A5056573031- Advanced X-ray Imaging Techniques
- Advanced X-ray and CT Imaging
- Cell Image Analysis Techniques
- Pediatric Urology and Nephrology Studies
- Advanced MRI Techniques and Applications
- Interprofessional Education and Collaboration
- COVID-19 Clinical Research Studies
- Nuclear Physics and Applications
- Cardiovascular and Diving-Related Complications
- Photoacoustic and Ultrasonic Imaging
- Long-Term Effects of COVID-19
- Medical Imaging Techniques and Applications
- Single-cell and spatial transcriptomics
- Advanced Electron Microscopy Techniques and Applications
- Genetic and Kidney Cyst Diseases
- Lymphatic System and Diseases
- RNA Research and Splicing
- Nursing Roles and Practices
- MRI in cancer diagnosis
- Cerebrospinal fluid and hydrocephalus
- Thermal Regulation in Medicine
- Plant tissue culture and regeneration
- Retinal Imaging and Analysis
- Mathematical Biology Tumor Growth
- Traumatic Brain Injury and Neurovascular Disturbances
University College London
2017-2025
Sheffield Hallam University
2004-2024
European Synchrotron Radiation Facility
2024
Johannes Gutenberg University Mainz
2024
Université Grenoble Alpes
2024
RWTH Aachen University
2024
Laboratoire d’Imagerie Biomédicale
2022
Newcastle University
2020
Logan Hospital
2018
Metro South Health
2018
Abstract Imaging intact human organs from the organ to cellular scale in three dimensions is a goal of biomedical imaging. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using European Synchrotron Radiation Facility (ESRF)’s Extremely Brilliant Source (EBS). The spatial coherence ESRF-EBS combined with our beamline equipment, sample preparation and scanning developments enabled us perform non-destructive,...
Background Current clinical imaging modalities such as CT and MRI provide resolution adequate to diagnose cardiovascular diseases but cannot depict detailed structural features in the heart across length scales. Hierarchical phase-contrast tomography (HiP-CT) uses fourth-generation synchrotron sources with improved x-ray brilliance high energies micron-resolution of intact adult organs unprecedented detail. Purpose To evaluate capability HiP-CT macro- microanatomy structurally normal...
Abstract Previous studies have emphasized culturally sensitive curricula in the context of enhancing minoritized students’ education. We examined relationship between second-year higher education perceptions cultural sensitivity their curriculum and both majoritized interest course. A total 286 (228 F) students rated on six scales using a revised version Culturally Sensitive Curricula Scales (CSCS-R), perceived quality relationships with teachers, interest. The CSCS-R widened construct two...
Heterogeneity of lymphatic vessels during embryogenesis is critical for organ-specific function. Little known about lymphatics in the developing kidney, despite their established roles pathology mature organ. We performed three-dimensional imaging to characterize vessel formation mammalian embryonic kidney at single-cell resolution. In mouse, we visually and quantitatively assessed development vessels, remodeling from a ring-like anastomosis under nascent renal pelvis; site VEGF-C...
Disruption of retinal vasculature is linked to various diseases, including diabetic retinopathy and macular degeneration, leading vision loss. We present here a novel algorithmic approach that generates highly realistic digital models human blood vessels, based on established biophysical principles, fully-connected arterial venous trees with single inlet outlet. This approach, using physics-informed generative adversarial networks (PI-GAN), enables the segmentation reconstruction vessel no...
Structural changes to microvascular networks are increasingly highlighted as markers of pathogenesis in a wide range disease, e.g. Alzheimer's vascular dementia and tumour growth. This has motivated the development dedicated 3D imaging techniques, alongside creation computational modelling frameworks capable using reconstructed simulate functional behaviours such blood flow or transport processes. Extraction from data broadly consists two image processing steps: segmentation followed by...
Improving the scalability of tissue imaging throughput with bright, coherent X-rays requires identifying and mitigating artifacts resulting from interactions between matter. At synchrotron sources, long-term soft tissues in solution can result gas bubble formation or cavitation, which dramatically compromises image quality integrity samples. By combining in-line phase-contrast chromatography real time, we were able to track onset evolution high-energy X-ray-induced bubbles ethanol-embedded...
Background: Fibrotic lung disease is a progressive illness that causes scarring and ultimately respiratory failure, with irreversible damage by the time its diagnosed on computed tomography imaging. Recent research postulates role of vasculature pathogenesis disease, recent development high-resolution hierarchical phase contrast (HiP-CT), we have potential to understand detect changes in lungs long before conventional However, gain quantitative insight into vascular you first need be able...
Water security in the Global South is increasingly threatened by rapid socioeconomic changes, including urbanization, population growth, and expansion of irrigated agriculture. These dynamics not only intensify competition for limited water resources but also amplify vulnerability to climate change impacts. The Sudano-Sahelian region, Sokoto Rima Basin (study area) Nigeria, particularly sensitive these pressures, having experienced severe droughts 1970s 1980s. This study adopts a...
Therapies targeting blood vessels hold promise for autosomal dominant polycystic kidney disease (ADPKD), the commonest inherited disorder causing failure. However, onset and nature of vascular abnormalities in ADPKD are poorly defined. Accordingly, we employed a combination single-cell transcriptomics, three-dimensional imaging with geometric, topological fractal analyses, multimodal magnetic resonance arterial spin labelling to investigate aberrant microvasculature kidneys. Within human...
Abstract Technological advancements in X-ray imaging using bright and coherent synchrotron sources now allows the decoupling of sample size resolution while maintaining high sensitivity to microstructures soft, partially dehydrated tissues. The continuous developments multiscale resulted hierarchical phase-contrast tomography, a comprehensive approach address challenge organ-scale (up tens centimeters) soft tissue with down cellular level. Using this technique, we imaged ex vivo an entire...
Automated blood vessel segmentation is critical for biomedical image analysis, as morphology changes are associated with numerous pathologies. Still, precise difficult due to the com- plexity of vascular structures, anatomical variations across patients, scarcity annotated public datasets, and quality images. We present a thorough literature review, highlighting state machine learning techniques diverse organs. Our goal pro- vide foundation on topic identify robust baseline model application...
Abstract We present a 3D discrete‐continuum model to simulate blood pressure in large microvascular tissues the absence of known capillary network architecture. Our hybrid approach combines 1D Poiseuille flow description for large, discrete arteriolar and venular networks coupled continuum‐based Darcy model, point sources flux, transport bed. evaluate our using vascular imaged from mouse brain medulla/pons multi‐fluorescence high‐resolution episcopic microscopy (MF‐HREM). use fully‐resolved...
Abstract Efficient algorithms are needed to segment vasculature in new three-dimensional (3D) medical imaging datasets at scale for a wide range of research and clinical applications. Manual segmentation vessels images is time-consuming expensive. Computational approaches more scalable but have limitations accuracy. We organized global machine learning competition, engaging 1,401 participants, help develop deep methods 3D blood vessel segmentation. This paper presents detailed analysis the...
ABSTRACT Hallmarks of autosomal dominant polycystic kidney disease (ADPKD), the most common hereditary anomaly, include expanding fluid-filled epithelial cysts, inflammation, and fibrosis. Despite previous work showing potential vascular-based therapies, renal microvascular alterations in ADPKD, their timing, are poorly understood. Using single-cell transcriptomics human microvasculature, we identify a population endothelial cells adjacent to cysts ADPKD. This pericystic endothelium,...
Abstract Automated blood vessel segmentation is critical for biomedical image analysis, as morphology changes are associated with numerous pathologies. Still, precise difficult due to the complexity of vascular structures, anatomical variations across patients, scarcity annotated public datasets, and quality images. Our goal provide a foundation on topic identify robust baseline model application using new imaging modality, Hierarchical Phase-Contrast Tomography (HiP-CT). We begin an...