A5085863835- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- HIV-related health complications and treatments
- Food Allergy and Anaphylaxis Research
- Alzheimer's disease research and treatments
- Animal Virus Infections Studies
- Allergic Rhinitis and Sensitization
- Influenza Virus Research Studies
- Digital Imaging for Blood Diseases
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Fern and Epiphyte Biology
- HIV/AIDS Research and Interventions
- vaccines and immunoinformatics approaches
- Vaccine Coverage and Hesitancy
- Respiratory viral infections research
- Infectious Encephalopathies and Encephalitis
- Immune cells in cancer
- Sepsis Diagnosis and Treatment
- Plant and animal studies
Johns Hopkins University
2018-2024
Johns Hopkins Medicine
2018-2024
University of North Carolina at Chapel Hill
2021-2024
Research Institute of Dallas
2023
Illinois College
2015
The key to battling the COVID-19 pandemic and its potential aftermath is develop a variety of vaccines that are efficacious safe, elicit lasting immunity, cover range SARS-CoV-2 variants. Recombinant viral receptor-binding domains (RBDs) safe vaccine candidates but often have limited efficacy due lack virus-like immunogen display pattern. Here we developed novel nanoparticle (VLP) displays 120 copies RBD on surface. This VLP-RBD mimics virus-based in display, which boosts efficacy, while...
Abstract Allergic reactions occur when IgE molecules become crosslinked by antigens such as food proteins. Here we create the ‘AllerScan’ programmable phage display system to characterize binding specificities of anti-allergen IgG and antibodies in serum against thousands allergenic proteins from hundreds organisms at peptide resolution. Using AllerScan, identify robust anti-wheat reactivities wheat allergic individuals but not wheat-sensitized individuals. Meanwhile, a key epitope alpha...
Abstract Whole virus-based inactivated SARS-CoV-2 vaccines adjuvanted with aluminum hydroxide have been critical to the COVID-19 pandemic response. Although these are protective against homologous coronavirus infection, emergence of novel variants and presence large zoonotic reservoirs harboring heterologous coronaviruses provide significant opportunities for vaccine breakthrough, which raises risk adverse outcomes like vaccine-associated enhanced respiratory disease. Here, we use a female...
The alphavirus chikungunya virus (CHIKV) represents a reemerging public health threat as mosquito vectors spread and viruses acquire advantageous mutations. Although primarily arthritogenic in nature, CHIKV can produce neurological disease with long-lasting sequelae that are difficult to study humans. We therefore evaluated immunocompetent mouse strains/stocks for their susceptibility intracranial infection three different strains, the East/Central/South African (ECSA) lineage strain SL15649...
Chronic microglial activation and associated neuroinflammation are key factors in neurodegenerative diseases including HIV-associated neurocognitive disorders. Colony stimulating factor 1 receptor (CSF1R)-mediated signaling is constitutive cells of the myeloid lineage, microglia, promoting cell survival, proliferation, differentiation. In amyotrophic lateral sclerosis Alzheimers disease, CSF1R upregulated. Inhibiting animal models these improved disease outcomes. our studies, CNS expression...
Abstract Our limited understanding of the pathophysiological mechanisms that operate during sepsis is an obstacle to rational treatment and clinical trial design. There a critical lack data from low- middle-income countries where burden increased which inhibits generalized strategies for therapeutic intervention. Here we perform RNA sequencing whole blood investigate longitudinal host response in Ghanaian cohort. Data dimensional reduction reveals dynamic gene expression patterns describe...
Human immunodeficiency virus-associated sensory neuropathy (HIV-SN) remains a frequent neurologic complication of HIV infection. Little is known about alterations in the peripheral nervous system during early stages HIV, time when neuroprotective interventions may be most beneficial. We performed Nanostring gene expression analysis on lumbar dorsal root ganglia (DRG) from 6 simian virus (SIV)-infected pigtailed macaques killed at 7 days post-inoculation and 8 uninfected controls. found...
Abstract HIV-associated neurocognitive disorders (HAND) remain prevalent despite implementation of antiretroviral therapy (ART). Development HAND is linked to mitochondrial dysfunction and oxidative stress in the brain; therefore, upregulation antioxidant defenses critical curtail neuronal damage. Superoxide dismutase 2 (SOD2) a enzyme essential for maintaining cellular viability. We hypothesized that SOD2 was upregulated during retroviral infection. Using simian immunodeficiency virus...
Abstract HIV-associated neurocognitive disorders (HAND) remain prevalent despite implementation of antiretroviral therapy (ART). Development HAND is linked to mitochondrial dysfunction and oxidative stress in the brain; therefore, upregulation antioxidant defenses critical curtail neuronal damage. Superoxide dismutase 2 (SOD2) a enzyme essential for maintaining cellular viability. We hypothesized that SOD2 was upregulated during retroviral infection. Using simian immunodeficiency virus...
Abstract Allergic reactions occur when IgE molecules become crosslinked by antigens such food proteins. The physiologic consequences range from mild to life-threatening. Here we create the ‘AllerScan’ bacteriophage display library for efficiently profiling allergic antibodies characterize binding specificities associated with known allergens. AllerScan enables sensitive and unbiased characterization of circulating IgG antibody reactivities against thousands allergenic proteins hundreds...
Abstract Variable translatability represents a major criticism of the use mouse models for preclinical research. Lack exposure to most endogenous murine pathogens results in natural immune responses specific pathogen free (SPF) lab mice more closely resemble that newborns rather than adult humans. Recently, effects prior infection (PIE) on maturation and have been increasingly explored mice. Here, we employed PIE model where BALB/c underwent sequential subclinical with four at two-week...
<title>Abstract</title> Inactivated whole virus SARS-CoV-2 vaccines adjuvanted with aluminum hydroxide (Alum) are among the most widely used COVID-19 globally and have been critical to pandemic response. Although these protective against homologous infection in healthy recipients, emergence of novel variants presence large zoonotic reservoirs provide significant opportunities for vaccine breakthrough, which raises risk adverse outcomes including vaccine-associated enhanced respiratory...
Abstract Chikungunya virus (CHIKV) is an Old World alphavirus that typically induces arthralgia and rash. However, CHIKV also capable of infecting the central nervous system (CNS), resulting in encephalitis, myelitis, peripheral neuropathy. Patients who survive acute infection have long lasting neurological effects. To date, immunocompetent small animal model recapitulates CNS disease has not been established. develop a mouse infection, we intracranially inoculated 4–6 week old C57BL/6J mice...
Abstract Inactivated vaccines administered with the adjuvant aluminum hydroxide (Alum) are currently most widely used COVID-19 in world and have been critical to responding SARS-CoV-2 pandemic. Although these efficacious against homologous virus infection healthy young adults, emergence of novel variants has resulted significant vaccine breakthrough. Pre-clinical studies inactivated SARS-CoV-1 MERS-CoV reported enhanced immunopathology upon breakthrough infection, characterized by pulmonary...