A5015161123- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- COVID-19 Impact on Reproduction
- MicroRNA in disease regulation
- Bacterial Infections and Vaccines
- Pregnancy and Medication Impact
- COVID-19 diagnosis using AI
- Asthma and respiratory diseases
- Viral Infections and Vectors
- Circadian rhythm and melatonin
- Hormonal and reproductive studies
- Veterinary Oncology Research
- Nanoparticle-Based Drug Delivery
- Respiratory viral infections research
- Tumors and Oncological Cases
- Organ Transplantation Techniques and Outcomes
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- HIV-related health complications and treatments
- Parvovirus B19 Infection Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Immunotherapy and Biomarkers
University of Pennsylvania
2025
Johns Hopkins Medicine
2018-2024
Johns Hopkins University
2018-2024
Michigan State University
2017
Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage requirements, need...
Purpose: Enhancing immune responses in triple negative breast cancers (TNBCs) remains a challenge. Our study aimed to determine whether magnetic iron oxide nanoparticle (MION) hyperthermia (HT) can enhance abscopal effects with radiotherapy (RT) and checkpoint inhibitors (IT) metastatic TNBC model.Methods: One week after implanting 4T1-luc cells into the mammary glands of BALB/c mice, tumors were treated RT (3 × 8 Gy)±local HT, mild (HTM, 43 °C/20 min) or partially ablative (HTAbl, 45 °C/5...
Circulating androgens can modulate immune cell activity, but the impact of on viral pathogenesis remains unclear. Previous data demonstrate that testosterone reduces severity influenza A virus (IAV) infection in male mice by mitigating pulmonary inflammation rather than affecting replication. To examine responses mediated to mitigate IAV-induced inflammation, adult remained gonadally intact or were gonadectomized and treated with either placebo androgen-filled (i.e., dihydrotestosterone)...
Canine extramedullary plasma cell tumors (EMPs) most commonly arise in the skin, oral cavity, rectum, and colon. This retrospective study describes histopathological immunohistochemical characteristics associated clinical signs outcomes of laryngeal tracheal EMPs dogs. Five 5 were diagnosed at Penn Vet Diagnostic Laboratory. Clinical information was obtained via submission forms follow-up questionnaires. All dogs male (9 castrated), 7 to 15 years old, different breeds. Neoplasms composed...
Abstract Adeno‐associated virus (AAV) vector has shown multiple clinical breakthroughs, but its implementation in inhaled gene therapy remains elusive due to difficulty transducing lung airway cells. We demonstrate here AAV serotype 6 (AAV6) associated with extracellular vesicles (EVs) and secreted from vector‐producing HEK‐293 cells during preparation (EVAAV6) as a safe highly efficacious delivery platform for applications. Specifically, we discovered that EVAAV6 provided markedly enhanced...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has evolved into multiple variants. Animal models are important to understand variant pathogenesis, particularly for variants with mutations that have significant phenotypic or epidemiological effects. Here, cohorts naive previously infected Syrian hamsters (Mesocricetus auratus) were investigate viral pathogenesis and disease protection. Naive SARS-CoV-2 had consistent clinical outcomes, tissue...
ABSTRACT Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage...
SARS-CoV-2 infection during pregnancy is associated with severe COVID-19 and adverse fetal outcomes, but the underlying mechanisms remain poorly understood. Moreover, clinical studies assessing therapeutics against in are limited. To address these gaps, we developed a mouse model of pregnancy. Outbred CD1 mice were infected at embryonic day (E) 6, E10, or E16 adapted (maSCV2) virus. Outcomes gestational age-dependent, greater morbidity, reduced anti-viral immunity, viral titers, impaired...
Cervicovaginal secretions, or their components collected, are referred to as cervicovaginal lavage (CVL). CVL constituents have utility biomarkers and play protective roles in wound healing against HIV-1 infection. However, several of fluids less well understood, such extracellular RNAs carriers, for example, vesicles (EVs). EVs comprise a wide array double-leaflet membrane particles range diameter from 30 nm over one micron. The aim this study was determine whether differentially regulated...
Current influenza vaccines, live attenuated or inactivated, do not protect against antigenically novel A viruses (IAVs) of pandemic potential, which has driven interest in the development universal vaccines. Universal vaccine candidates targeting highly conserved antigens IAV nucleoprotein (NP) are promising as vaccines that induce T cell immunity, but concerns have been raised about safety inducing robust CD8 responses lungs. Using a mouse model, we systematically evaluated effects...
Background The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has had unprecedented effects on society and modern healthcare. In liver transplantation, uncertainty regarding safety of performing transplants during early stage pandemic resulted in increased waitlist mortality. Additionally, concerns about disease transmission led to avoidance deceased donors with infections. Several successful case reports describing incidental transplant organs from infections or intentional such into...
Abstract To catalyze SARS-CoV-2 research including development of novel interventive and preventive strategies, we characterized progression disease in depth a robust COVID-19 animal model. In this model, male female golden Syrian hamsters were inoculated intranasally with USA-WA1/2020. Groups mock-inoculated uninfected control animals euthanized at day 2, 4, 7, 14, 28 days post-inoculation to track multiple clinical, pathology, virology, immunology outcomes. SARS-CoV-2-inoculated...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has evolved into multiple variants. Animal models are important to understand variant pathogenesis, particularly for those with mutations that have significant phenotypic or epidemiological effects. Here, cohorts naïve previously infected Syrian hamsters ( Mesocricetus auratus ) were variants investigate viral pathogenesis and disease protection. Naïve SARS-CoV-2 had consistent clinical...
SARS-CoV-2 infection during pregnancy is associated with severe COVID-19 and adverse fetal outcomes, but the underlying mechanisms remain poorly understood. Moreover, clinical studies assessing therapeutics against in are limited. To address these gaps, we developed a mouse model of pregnancy. Outbred CD1 mice were infected at embryonic day (E) 6, E10, or E16 adapted (maSCV2) virus. Outcomes gestational age-dependent, greater morbidity, reduced anti-viral immunity, viral titers, more...
Abstract The goal of this study was to characterize extracellular vesicles (EVs) and miRNAs primate cervicovaginal lavage (CVL) during the menstrual cycle simian immunodeficiency virus (SIV) infection, determine if differentially regulated CVL might influence retrovirus replication. peripheral blood were collected from SIV-infected uninfected macaques. EVs enriched by stepped ultracentrifugation characterized thoroughly. miRNA profiles assessed with a medium-throughput stem-loop/hydrolysis...