A5025548416- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- IL-33, ST2, and ILC Pathways
- Cystic Fibrosis Research Advances
- Asthma and respiratory diseases
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Immune Cell Function and Interaction
- Respiratory and Cough-Related Research
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Recycling and Waste Management Techniques
- Dysphagia Assessment and Management
- COVID-19 diagnosis using AI
- Pneumonia and Respiratory Infections
- Pleural and Pulmonary Diseases
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Biochemical and Structural Characterization
- Radiation Effects and Dosimetry
Fraunhofer Institute for Toxicology and Experimental Medicine
2016-2025
German Center for Lung Research
2015-2022
Medizinische Hochschule Hannover
2022
Harvard University
2020
Rockefeller University
2020
Yale University
2020
National Institutes of Health
2020
Abstract The increasing number of severe infections with multi-drug-resistant pathogens worldwide highlights the need for alternative treatment options. Given pivotal role phagocytes and especially alveolar macrophages in pulmonary immunity, we introduce a new, cell-based strategy to target bacterial airway infections. Here show that mass production therapeutic from induced pluripotent stem cells (iPSC) industry-compatible, stirred-tank bioreactors is feasible. Bioreactor-derived...
The pathogenesis of respiratory syncytial virus (RSV) bronchiolitis in infants remains poorly understood. Mouse models implicate pulmonary T cells the development RSV disease. cell responses are initiated by dendritic (DCs), which accumulate lungs RSV-infected mice. In with bronchiolitis, previous reports have shown that DCs mobilised to nasal mucosa, but data on lower airway DC lacking.To determine presence and phenotype associated immune bronchoalveolar lavage (BAL) peripheral blood...
In the context of cystic fibrosis, Pseudomonas aeruginosa biofilms often develop in vicinity airway mucus, which acts as a protective physical barrier to inhaled matter. However, mucus can also adsorb small drug molecules administered aerosols, including antibiotics, thereby reducing their bioavailability. The efficacy antibiotics is typically assessed by determining MIC using vitro assays. This widespread technique, however, does not consider either bacterial biofilm formation or influence...
Antibiotic resistance is a significant healthcare concern. Therefore, identifying target molecules that can serve as antibiotic substitutes crucial. Among the promising candidates are antimicrobial peptides (AMPs). AMPs defense mechanisms of innate immune system which exist in almost all living organisms. Research on some amphibians has shown that, addition to their effectiveness, also exhibit anti-inflammatory and anti-carcinogenic properties. In this study, we identify characterize...
Smokers with apparently “healthy” lungs suffer from more severe and frequent viral respiratory infections, but the mechanisms underlying this observation are still unclear. Epithelial cells dendritic (DC) form first line of defense against inhaled noxes such as smoke or viruses. We therefore aimed to obtain insight how cigarette affects DCs epithelial influences response infection. Female C57BL/6J mice were exposed for one hour daily 24 days then challenged i.n. mimic Toll-like receptor 3...
Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino- and influenza viruses at high concentrations. We sought to investigate whether ASA metabolite salicylic (SA) inhibit SARS-CoV-2 since it might use similar pathways viruses. The compound-treated cells were infected SARS-CoV-2. Viral replication was analysed by RTqPCR. compounds suppressed in cell culture a patient-near system using human precision-cut lung slices two orders of magnitude....
Human rhinovirus (RV) is a major risk factor for chronic obstructive pulmonary disease (COPD) and asthma exacerbations. The exploration of RV pathogenesis has been hampered by lack disease-relevant model systems. We performed detailed characterization host responses to infection in human lung tissue ex vivo investigated whether these are relevant patients with COPD asthma. In addition, impact the viral replication inhibitor rupintrivir was evaluated. precision-cut slices (PCLS) were infected...
Abstract Background Antiviral drugs such as rupintrivir may have an immune-modulatory effect in experimentally induced allergic asthma with subsequent RV infection. We infected lung slices of house-dust mite (HDM)-sensitized asthmatic mice ex vivo human rhinovirus (RV) and investigated the antiviral drug on RV-induced cytokine response tissue HDM-sensitized vivo. Methods Mice were sensitized HDM. Precision-cut (PCLS) prepared from or non-sensitized mice. Lung together rupintrivir. Modulation...
Abstract The peptide sequence KKIRVRLSA was synthesized in a dimeric structure (SET‐M33DIM) and evaluated as candidate drug for infections due to multidrug‐resistant (MDR) Gram‐negative pathogens. SET‐M33DIM showed significant antibacterial activity against MDR strains of Klebsiella pneumoniae, Acinetobacter baumannii , Escherichia coli (Minimal Inhibitory Concentration [MICs], 1.5‐11 µM), less Pseudomonas aeruginosa (MICs, 11‐22 µM). It very low toxicity vitro, ex vivo, vivo; cytotoxicity...
Abstract Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino- and influenza viruses at high concentrations. We sought to investigate whether ASA metabolite salicylic (SA) inhibit SARS-CoV-2 since it might use similar pathways viruses. The compound-treated cells were infected SARS-CoV-2. Viral replication was analyzed by RTqPCR. compounds suppressed in cell culture a patient-near system using human precision-cut lung slices two orders of...
Chronic respiratory infections with the gram-negative bacterium Pseudomonas aeruginosa are an important co-morbidity for quality of life and prognosis people cystic fibrosis (CF). Such long-term colonization, sometimes lasting up to several decades, represents a unique opportunity investigate pathogen adaptation processes host. Our studies aimed resolve if what extent bacterial CF airways influences fitness grow persist in lungs. Marker-free competitive experiments serial P. isolates...
Background Respiratory viruses such as respiratory syncytial virus (RSV) or rhinovirus are one of the major causes for tract infections causing common cold disease. viral range from mild symptoms in adults to serious illness especially very young elderly well patients suffering lung diseases being immunocompromised due other reasons. Engystol (EGY-2) is a multicomponent, multitarget preparation consisting Vincetoxicum hirundinaria and Sulfur various dilutions. The study objective was test...
Rhinovirus (RV) is the predominant virus causing respiratory tract infections. Bronchobini® a low dose multi component, target preparation used to treat inflammatory diseases such as common cold, described ease severity of symptoms cough and viscous mucus production. The aim study was assess efficacy in RV infection elucidate its mode action. Therefore, Bronchobini®’s ingredients (BRO) were assessed an ex vivo model using mouse precision-cut lung slices, organotypic tissue capable reflect...
<b><i>Objectives:</i></b> Our previous data demonstrated that allergic airway inflammation induces migration of dendritic cells (DC) into sensory jugular and nodose ganglia (jugular-nodose ganglion complex; JNC). Here we investigated the effects steroid treatment regarding expression DC calcitonin gene-related peptide (CGRP)-immunoreactive neurons vagal during inflammation. <b><i>Methods:</i></b> A house dust mite (HDM) model for was used. The...
Annual seasonal epidemics with Influenza A virus (IAV) strains result in 3-5 million severe cases children and elderly individuals. Therefore, this study the immune response of lower respiratory tract tissue to H1N1 (California/04/2009) pandemic strain was compared H3N2 (Perth/16/2009) viable human precision-cut lung slices (PCLS) ex vivo. PCLS were inoculated 104-106 ffu/mL for 1 h incubated 48 post infection. Local measured MSD, damage observed by LDH release
Millions of asthmatic patients suffer from virus induced exacerbation mainly caused by human rhinovirus (HRV). Insufficient elimination and inadequate immune response are assumed to be responsible for exacerbation. Therefore, in this study the impact an background on anti-viral pro-inflammatory was investigated precision-cut lung slices (PCLS) HDM sensitized mice. Balb/c mice were challenged with or saline i.n. 28 days. PCLS prepared infected HRV1B (105 TCID<sub>50</sub>/mL) UV-inactivated...
Cystic fibrosis patients are highly susceptible to <i>Pseudomonas aeruginosa</i> which rapidly adapts the lung microenvironment and causes chronic inflammation strongly contributing disease pathogenesis. In presented study, fresh human tissue was infected <i>ex vivo</i> with <i>P. investigate bacterial adaption antibiotic treatment as well host immune response. Human slices were 10<sup>5</sup> PAO1, treated 10, 20 or 40µg/ml tobramycin incubated for 24h 96h. Tissue viability assessed by...
Chronic <i>P. aeruginosa</i> infections of the respiratory tract pose a major health care problem. The bacterial flagellum represents tightly regulated virulence factor involved in biofilm formation as well immune recognition aeruginosa</i>. Aim this study was to delineate role flagellin using precision-cut lung slices (PCLS) an organotypic, immunocompetent model human lung. PCLS were prepared from tumour-free tissue and infected with wildtype (wt) or mutant (∆<i>fliC</i>) PA14 strains....
Human rhinovirus (HRV) is a main cause of the common cold in humans, and for asthmatics major risk factor exacerbations. HRV targets airway mucosa with host epithelial cells new virions are released without inducing direct cytopathic effect (CPE). Infection by induces an innate immune response secretion cytokines to mediate anti-viral response. The aim study was establish model HRV1B infection human PCLS including prevention induced responses intervention 3C protease inhibitor Rupintrivir....