Daniele Tolomeo
A5090869061- Neuroscience and Neuropharmacology Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced MRI Techniques and Applications
- Alzheimer's disease research and treatments
- Advanced Neuroimaging Techniques and Applications
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- Electron Spin Resonance Studies
- Functional Brain Connectivity Studies
- Lanthanide and Transition Metal Complexes
- Neonatal and fetal brain pathology
- Health, Environment, Cognitive Aging
- Traumatic Brain Injury Research
- Prion Diseases and Protein Misfolding
- Epilepsy research and treatment
- Glioma Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Retinoids in leukemia and cellular processes
- Genetics and Neurodevelopmental Disorders
- Computational Drug Discovery Methods
- MRI in cancer diagnosis
- Genetic and Kidney Cyst Diseases
- Pharmacological Effects and Toxicity Studies
- Endoplasmic Reticulum Stress and Disease
University College London
2020-2025
Ixico (United Kingdom)
2025
Mario Negri Institute for Pharmacological Research
2013-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2022
Centro San Giovanni di Dio Fatebenefratelli
2012-2014
Abstract Background This study aimed to have international experts converge on a harmonized definition of whole hippocampus boundaries and segmentation procedures, define standard operating procedures for magnetic resonance (MR)‐based manual hippocampal segmentation. Methods The panel received questionnaire regarding procedures. Quantitative information was supplied allow evidence‐based answers. A recursive anonymous Delphi procedure used achieve convergence. Significance agreement among...
Abstract Aims The causes of distinct patterns reduced cortical thickness in the common human epilepsies, detectable on neuroimaging and with important clinical consequences, are unknown. We investigated underlying mechanisms thinning using a systems‐level analysis. Methods Imaging‐based structural maps from large‐scale epilepsy study were overlaid highly spatially resolved brain gene expression data Allen Human Brain Atlas. Cell‐type deconvolution, differential analysis cell‐type enrichment...
Despite an apparently silent imaging, some patients with mild traumatic brain injury (TBI) experience cognitive dysfunctions, which may persist chronically. Brain changes responsible for these dysfunctions are unclear and commonly overlooked. It is thus crucial to increase our understanding of the mechanisms linking initial event functional deficits, provide objective evidence tissue alterations underpinning deficits. We first set up a murine model closed-head controlled cortical impact,...
Traumatic brain injury is increasingly common in older individuals. Older age one of the strongest predictors for poor prognosis after trauma, a phenomenon driven by presence extra-cranial comorbidities as well pre-existent pathologies associated with cognitive impairment and volume loss (such cerebrovascular disease or age-related neurodegeneration). Furthermore, ageing dysregulated immune response, which includes attenuated responses to infection vaccination, failure resolve inflammation...
Abstract Glioblastoma (GBM) is the most common and aggressive brain tumour with stark resistance to available therapies, leading relapse a median survival of <15 months. A key cause therapy diffuse infiltration cells into regions surrounding tumour, which presents major clinical challenge as existing imaging techniques offer limited detection resectable margin. Here, we use diffusion weighted (DWI) apply multiple echo time neurite orientation dispersion density (MTE-NODDI) model tool...
Therapies targeting blood vessels hold promise for autosomal dominant polycystic kidney disease (ADPKD), the commonest inherited disorder causing failure. However, onset and nature of vascular abnormalities in ADPKD are poorly defined. Accordingly, we employed a combination single-cell transcriptomics, three-dimensional imaging with geometric, topological fractal analyses, multimodal magnetic resonance arterial spin labelling to investigate aberrant microvasculature kidneys. Within human...
Marinesco-Sjögren syndrome (MSS) is a rare, early onset, autosomal recessive multisystem disorder characterized by cerebellar ataxia, cataracts and myopathy. Most MSS cases are caused loss-of-function mutations in the gene encoding SIL1, nucleotide exchange factor for molecular chaperone BiP which essential correct protein folding endoplasmic reticulum. Woozy mice carrying spontaneous Sil1 mutation recapitulate key pathological features of MSS, including atrophy with degeneration Purkinje...
Glucose is the central nervous system's only energy source. Imaging techniques capable to detect pathological alterations of brain metabolism are useful in different diagnostic processes. Such also beneficial for assessing evaluation efficacy therapies pre-clinical and clinical stages diseases. Chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) a possible alternative positron emission tomography (PET) that has been widely explored cancer research humans animal...
Post-traumatic epilepsy (PTE) accounts for 5% of all epilepsies and 10-20% the acquired forms. The latency between traumatic brain injury (TBI) onset in high-risk patients offers a therapeutic window intervention to prevent or improve disease course. However, progress towards effective treatments has been hampered by lack sensitive prognostic biomarkers PTE, targets. There is therefore pressing clinical need preclinical PTE models suitable biomarker discovery drug testing. We characterized...
Alzheimer's disease (AD) is the most common form of dementia characterized by synaptic dysfunction, memory loss, neuroinflammation, and neuronal cell death. Amyloid-β (Aβ), recognized as main culprit AD, aggregates accumulates in extracellular compartment neuritic plaques, after deregulation its production or clearance. Apolipoprotein E (ApoE) plays a major role Aβ clearance expression transcriptionally regulated liver X receptor retinoid receptors (RXRs) system. Bexarotene (BEXA), an RXR...
ABSTRACT Hallmarks of autosomal dominant polycystic kidney disease (ADPKD), the most common hereditary anomaly, include expanding fluid-filled epithelial cysts, inflammation, and fibrosis. Despite previous work showing potential vascular-based therapies, renal microvascular alterations in ADPKD, their timing, are poorly understood. Using single-cell transcriptomics human microvasculature, we identify a population endothelial cells adjacent to cysts ADPKD. This pericystic endothelium,...
Abstract Task-free functional connectivity in animal models provides an experimental framework to examine phenomena under controlled conditions and allows comparison with invasive or terminal procedures. To date, acquisitions are performed varying protocols analyses that hamper result integration. We introduce StandardRat , a consensus rat MRI acquisition protocol tested across 20 centers. develop this optimized processing parameters, we initially aggregated 65 imaging datasets acquired rats...
Mild traumatic brain injury (mTBI) mostly causes transient symptoms, but repeated (r)mTBI can lead to neurodegenerative processes. Diagnostic tools evaluate the presence of ongoing occult neuropathology are lacking. In a mouse model rmTBI, we investigated MRI and plasma biomarkers damage before chronic functional impairment arose. Anesthetized adult male female C57BL/6J mice were subjected rmTBI or sham procedure. Sensorimotor deficits evaluated up 12 months post-injury in SNAP Neuroscore...
Abstract The common human epilepsies are associated with distinct patterns of reduced cortical thickness, detectable on neuroimaging, important clinical consequences. To explore underlying mechanisms, we layered MRI-based structural maps from a large-scale epilepsy neuroimaging study onto highly spatially-resolved brain gene expression data, identifying >2,500 genes overexpressed in regions compared to relatively-protected regions. resulting set differentially-expressed shows enrichment...
Chronic hypertension is a major risk factor for the development of neurodegenerative disease, yet etiology hypertension-driven neurodegeneration remains poorly understood. Forming unique interface between systemic circulation and brain, blood-cerebrospinal fluid barrier (BCSFB) at choroid plexus (CP) has been proposed as key site vulnerability to that may initiate downstream processes. However, our ability understand BCSFB’s role in pathological processes has, date, restricted by lack...
Traumatic brain injury (TBI) is a major cause of death and disability. Despite progress in neurosurgery critical care, patients still lack form neuroprotective treatment that can counteract or attenuate progression. Inflammation after TBI key modulator progression neurodegeneration, but its spatiotemporal dissemination only partially known. In vivo approaches to study post-traumatic inflammation longitudinally are pivotal for monitoring progression/recovery the effectiveness therapeutic...
Objective: To define a harmonized protocol for manual hippocampal segmentation from magnetic resonance scans. Background Heterogeneity of landmarks among protocols leads to different volume estimates, hampering comparison studies and clinical use. Landmark differences the 12 most common were extracted, operationalized, quantitatively investigated. The results presented Delphi panel, consisting seventeen researchers with substantial expertise in segmentation, reach an evidence-based consensus...
Alzheimer's Disease (AD) is the most common form of dementia in elderly people which causes memory loss and behavioral problems. This study has been conducted within IMI-PharmaCog consortium with objective to develop biomarkers sensitive disease progression drug treatment both human animal models. Here we present a longitudinal MRS investigate brain metabolic alterations characterizing AD. The experiments were on 7T Bruker Biospec 70/30 consisted two single voxel acquisition (dorsal...
Heterogeneity of landmarks among protocols leads to different volume estimates, hampering comparison studies and clinical use. There is an urgent need define a harmonized protocol for manual hippocampal segmentation from magnetic resonance scans. Landmark differences the 12 most common were extracted, operationalized, quantitatively investigated. The results presented Delphi panel, consisting seventeen researchers with substantial expertise in segmentation, order reach evidence-based...
Understanding the structural changes induced by Alzheimer's disease (AD) has a great relevance since it would allow identifying biomarkers suitable for an early diagnosis. Within IMI-PharmaCog consortium, we present longitudinal MRI study, followed histology, of two different transgenic mouse models to investigate degeneration associated β-amyloid cerebral deposition. We performed high resolution (146×117×146μm3 vo×el) in-vivo with 7T Bruker Biospec 70/30. from 4 26 months double (TASTPM)...