A5068593672- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Cytomegalovirus and herpesvirus research
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Polyomavirus and related diseases
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- CNS Lymphoma Diagnosis and Treatment
- Parvovirus B19 Infection Studies
- Renal Transplantation Outcomes and Treatments
- Acute Lymphoblastic Leukemia research
- Herpesvirus Infections and Treatments
- CAR-T cell therapy research
- T-cell and Retrovirus Studies
- Epigenetics and DNA Methylation
- Histiocytic Disorders and Treatments
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Eosinophilic Disorders and Syndromes
- Cancer-related gene regulation
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Pneumocystis jirovecii pneumonia detection and treatment
Johns Hopkins University
2016-2025
Sidney Kimmel Comprehensive Cancer Center
2016-2025
Johns Hopkins Medicine
2016-2025
Sidney Kimmel Cancer Center
2010-2023
University of Baltimore
1997-2023
U-M Rogel Cancer Center
2022
Comprehensive Blood & Cancer Center
2022
National Heart Lung and Blood Institute
2022
Johns Hopkins Hospital
1986-2021
Laboratory of Molecular Genetics
1999-2017
Primary central nervous system lymphoma occurs more often in patients with AIDS. Epstein-Barr virus (EBV) has been detected these tumours, but the degree of association not defined because both highly restricted expression EBV malignant tissue and lack a technique that is reliable formalin-fixed paraffin-embedded specimens. EBV-transformed lymphocytes contain short non-protein coding transcripts (EBERs), which are expressed much higher quantity than other EBV-latency transcripts. We describe...
Abstract Many agents are active in multiple myeloma, but the majority of patients relapse. This clinical pattern suggests most cancer cells eliminated, with clonogenic potential to mediate tumor regrowth relatively chemoresistant. Our previous data suggested that CD138+ myeloma plasma cannot undergo long-term proliferation rather arise from CD138neg B cells. We compared relative sensitivity these distinct cell types antimyeloma and found dexamethasone, lenadilomide, bortezomib,...
Hodgkin's disease (HD) has long been suspected to have an infectious precursor, and indirect evidence implicated Epstein-Barr virus (EBV), a ubiquitous herpesvirus, as causal agent. Recent molecular studies using EBER in situ hybridization or latency membrane protein-I (LMP-I) immunohistochemistry identified EBV latent infection up 50% of HD tumors. However, the epidemiologic features these cases not examined detail. To explore epidemiology EBV-positive so understand role etiology more...
We studied 25 patients with acute nonlymphocytic leukemia in second remission (20 patients) or third (5 whom autologous bone marrow transplantation was performed use of incubated ex vivo the alkylating agent 4-hydroperoxycyclophosphamide. Patients received intensive cytoreductive therapy busulfan and cyclophosphamide total body irradiation, followed by an infusion that had been collected remission, treated 4-hydroperoxycyclophosphamide, cryopreserved. Four died from bacterial fungal sepsis...
We conducted a placebo-controlled, double-blind study of acyclovir therapy for acute herpes zoster in immunocompromised patients. Of the 94 patients enrolled study, 52 had localized skin lesions at entry, and 42 disseminated cutaneous zoster. A one-week course intravenous (1500 mg per square meter body-surface area day) halted progression both groups, as determined by development or dissemination, visceral zoster, proportion cases deemed treatment failures. Significantly fewer treated with...
Although some reports have found an association between increasing HLA disparity donor and recipient fewer relapses after allogeneic blood or marrow transplantation (BMT), this potential benefit has been offset by more graft-versus-host disease (GVHD) nonrelapse mortality (NRM). However, the type of GVHD prophylaxis might influence balance toxicity relapse. The present study analyzed impact greater on outcomes a specific platform for nonmyeloablative (NMA), HLA-haploidentical...
Purpose Recent advances in nonmyeloablative (NMA), related HLA-haploidentical blood or marrow transplantation (haplo-BMT) have expanded the donor pool. This study evaluated effect of age on NMA haplo-BMT outcomes patients 50 to 75 years. Patients and Methods A retrospective analysis was performed 271 consecutive with hematologic malignancies, years, who received NMA, T-cell–replete high-dose post-transplantation cyclophosphamide. Results The median 61 115 (42%) 59, 129 (48%) 60 69, 27 (10%)...
Composite endpoints that not only encompass mortality and relapse, but other critical post-transplant events such as graft-versus-host disease, are being increasingly utilized to quantify survival without significant morbidity after allogeneic blood or marrow transplantation. High-dose, post-transplantation cyclophosphamide reduces severe disease with transplantation, making composite this management particularly interesting. We retrospectively analyzed 684 adults hematologic malignancies...
Hematopoietic cell transplantation (HCT) is curative for hematologic disorders, but outcomes are historically inferior when using HLA-mismatched donors. Despite unrelated donor registries listing > 38 million volunteers, 25%-80% of US patients lack an HLA-matched donor, with significant disparity across ethnic groups. We hypothesized that HCT a mismatched (MMUD) post-transplant cyclophosphamide (PTCy), novel strategy successful in overcoming genetic related donors, would be feasible and...
Hemorrhagic cystitis (HC) after bone marrow transplantation (BMT) has been ascribed to cyclophosphamide metabolites. HC also associated with excretion of the BK type polyomavirus. The relative contributions metabolites and virus in development following BMT are unknown.We conducted a randomized trial compare mesna forced diuresis for prophylaxis against 147 recipients. We studied association 95 consecutive recipients by prospectively monitoring urinary using polymerase chain reaction...
This study investigated the efficacy, toxicity, and pharmacokinetic interactions resulting from simultaneous combination chemotherapy highly active antiretroviral therapy (HAART) for patients with human immunodeficiency virus (HIV)-associated non-Hodgkin's lymphoma (NHL). In addition, effects on viral load, CD4 counts, opportunistic infections were examined use of combined HAART.Sixty-five previously untreated measurable disease at any stage HIV-associated NHL intermediate or high grade...
Abstract EBV DNA has been detected by Southern blot hybridization in 20‐25% of Hodgkin's disease tumor specimens and localized to the Reed‐Sternberg cells situ hybridization. In present investigation we used a 3 H‐labelled EBER I anti‐sense RNA for archival formalinfixed paraffin‐embedded previ ously shown Blot be EBV‐positive. 6 8 neoplastic showed an intense signal virtually all cells. The background lym phocytes, eosinophils, plasma histiocytes did not demonstrate significant each case...
We compared the outcome of nonmyeloablative allogeneic hematopoietic cell transplantation (HCT) for patients with relapsed or refractory Hodgkin lymphoma (HL) based on donor source. Ninety HL were treated conditioning followed by HCT from HLA-matched related, n=38, unrelated, n=24, HLA-haploidentical n=28 donors. Patients heavily pretreated a median 5 regimens and most had failed autologous (92%) local radiation therapy (83%). With follow-up 25 months, 2-year overall survivals,...
A total of 151 unselected malignant and nonmalignant lymphoid tissue samples were surveyed by Southern blotting for the presence Epstein-Barr virus (EBV) DNA. Eight 28 Hodgkin's disease (HD) (29%) had detectable EBV Both nodular sclerosis mixed cellularity histologic results positive. The tumor type with next highest frequency, 8%, was diffuse large cell lymphoma. DNA in some HD biopsies suggests that may be a factor pathogenesis this disease. Alternatively, its secondary to immune...
ABSTRACT Kaposi’s sarcoma (KS)-associated herpesvirus or human 8 (HHV8) DNA is found consistently in nearly all classical, endemic, transplant, and AIDS-associated KS lesions, as well several lymphomas. We have previously sequenced the genes for highly variable open reading frame K1 (ORF-K1) protein from more than 60 different HHV8 samples demonstrated that they display up to 30% amino acid variability cluster into four very distinct evolutionary subgroups (the A, B, C, D subtypes) correlate...
The CpG island of GADD45G was identified as a target sequence during the identification hypermethylated genes using methylation-sensitive representational difference analysis combined with 5-aza-2'-deoxycytidine demethylation. Located at commonly deleted region 9q22, is member DNA damage-inducible gene family. In response to stress shock, inhibits cell growth and induces apoptosis. Same other GADD45 members, ubiquitously expressed in all normal adult fetal tissues. However, its...
The Epstein-Barr virus (EBV)-encoded latency product EBNA-1 is functionally pleiotropic, being required for replication of the episomal form EBV genome and having a role in regulation transcription. direct DNA-binding protein, both transactivation are dependent on interaction with its cognate DNA recognition sequences. To better understand function, we have further characterized domain examined contributions other domains protein to activity. A Bal31 deletional analysis carboxy-terminal...