A5067986494- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Silk-based biomaterials and applications
- Connective tissue disorders research
- Nerve injury and regeneration
- Proteoglycans and glycosaminoglycans research
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Muscle Physiology and Disorders
- Cardiac Structural Anomalies and Repair
- Cardiac Valve Diseases and Treatments
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- Bone Tissue Engineering Materials
- Tracheal and airway disorders
- RNA Interference and Gene Delivery
- Angiogenesis and VEGF in Cancer
- Medical and Biological Ozone Research
- Photoacoustic and Ultrasonic Imaging
- Laser Applications in Dentistry and Medicine
- S100 Proteins and Annexins
- Tribology and Lubrication Engineering
- Graphene and Nanomaterials Applications
University of Tübingen
2011-2021
Natural and Medical Sciences Institute
2019-2021
Reutlingen University
2021
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2011-2019
Fraunhofer Society
2015
University Children's Hospital Tübingen
2013
University of Stuttgart
2012-2013
Cell and Tissue Systems (United States)
2012
TH Bingen University of Applied Sciences
2012
Currently available heart valve replacements are limited in long-term performance or fail due to leaflet thickening, lack of growth remodeling potential. In order address these issues, it is necessary mimic multiple factors the native valvular extracellular matrix (ECM) such as architecture, mechanical behavior and biochemical signals. Here, we successfully generated an electrospun PEGdma–PLA scaffold adapted structure properties leaflets. Valvular interstitial cells (VICs) endothelial...
Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality implants. We used a newly developed biostable polyurethane elastomer (TPCU) engineer electrospun vascular scaffolds with promising (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 78 mmHg), which were biofunctionalized fibronectin (FN) decorin (DCN). Neither uncoated nor TPCU induced major adverse immune responses except for minor signs polymorph nuclear cell activation....
Chemokine-induced stem cell recruitment is a promising strategy for post myocardial infarction treatment. Injection of stromal cell-derived factor 1 (SDF1) has been shown to attract bone marrow-derived progenitor cells (BMPCs) from the blood that have potential differentiate into cardiovascular cells, which support angiogenesis, enabling improvement function. SDF1-GPVI bi-specific protein contains glycoprotein VI (GPVI)-domain serves as an anchor collagen type I (Col I) and III, are exposed...
Elastic fibers are essential for the proper function of organs including cardiovascular tissues such as heart valves and blood vessels. Although (tropo)elastin production in a tissue-engineered construct has previously been described, assembly to functional elastic vitro using human cells highly challenging. In present study, we seeded primary isolated vascular smooth muscle (VSMCs) onto 3D electrospun scaffolds exposed them defined laminar shear stress customized bioreactor system....
Cardiovascular disease remains a leading cause of mortality and morbidity worldwide. Embryonic stem cell-derived cardiomyocytes (ESC-CMs) may offer significant advances in creating vitro cardiac tissues for modeling, drug testing, elucidating developmental processes; however, the induction ESCs to more adult-like CM phenotype challenging. In this study, we developed bioreactor system employ pulsatile flow (1.48 mL/min), cyclic strain (5%), extended culture time improve maturation murine...
Actinic Keratosis' (AKs) are small skin lesions that related to a prolonged sun-damage, which can develop into invasive squamous cell carcinoma (SCC) when left untreated. Effective, specific and well tolerable therapies cure AKs still of great interest. Diclofenac (DCF) is the current gold standard for local treatment in terms costs, effectiveness, side effects tolerability. In this work, an electrospun polylactic acid (PLA) scaffold loaded with synthetic DCF prodrug was developed...
Decorin (DCN) is an important small leucine-rich proteoglycan present in the extracellular matrix (ECM) of many organs and tissues. Endothelial progenitor cells (EPCs) are able to interact with surrounding ECM bind molecules such as DCN. Here, we recombinantly produced full-length human DCN under good laboratory practice (GLP) conditions, after detailed immunological characterization, investigated its potential attract murine EPCs (mEPCs hECFCs). Electrospun polymeric scaffolds were coated...
Electrospinning is an attractive method to generate drug releasing systems. In this work, we encapsulated the cell death-inducing Diclofenac (DCF) in electrospun poly-L-lactide (PLA) scaffold. The scaffold offers a system for sustained and controlled delivery of cytotoxic DCF over time making it clinically favourable by achieving prolonged therapeutic effect. We exposed human dermal fibroblasts (HDFs) drug-eluting employed multiphoton microscopy fluorescence lifetime imaging microscopy....
In this work we generated hybrid gelatin:poly-<sc>l</sc>-lactide electrospun scaffolds and implemented non-invasive methods to characterize them.
Fibronectin coating increases implant biocompatibility by enhancing surface endothelialization via integrin-mediated binding. Surface properties determine the fibronectin orientation and conformation, dictating which ligands are presented, therefore altering bioactivity of an surface. In this study, polyurethane was treated with oxygen plasma, allowed for a simultaneous modification chemistry topography to modulate adsorption. By varying parameters treatment, human plasma adsorbed on...
Osteoarthritis (OA) impacts hundreds of millions people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for development OA. Current cartilage repair strategies moderately effective at reducing pain but often replace damaged tissue biomechanically inferior fibrocartilage. Here we describe development, transcriptomic ontogenetic characterization quality assessment...
Bioreactors are essential cell and tissue culture tools that allow the introduction of biophysical signals into in vitro cultures. One major limitation is need to interrupt experiments sacrifice samples at certain time points for analyses. To address this issue, we designed a bioreactor combines high-resolution contact-free imaging continuous flow closed system compatible with various types microscopes. The high throughput fluid was combined two-photon fluorescence lifetime microscopy...
The immune system is widely recognized as a crucial determinant in implant biocompatibility and tissue integration. In order to assess macrophage response biomaterials, commonly used analysis techniques require the removal of cells from material. This process inherently has negative impact on cells, but few studies have comprehensively compared different dissociation methods terms cell yield, viability, phenotype function. Cell scraping, EDTA at 4 °C, 37 trypsin, Accutase PromoCell...
In this study, we describe the production of hybrid gelatin–poly-L-lactide electrospun scaffolds whose hydrophilicity was controlled by binding increasing concentrations hyaluronic acid (HA). We show that cross-linking has advantages over coating when aiming to functionalize with HA. The here described structurely mimicked complexity extracellular matrix, and excited second harmonic generation, they produced a signal is typical collagen-containing biological fibers. Fluorescence lifetime...