A5058194660- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Ion channel regulation and function
- Electrochemical Analysis and Applications
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Neurological disorders and treatments
- Neural dynamics and brain function
- Tissue Engineering and Regenerative Medicine
- Analog and Mixed-Signal Circuit Design
- Photoreceptor and optogenetics research
- Electrochemical sensors and biosensors
- Insect and Pesticide Research
- Urological Disorders and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Nicotinic Acetylcholine Receptors Study
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Conducting polymers and applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Genetic Associations and Epidemiology
- Plant and Biological Electrophysiology Studies
Natural and Medical Sciences Institute
2016-2025
University of Tübingen
2015-2024
Reutlingen University
2011-2019
Hahn-Schickard-Gesellschaft für angewandte Forschung
2018
Institut für Mikroelektronik Stuttgart
2018
Saarland University
2007
Yale University
2005
University of Freiburg
1997-2002
To assess the utility of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as an in vitro proarrhythmia model, we evaluated concentration dependence and sources variability electrophysiologic responses to 28 drugs linked low, intermediate, high torsades de pointes (TdP) risk categories using two commercial cell lines standardized protocols a blinded multisite study multielectrode array or voltage-sensing optical approaches. Logistical ordinal linear regression models...
We have examined factors that determine the strength and dynamics of GABAergic synapses between interneurons [dentate gyrus basket cells (BCs)] principal neurons granule (GCs)] using paired recordings in rat hippocampal slices at 34 degrees C. Unitary IPSCs recorded from BC-GC pairs high intracellular Cl(-) concentration showed a fast rise biexponential decay, with mean time constants 2 9 msec. The quantal conductance change, determined directly reduced extracellular Ca(2+)/Mg(2+) ratios,...
AMPA- and NMDA-type glutamate receptors (AMPARs NMDARs) mediate excitatory synaptic transmission in the basal ganglia may contribute to excitotoxic injury. We investigated functional properties of AMPARs NMDARs expressed by six main types neurons acute rat brain slices (principal cholinergic interneurons striatum, GABAergic dopaminergic substantia nigra, globus pallidus neurons, subthalamic nucleus neurons) using fast application nucleated outside-out membrane patches. different were...
Composites of carbon nanotubes and poly(3,4-ethylenedioxythiophene, PEDOT) layers PEDOT are deposited onto microelectrodes by electropolymerization ethylenedioxythiophene in the presence a suspension polystyrene sulfonate. Analysis FIB SEM demonstrates that CNT-PEDOT composites exhibit porous morphology whereas more compact. Accordingly, capacitance charge injection capacity composite material exceed those pure layers. In vitro cell culture experiments reveal excellent biocompatibility...
Recent in vitro cardiac safety studies demonstrate the ability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to detect electrophysiologic effects drugs. However, variability contributed by unique approaches, procedures, cell lines, and reagents across laboratories makes comparisons results difficult, leading uncertainty about role hiPSC-CMs defining proarrhythmic risk drug discovery regulatory submissions. A blinded pilot study was conducted evaluate 8...
The increasing prevalence of diabetes, its heterogeneity, and the limited number treatment options drive need for physiologically relevant assay platforms with human genetic background that have potential to improve mechanistic understanding e\xpedite diabetes-related research treatment. In this study, we developed an endocrine pancreas-on-a-chip model based on a tailored microfluidic platform, which enables self-guided trapping single pseudo-islets. Continuous, low-shear perfusion provides...
Correlative microscopy combining various imaging modalities offers powerful insights into obtaining a comprehensive understanding of physical, chemical, and biological phenomena. In this article, we investigate two approaches for image fusion in the context inherently lower-resolution chemical images obtained using secondary ion mass spectrometry (SIMS) with high-resolution ultrastructural electron (EM). We evaluate methods three different case studies selected to broadly represent typical...
Abstract Neonicotinoid pesticides, originally developed to target the insect nervous system, have been reported interact with human receptors and activate rodent neurons. Therefore, we evaluated in how far these compounds may trigger signaling neurons, thus, affect adult or developing system. We used SH-SY5Y neuroblastoma cells as established model of nicotinic acetylcholine receptor (nAChR) signaling. In parallel, profiled dopaminergic generated from LUHMES neuronal precursor cells, novel...
Several neonicotinoids have recently been shown to activate the nicotinic acetylcholine receptor (nAChR) on human neurons. Moreover, imidacloprid (IMI) and other members of this pesticide family form a set diverse metabolites within crops. Among these, desnitro-imidacloprid (DN-IMI) is special toxicological interest, as there evidence (i) for dietary exposure metabolite, (ii) that DN-IMI strong trigger mammalian responses. We out here quantify responses nAChRs an alternative IMI-olefin. To...
Abstract Human induced pluripotent stem cells (hiPSC) provide an attractive tool to study disease mechanisms of neurodevelopmental disorders such as schizophrenia. A pertinent problem is the development hiPSC-based assays discriminate schizophrenia (SZ) from autism spectrum disorder (ASD) models. Healthy control individuals well patients with SZ and ASD were examined by a panel diagnostic tests. Subsequently, skin biopsies taken for generation, differentiation, testing hiPSC-derived neurons...
To examine possible interactions between fast depression and modulation of inhibitory synaptic transmission in the hippocampus, we recorded from pairs synaptically connected basket cells (BCs) granule (GCs) dentate gyrus rat brain slices at 34 °C. Multiple‐pulse (MPD) was examined trains 5 or 10 postsynaptic currents (IPSCs) evoked frequencies 10–00 Hz under several conditions that inhibit transmitter release: block voltage‐dependent Ca 2+ channels by Cd (10 μ m ), activation γ‐amino‐butyric...
Cardiovascular disease remains a leading cause of mortality and morbidity worldwide. Embryonic stem cell-derived cardiomyocytes (ESC-CMs) may offer significant advances in creating vitro cardiac tissues for modeling, drug testing, elucidating developmental processes; however, the induction ESCs to more adult-like CM phenotype challenging. In this study, we developed bioreactor system employ pulsatile flow (1.48 mL/min), cyclic strain (5%), extended culture time improve maturation murine...
Cell death via necrosis and apoptosis is a hallmark of deep dermal to full-thickness cutaneous burn injuries. Keratinocytes might act as thermosensory cells that transmit information regarding ambient temperature heat-gated transient receptor potential vanilloid (TRPV) ion channels. The aim this study was investigate the distribution TRPV1, 2, 3, 4 in uninjured thermally burned skin. authors investigated warmth-evoked currents keratinocytes cell kinetics injured culture with agonists...
The use of mesenchymal stromal cells (MSCs) differentiated toward a smooth muscle cell (SMC) phenotype may provide an alternative for investigators interested in regenerating urinary tract organs such as the bladder where autologous cannot be used or are unavailable. In this study we measured effects good manufacturing practice (GMP)-compliant expansion followed by myogenic differentiation human MSCs on expression range contractile (from early to late) markers relation electrophysiological...
The leading cause of stress urinary incontinence in women is the urethral sphincter muscle deficiency caused by mechanical during pregnancy and vaginal delivery. In men, prostate cancer surgery injury local nerves muscles are associated with incontinence. Current treatment often fails to satisfy patient's needs. Cell therapy may improve situation. We therefore investigated regeneration potential cells ameliorating a large animal model. Urethral was induced surgically gilts electrocautery...
Neural stem or progenitor cells have been proposed to restore gastrointestinal function in patients suffering from congenital acquired defects of the enteric nervous system. Various, mainly embryonic cell sources identified for this purpose. However, immunological and ethical issues make a postnatal based therapy desirable. We therefore evaluated quantified potential murine system give rise neurons glial vitro. Electrophysiological analysis BrdU uptake studies provided direct evidence that...
Prediction of drug toxicity on the human nervous system still relies mainly animal experiments. Here, we developed an alternative allowing assessment complex signaling in both individual neurons and network level. The LUHMES cultures used for our approach can be cultured 384-well plates with high reproducibility. We established here high-throughput quantification free intracellular Ca2+ concentrations [Ca2+]i as broadly applicable surrogate neuronal activity verified main processes by patch...