A5079681254- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Respiratory Support and Mechanisms
- Neuroscience of respiration and sleep
- Cystic Fibrosis Research Advances
- Congenital Diaphragmatic Hernia Studies
- Advanced Drug Delivery Systems
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Neonatal and fetal brain pathology
- Drug Solubulity and Delivery Systems
- Antibiotic Resistance in Bacteria
- Airway Management and Intubation Techniques
- Trypanosoma species research and implications
- Mass Spectrometry Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Infant Nutrition and Health
- Advancements in Transdermal Drug Delivery
- Asthma and respiratory diseases
- Carbohydrate Chemistry and Synthesis
- Hydrogels: synthesis, properties, applications
- Dysphagia Assessment and Management
KUSUDAMA Therapeutics (Spain)
2020-2025
Helmholtz Institute for Pharmaceutical Research Saarland
2015-2023
Helmholtz Centre for Infection Research
2015-2023
Saarland University
2015-2023
GAIKER Technology Centre
2021-2022
KIST Europe
2018
Korea Institute of Science and Technology
2018
BioCruces Health research Institute
2013-2017
Hospital de Cruces
2010-2014
Basque Government
2011
We investigated the rheological properties and penetration of differently sized carboxylated nanoparticles in pig pulmonary mucus, on different distance time scales. Nanoparticles were either mechanically mixed into mucus samples or deposited as an aerosol, latter resembling a more physiologically relevant delivery scenario. After mechanical dispersion, 500 nm particles locally trapped; fraction tracer 100 200 diameter could however freely diffuse these networks over distances approximately...
Abstract Pseudomonas aeruginosa (PA) infections can be notoriously difficult to treat and are often accompanied by the development of antimicrobial resistance (AMR). Quorum sensing inhibitors (QSI) acting on PqsR (MvfR) – a crucial transcriptional regulator serving major functions in PA virulence enhance antibiotic efficacy eventually prevent AMR. An integrated drug discovery campaign including design, medicinal chemistry‐driven hit‐to‐lead optimization in‐depth biological profiling new QSI...
Mucus is a complex hydrogel that acts as protective barrier in various parts of the human body. Both composition and structural properties play crucial role maintaining while dictating diffusion molecules (nano)materials. In this study, we compare previously described mucus surrogates with native airway pig intestinal mucus. Oscillatory shear rheology was applied to characterize on bulk macrorheological level, revealing artificial surrogate deviates from elastic-dominant behavior samples. We...
In the context of cystic fibrosis, Pseudomonas aeruginosa biofilms often develop in vicinity airway mucus, which acts as a protective physical barrier to inhaled matter. However, mucus can also adsorb small drug molecules administered aerosols, including antibiotics, thereby reducing their bioavailability. The efficacy antibiotics is typically assessed by determining MIC using vitro assays. This widespread technique, however, does not consider either bacterial biofilm formation or influence...
Abstract Elimination of pulmonary Pseudomonas aeruginosa (PA) infections is challenging to accomplish with antibiotic therapies, mainly due resistance mechanisms. Quorum sensing inhibitors (QSIs) interfering biofilm formation can thus complement antibiotics. For simultaneous and improved delivery both active agents the infection sites, self‐assembling nanoparticles a newly synthesized squalenyl hydrogen sulfate (SqNPs) were prepared. These nanocarriers allowed for remarkably high loading...
Bronchopulmonary dysplasia (BPD) is a chronic lung condition of premature neonates, yet without an established pharmacological treatment. The BPD rabbit model exposed to 95% oxygen has been used in recent years for drug testing. However, the toxicity strong hyperoxic hit precludes longer-term follow-up due high mortality after first week life. This study aimed extend preterm postnatal day (PND) 14 mimic evolving phase and enable investigation therapeutic interventions at later more relevant...
Bronchopulmonary dysplasia (BPD) is a multifactorial chronic lung disease of premature neonates. BPD development depends on prenatal and postnatal factors that induce inflammation, altering alveolar growth pulmonary vascular development. Animal models are essential to investigate the precise molecular pathways leading BPD. The preterm rabbit combines many advantages small (e.g., rodents) large lambs baboons). Preterm rabbits display mild-to-moderate respiratory distress at delivery, which,...
Abstract The major pathogen found in the lungs of adult cystic fibrosis (CF) patients is Pseudomonas aeruginosa , which builds antibiotic-resistant biofilms. Pulmonary delivery antibiotics by inhalation has already been proved advantageous clinic, but development novel anti-infective aerosol medicines complex and could benefit from adequate vitro test systems. This work describes first model human bronchial epithelial cells cultivated at air–liquid interface (ALI) infected with P. biofilm...
Premature birth, perinatal inflammation, and life-saving therapies such as postnatal oxygen mechanical ventilation are strongly associated with the development of bronchopulmonary dysplasia (BPD); these risk factors, alone or combined, cause lung inflammation alter programmed molecular patterns normal development. The current knowledge on regulation mainly derives from mechanistic studies conducted in newborn rodents exposed to hyperoxia, which have been proven useful but some...
The cell envelope of Gram-negative bacteria is a formidable biological barrier, inhibiting the action antibiotics by impeding their permeation into intracellular environment. In-depth understanding through this barrier remains challenge, despite its critical role in antibiotic activity. We therefore designed divisible vitro model bacterial envelope, mimicking three essential structural elements, inner membrane and periplasmic space as well outer membrane, on Transwell setup. was...
Abstract Lung infections caused by Pseudomonas aeruginosa pose a serious threat to patients suffering from, among others, cystic fibrosis, chronic obstructive pulmonary disease, or bronchiectasis, often leading life-threatening complications. The establishment of infection is substantially related communication between bacteria via quorum-sensing networks. In this study, we aimed assess the role signaling molecules quinolone signal (PQS) and investigate viscoelastic properties lung tissue...
Abstract BACKGROUND Though natural surfactants (SF) are clinically superior to protein‐free synthetic preparations, CHF‐5633, a SF containing SP‐B and SP‐C analog peptides is potential alternative for treating neonatal respiratory distress syndrome (RDS). Nevertheless, information lacking regarding the safety of this new treatment brain. We sought compare cerebral pulmonary effects surfactant (CHF5633) with those porcine (Cursosurf) in premature lambs RDS. METHODS Twenty‐one preterm were...
The delivery of nebulized medications to preterm infants during Non-Invasive Ventilation (NIV) remains an unmet clinical need. In this regard, the effective surfactant has been particularly investigated in preclinical and studies. work, we feasibility delivering through various commercially available nasal prong types. We first performed a compendial characterization aerosols generated by eFlow Neos nebulizer, customized be used neonates, determining amount delivered device as well...