop necrotic granulomas cavitary lesions. C3HeB/FeJ...