A5003595309- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- Advanced Nanomaterials in Catalysis
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Renal Diseases and Glomerulopathies
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Electrospun Nanofibers in Biomedical Applications
- Polyomavirus and related diseases
- Cardiac Structural Anomalies and Repair
Max Delbrück Center
2020-2024
Charité - Universitätsmedizin Berlin
2020-2024
Trinity College Dublin
2019
Non-viral gene editing via CRISPR-Cas12a offers an alternative to Cas9-based methods, providing better targeting of AT-rich regions, simplified guide RNA manufacturing, and high specificity. However, the efficacy outcomes is subject various factors, with tem-plate format playing a crucial role. Currently, predominant non-viral template for inducing homology-directed repair (HDR) after nuclease-induced DNA breaks double-stranded (dsDNA), which toxic when transfected at doses. Previous studies...