A5002390547- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Radiomics and Machine Learning in Medical Imaging
- COVID-19 Clinical Research Studies
- Medical Imaging Techniques and Applications
- Medical Imaging and Pathology Studies
- Transplantation: Methods and Outcomes
- Advanced X-ray Imaging Techniques
- Respiratory Support and Mechanisms
- Pulmonary Hypertension Research and Treatments
- Immune Cell Function and Interaction
- Long-Term Effects of COVID-19
- Extracellular vesicles in disease
- Advanced X-ray and CT Imaging
- Mycobacterium research and diagnosis
- Photoacoustic and Ultrasonic Imaging
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Lung Cancer Diagnosis and Treatment
- Immune cells in cancer
- Tissue Engineering and Regenerative Medicine
- Optical Imaging and Spectroscopy Techniques
- MicroRNA in disease regulation
- Respiratory viral infections research
- Influenza Virus Research Studies
RWTH Aachen University
2023-2025
Universitätsklinikum Aachen
2024-2025
German Center for Lung Research
2014-2024
Medizinische Hochschule Hannover
2015-2024
Biomedical Research Institute
2023
European Molecular Biology Organization
2003-2008
European Bioinformatics Institute
2008
European Molecular Biology Laboratory
2007
Interleukin-3 (IL-3) is capable of supporting the proliferation a broad range hematopoietic cell types, whereas granulocyte colonystimulating factor (G-CSF) and macrophage CSF (M-CSF) represent critical cytokines in myeloid differentiation.When this was investigated pluripotent-stem-cell-based differentiation model, IL-3/G-CSF or IL-3/M-CSF exposure resulted continuous generation cells from an intermediate myeloid-cell-forming complex containing CD34 + clonogenic progenitor for more than 2...
Abstract Imaging intact human organs from the organ to cellular scale in three dimensions is a goal of biomedical imaging. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using European Synchrotron Radiation Facility (ESRF)’s Extremely Brilliant Source (EBS). The spatial coherence ESRF-EBS combined with our beamline equipment, sample preparation and scanning developments enabled us perform non-destructive,...
We recently showed that mucus from patients with ulcerative colitis, a chronic inflammatory disorder of the colon, is characterized by low level phosphatidylcholine (PC) while clinical studies reveal therapeutic addition PC using slow release preparations beneficial. The positive role in this disease still elusive. Here we tested hypothesis exogenous application has anti-inflammatory properties three model systems. First, human Caco-2 cells were treated tumor necrosis factor-α (TNF-α) to...
Abstract The increasing number of severe infections with multi-drug-resistant pathogens worldwide highlights the need for alternative treatment options. Given pivotal role phagocytes and especially alveolar macrophages in pulmonary immunity, we introduce a new, cell-based strategy to target bacterial airway infections. Here show that mass production therapeutic from induced pluripotent stem cells (iPSC) industry-compatible, stirred-tank bioreactors is feasible. Bioreactor-derived...
The SARS-CoV-2 coronavirus has led to a pandemic with millions of people affected. present study finds that risk-factors for severe COVID-19 disease courses, i.e. male sex, older age and sedentary life style are associated higher prostaglandin E2 (PGE2) serum levels in blood samples from unaffected subjects. In patients, PGE2 markedly elevated correlate positively severity. induces generation secretion infected lung epithelial cells by upregulating cyclo-oxygenase (COX)-2 reducing the...
Abstract COVID-19 survivors often suffer from post-acute sequelae of SARS-CoV-2 infection (PASC). Current evidence suggests dysregulated alveolar regeneration as a possible explanation for respiratory PASC, which deserves further investigation in suitable animal model. This study investigates morphological, phenotypical and transcriptomic features infected Syrian golden hamsters. We demonstrate that CK8 + differentiation intermediate (ADI) cells occur following SARS-CoV-2-induced diffuse...
Despite advancements in antifibrotic therapy, idiopathic pulmonary fibrosis (IPF) remains a medical condition with unmet needs. Single-cell RNA sequencing (scRNA-seq) has enhanced our understanding of IPF but lacks the cellular tissue context and gene expression localization that spatial transcriptomics provides. To bridge this gap, we profiled control patient lung using transcriptomics, integrating data an scRNA-seq atlas. We identified three disease-associated niches unique compositions...
The coordinated action of a variety virulence factors allows Salmonella enterica to invade epithelial cells and penetrate the mucosal barrier. influence age-dependent maturation barrier for microbial pathogenesis has not been investigated. Here, we analyzed infection neonate mice after oral administration. In contrast situation in adult animals, observed spontaneous colonization, massive invasion enteroabsorptive cells, intraepithelial proliferation formation large microcolonies. Mucosal...
Recurrent tumors (RT) of head and neck squamous cell carcinoma (HNSCC) occur in up to 60%, with poor therapeutic response detrimental prognosis. We hypothesized that HNSCC RTs successfully evade antitumor immune aimed reveal tumor microenvironment (TIME) changes primary (PT) corresponding RTs.Tumor-infiltrating leukocytes (TIL) 300 PTs 108 from two large independent clinically well-characterized cohorts [discovery cohort (DC), validation (VD)] were compared by IHC. mRNA expression analysis...
Abstract: High expression of SERPINA1 gene encoding acute phase protein, alpha1-antitrypsin (AAT), is associated with various tumors. We sought to examine the significance and AAT protein in non-small-cell lung cancer (NSCLC) patients NSCLC cell lines. Tumor adjacent non-tumor tissues serum samples from 351 were analyzed for levels. also studied impact on H1975 H661 behavior, vitro. Lower tumor but higher (n = 351, p 0.016) as well levels 170, 0.033) worse survival rates. Specifically, stage...
Enteropathogenic Escherichia coli (EPEC) represents a major causative agent of infant diarrhea associated with significant morbidity and mortality in developing countries. Although studied extensively vitro, the investigation host-pathogen interaction vivo has been hampered by lack suitable small animal model. Using RT-PCR global transcriptome analysis, high throughput 16S rDNA sequencing as well immunofluorescence electron microscopy, we characterize EPEC-host following oral challenge...
Disparities at the major histocompatibility complex (MHC) antigens and associated minor trigger harmful immune responses, leading to graft rejection after transplantation. We showed that MHC-silenced cells tissues are efficiently protected against rejection. In vascularized organs, endothelium is interface between donor recipient. This study therefore aimed reduce immunogenicity of lung by silencing MHC expression on endothelium. porcine lungs, short-hairpin RNAs targeting...
We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed Covid-19. Based first proof-of-concept study, we propose tomography as tool unravel pathophysiology Covid-19, extending conventional by third dimension allowing full quantification remodeling. By combining parallel cone beam geometry, autopsy samples...
Background and Aims: Metabolic dysfunction-associated steatohepatitis (MASH) is associated with c-Jun N-terminal kinases (JNK) activation across various cell types, but its hepatocyte-specific function in steatotic liver disease remains unclear. Our study investigates the role of JNK1/JNK2 during MASH progression dissect function. Approach Results: We showed that UK biobank patients a predicted-loss-of-function (pLOF) variant JNK1 presented an increased prevalence MASLD damage. Analysis...
Abstract Background Modeling of metabolic networks includes tasks such as network assembly, overview, calculation fluxes and testing the robustness network. Results YANAsquare provides a software framework for rapid assembly (flexible pathway browser with local or remote operation mode), overview (visualization routine editor) performance analysis (calculation flux modes well target tests). comes an easy-to-setup program package in Java. It is fully compatible integrates programs YANA...