A5025812724- Infectious Diseases and Tuberculosis
- Orthopedic Infections and Treatments
- Tuberculosis Research and Epidemiology
- COVID-19 Clinical Research Studies
- Medical Imaging Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Infective Endocarditis Diagnosis and Management
- Bacterial Infections and Vaccines
- Bacterial Identification and Susceptibility Testing
- interferon and immune responses
- COVID-19 epidemiological studies
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Clostridium difficile and Clostridium perfringens research
- Cancer Research and Treatments
- Amoebic Infections and Treatments
- Immune cells in cancer
- Streptococcal Infections and Treatments
- Radiopharmaceutical Chemistry and Applications
- Antimicrobial Peptides and Activities
- Cancer therapeutics and mechanisms
- Immune Response and Inflammation
- Renal cell carcinoma treatment
- Pharmacological Receptor Mechanisms and Effects
- COVID-19 diagnosis using AI
Johns Hopkins University
2019-2024
Johns Hopkins Medicine
2019-2024
Pediatrics and Genetics
2022
Men experience more severe outcomes from coronavirus disease 2019 (COVID-19) than women. Golden Syrian hamsters were used to explore sex differences in the pathogenesis of a human clinical isolate acute respiratory syndrome 2 (SARS-CoV-2).
18 F-FDS PET/CT can detect and localize infections due to Enterobacterales in patients noninvasively monitor response antibiotic treatment.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein binds angiotensin-converting enzyme as its primary infection mechanism. Interactions between S and endogenous proteins occur after but are not well understood. We profiled binding of against >9000 human found an interaction estrogen receptor α (ERα). Using bioinformatics, supercomputing, experimental assays, we identified a highly conserved functional nuclear coregulator (NRC) LXD-like motif on the S2...
Pretomanid is a nitroimidazole antimicrobial active against drug-resistant Mycobacterium tuberculosis and approved in combination with bedaquiline linezolid (BPaL) to treat multidrug-resistant (MDR) pulmonary (TB). However, the penetration of these antibiotics into central nervous system (CNS), efficacy BPaL regimen for TB meningitis, are not well established. Importantly, there lack efficacious treatments meningitis due MDR strains, resulting high mortality. We have developed new methods...
Tuberculous meningitis (TB meningitis) is the most severe form of tuberculosis (TB), requiring 12 months multidrug treatment for cure, and associated with high morbidity mortality. High-dose rifampin (35 mg/kg/d) safe improves bactericidal activity standard-dose (10 rifampin-containing TB regimen in pulmonary TB. However, there are conflicting clinical data regarding its benefit meningitis, where outcomes may also be intracerebral inflammation. We conducted cross-species studies mice...
Tools for noninvasive detection of bacterial pathogens are needed but not currently available clinical use. We have previously shown that para-aminobenzoic acid (PABA) rapidly accumulates in a wide range pathogenic bacteria, motivating the development related PET radiotracers. In this study, 11C-PABA imaging was used to accurately detect and monitor infections due pyogenic bacteria multiple clinically relevant animal models. selectively detected muscle, intervertebral discs,...
Staphylococcus aureus is a major human pathogen causing serious implant–associated infections. Combination treatment with rifampin (10 to 15 mg/kg per day), which has dose-dependent activity, recommended treat S. orthopedic Rifampin, however, limited bone penetration. Here, dynamic 11C-rifampin positron emission tomography (PET) performed in prospectively enrolled patients confirmed infection (n = 3) or without 12) demonstrated bone/plasma area under the concentration-time curve ratio of...
Oxazolidinones are a novel class of antibacterials with excellent activity against resistant Gram-positive bacteria including strains causing multidrug-resistant tuberculosis (TB). Despite their efficacy, optimal dosing strategies to limit toxicities still under development. Here, we developed synthetic strategy for fluorine-18-radiolabeled oxazolidinones. As proof-of-concept, performed whole-body 18F-linezolid positron emission tomography (PET) in mouse model pulmonary TB noninvasive situ...
Exposing C-section infants to the maternal vaginal microbiome, coined "vaginal seeding", partially restores microbial colonization. However, whether seeding decreases metabolic disease risk is unknown. Therefore, we assessed effect of human on adiposity in a murine model. Germ-free mice were colonized with transitional stool from who received or control (placebo) double-blind randomized trial. There was reduction intraabdominal adipose tissue (IAAT) volume male that vaginally seeded compared...
Post-surgical implant-associated spinal infection is a devastating complication commonly caused by Staphylococcus aureus Biofilm formation thought to reduce penetration of antibiotics and immune cells, contributing chronic difficult-to-treat infections. A rabbit model posterior-approach surgery was created, in which bilateral titanium pedicle screws were interconnected plate at the level lumbar vertebra L6 inoculated with methicillin-resistant S.aureus (MRSA) bioluminescent strain. In vivo...
BACKGROUNDWhile most children who contract COVID-19 experience mild disease, high-risk with underlying conditions may develop severe requiring interventions. Kinetics of antibodies transferred via convalescent plasma early in disease have not been characterized.METHODSIn this study, were prospectively enrolled to receive high-titer (>1:320 anti-spike IgG; Euroimmun). Passive transfer and endogenous antibody production serially evaluated for up 2 months after transfusion. Commercial research...
Necrosis of lung lesions is a hallmark tuberculosis (TB) that promotes bacterial growth, dissemination, and transmission. This process driven by the persistent hyperactivation integrated stress response (ISR) pathway.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein binds angiotensin-converting enzyme (ACE2) at the cell surface, which constitutes primary mechanism driving SARS-CoV-2 infection. Molecular interactions between transduced S and endogenous proteins likely occur post-infection, but such are not well understood. We used an unbiased screen to profile binding of full-length against >9,000 human found significant S-host interactions, including one estrogen receptor...
<i>para-</i>Aminobenzoic acid (PABA) has been previously used as an exogenous marker to verify completion of 24-h urine sampling. Therefore, we hypothesized that PABA radiolabeled with <sup>11</sup>C might allow high-quality dynamic PET the kidneys less radiation exposure than other agents because its shorter biologic and physical half-life. We evaluated if <sup>11</sup>C-PABA can visualize renal anatomy quantify function in healthy rats rabbits a first-in-humans study on volunteers....
Abstract In the ongoing coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2), more outcomes are reported in males compared with females, including hospitalizations and deaths. Animal models can provide an opportunity to mechanistically interrogate causes of sex differences pathogenesis SARS-CoV-2. Adult male female golden Syrian hamsters (8-10 weeks age) were inoculated intranasally 10 5 TCID 50 SARS-CoV-2/USA-WA1/2020 euthanized at several...