A5014648398- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Medical Imaging and Pathology Studies
- Pulmonary Hypertension Research and Treatments
- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Neonatal Respiratory Health Research
- Sarcoidosis and Beryllium Toxicity Research
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Hernia repair and management
- Surgical Sutures and Adhesives
- COVID-19 Clinical Research Studies
- Intestinal and Peritoneal Adhesions
- Cardiovascular Function and Risk Factors
- Organ and Tissue Transplantation Research
- Long-Term Effects of COVID-19
- Mechanical Circulatory Support Devices
- Renal Transplantation Outcomes and Treatments
- Occupational and environmental lung diseases
- Bacterial Infections and Vaccines
- Respiratory Support and Mechanisms
- Antibiotic Resistance in Bacteria
- CAR-T cell therapy research
- MicroRNA in disease regulation
- Body Contouring and Surgery
Medizinische Hochschule Hannover
2019-2025
German Center for Lung Research
2019-2025
Philadelphia University
2014
Pulmonary arterial hypertension (PAH) is a devastating disease with poor prognosis and limited therapeutic options. We screened for pathways that may be responsible the abnormal phenotype of pulmonary smooth muscle cells (PASMCs), major contributor PAH pathobiology, identified cyclin-dependent kinases (CDKs) as overactivated in specimens derived from patients idiopathic PAH. This increased CDK activity confirmed at level mRNA protein expression human experimental PAH, respectively. Specific...
Despite advancements in antifibrotic therapy, idiopathic pulmonary fibrosis (IPF) remains a medical condition with unmet needs. Single-cell RNA sequencing (scRNA-seq) has enhanced our understanding of IPF but lacks the cellular tissue context and gene expression localization that spatial transcriptomics provides. To bridge this gap, we profiled control patient lung using transcriptomics, integrating data an scRNA-seq atlas. We identified three disease-associated niches unique compositions...
The pathogenetic role of angiogenesis in interstitial lung diseases (ILDs) is controversial. This study represents the first investigation spatial complexity and molecular motifs microvascular architecture important subsets human ILD. aim our was to identify specific variants neoangiogenesis three common pulmonary injury patterns We performed comprehensive compartment-specific analysis 24 explants with usual intersitial pneumonia (UIP), nonspecific (NSIP) alveolar fibroelastosis (AFE) using...
A wide range of cardiac symptoms have been observed in COVID-19 patients, often significantly influencing the clinical outcome. While pathophysiology pulmonary manifestation has substantially unraveled, underlying pathomechanisms involvement are largely unknown. In this multicentre study, we performed a comprehensive analysis heart samples from 24 autopsies with confirmed SARS-CoV-2 infection and compared them to age-matched Influenza H1N1 (n = 16), lymphocytic non-influenza myocarditis...
(1) Background: HNSCC is a highly heterogeneous and relapse-prone form of cancer. We aimed to expand the immunological tool kit against by conducting functional screen generate chimeric antigen receptor (CAR)-NK-92 cells that target HER1/epidermal growth factor (EGFR). (2) Methods: Selected CAR-NK-92 cell candidates were tested for enhanced reduction cells, CD107a expression IFNγ secretion in different co-culture models. For representative models, patient-derived primary (pHNSCC) lines...
(1) Background: In COVID-19 survivors there is an increased prevalence of pulmonary fibrosis which the underlying molecular mechanisms are poorly understood; (2) Methods: this multicentric study, n = 12 patients who succumbed to due progressive respiratory failure were assigned early and late group (death within ≤7 >7 days hospitalization, respectively) compared 11 healthy controls; mRNA protein expression as well biological pathway analysis performed gain insights into evolution...
Background: Fibroblastic foci (FF) are characteristic features of usual interstitial pneumonia (UIP)/idiopathic pulmonary fibrosis (IPF) and one cardinal feature thought to represent a key mechanism pathogenesis. Hence, FF have high impact on UIP/IPF diagnosis in current guidelines. However, although less frequent, these histomorphological hallmarks also occur other fibrotic diseases. Currently, there is therefore gap knowledge regarding the underlying molecular similarities differences...
Background and Objectives:The rat Optimized Peritoneal Adhesion Model (OPAM) was developed to provoke adhesion formation with high reproducibility in incidence extent.In a recent study, the starch-based hemostats 4DryField PH Arista AH were tested for their capabilities prevent formation, former one certified prevention hemostasis, latter only hemostasis.As two further hemostats, i.e., HaemoCer PLUS StarSil, have officially been meantime, present study conducted examine efficacy.Materials...
Abstract Background Sarcoidosis is a disease of unknown etiology characterized by the formation immune cell accumulation (granuloma) in lung and other tissues. Chronic sarcoidosis may lead to pulmonary fibrosis. Aim To unravel cellular niches within granuloma chronic patients using spatial transcriptomics. Methods Spatial transcriptomics Visium platform (10x Genomics) was performed on nine granuloma-containing explants from patients. Validation gene expression through immunohistofluorescence...
We aimed to evaluate the feasibility of [18F]flurpiridaz PET/CT for localization parathyroid adenomas in patients with primary hyperparathyroidism (pHPT). Methods: Data 11 pHPT undergoing dual-time-point hyperfunctioning glands were retrospectively analyzed. findings compared results other imaging tests, laboratory parameters, intraoperative findings, and final histology, serving as reference standard. Results: identified 10 (91%) studied. Parathyroid exclusively visualized early PET images....
Unlike the vast amount of animal data available on recellularization allogenic decellularized heart valves (DHVs), there have only been sporadic histologic reports such grafts in humans.Two experienced cardiac pathologists independently evaluated human specimens obtained during reoperation between December 2010 and April 2017 DHVs seven categories after automated staining (scores: 0 to 6) comparison with published data. An optimal result 42 points was classified as 100%.We found that 364...
Abstract Primary liver cancer (PLC) comprising hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) represents the third deadliest worldwide with still insufficient treatment options. We have previously found that CD4 T helper 1 (Th1) response is indispensable for protection against PLC. In present research, we aimed to test potent inducers of Th1 responses, live-attenuated Listeria monocytogenes ∆actA/∆inlB strain as preventive/therapeutic vaccine candidate in fibrosis, HCC, CCA....
Abstract Objectives. As the central organ of respiratory system, human lung is responsible for supplying oxygen to blood, which reaches erythrocytes by diffusion through alveolar walls and then distributed throughout body. By exploiting difference in electron density detected a phase shift soft tissue, high-resolution x-ray phase-contrast computed tomography (XPCT) can resolve biological structures sub- μ m range, shedding new light on three-dimensional structure lungs, physiological...
The continuous emergence of multidrug-resistant bacterial pathogens poses a major global healthcare challenge, with Klebsiella pneumoniae being prominent threat. We conducted comprehensive study on K. pneumoniae's antibiotic resistance mechanisms, focusing outer membrane vesicles (OMVs) and polymyxin, last-resort antibiotic. Our research demonstrates that OMVs protect bacteria from polymyxins. derived Polymyxin B (PB)-stressed exhibited heightened protective efficacy due to increased...
Interstitial lung diseases encompass a large number of entities, which are characterised by small partially overlapping fibrosing injury patterns, either alone or in combination. Thus, the presently applied morphological diagnostic criteria do not reliably discriminate different interstitial diseases. We therefore analysed critical regulatory pathways and signalling molecules involved pulmonary remodelling with regard to their suitability. Using laser-microdissection microarray techniques,...
Hepatocellular carcinoma (HCC) is the most common primary malignancy of liver with a very poor prognosis and constantly growing incidence. Among other risks HCC, metabolic disorders obesity have been extensively investigated over recent decades. The latter can promote nonalcoholic fatty disease (NAFLD) leading to inflammatory form steatohepatitis (NASH), that, in turn, promotes HCC. Molecular determinants this pathogenic progression, however, remain largely undefined. In study, we focussed...
Abstract Idiopathic pulmonary fibrosis (IPF) is an irreversible, age-related diffuse parenchymal lung disease of poorly defined etiology. Many patients with IPF demonstrate distinctive lymphocytic interstitial infiltrations within remodeled tissue uncertain pathogenetic relevance. Histopathological examination explant revealed accentuated lymphoplasmacellular accumulations in close vicinity to, or even infiltrating, tissue. Similarly, we found significant B cells interfused T two murine...
Alveolar capillary dysplasia (ACD) is a rare lung developmental disorder leading to persistent pulmonary arterial hypertension and fatal outcomes in newborns. The current study analyzed the microvascular morphology underlying molecular background of ACD. One ACD group (n = 7), one 20), healthy con1trol 16) were generated. Samples histologically confirmed examined by exome sequencing array-based comparative genomic hybridization. Vascular was using scanning electron microscopy corrosion...