A5039999144- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Caveolin-1 and cellular processes
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- Retinal Development and Disorders
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Boron Compounds in Chemistry
- Hippo pathway signaling and YAP/TAZ
- Advanced biosensing and bioanalysis techniques
- Advanced Electron Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Nerve injury and regeneration
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Hereditary Neurological Disorders
- Pancreatic function and diabetes
- Ubiquitin and proteasome pathways
- Photoreceptor and optogenetics research
Friedrich Schiller University Jena
2016-2025
Jena University Hospital
2016-2025
Karolinska Institutet
2021
Northeast Normal University
2021
Leibniz Institute for Neurobiology
2002-2014
Institute of Human Genetics
2014
Charité - Universitätsmedizin Berlin
2009
University of California, Berkeley
1998-2005
Institute of Groundwater Ecology
1999
Institut für Tier-, Natur- und Umweltethik
1996
The ribbon complex of retinal photoreceptor synapses represents a specialization the cytomatrix at active zone (CAZ) present conventional synapses. In mice deficient for CAZ protein Bassoon, ribbons are not anchored to presynaptic membrane but float freely in cytoplasm. Exploiting this phenotype, we dissected molecular structure complex. Identifiable proteins segregate into two compartments ribbon: ribbon-associated compartment including Piccolo, RIBEYE, CtBP1/BARS, RIM1, and motor KIF3A, an...
Abstract The endoplasmic reticulum (ER) undergoes continuous remodelling via a selective autophagy pathway, known as ER-phagy 1 . receptors have central role in this process 2 , but the regulatory mechanism remains largely unknown. Here we report that ubiquitination of receptor FAM134B within its reticulon homology domain (RHD) promotes clustering and binding to lipidated LC3B, thereby stimulating ER-phagy. Molecular dynamics (MD) simulations showed how perturbs RHD structure model bilayers...
Membrane-shaping proteins characterized by reticulon homology domains play an important part in the dynamic remodelling of endoplasmic reticulum (ER). An example such a protein is FAM134B, which can bind LC3 and mediate degradation ER sheets through selective autophagy (ER-phagy)1. Mutations FAM134B result neurodegenerative disorder humans that mainly affects sensory autonomic neurons2. Here we report ARL6IP1, another ER-shaping contains domain associated with loss3, interacts participates...
Huntingtin-interacting protein 1 related (Hip1R) is a novel component of clathrin-coated pits and vesicles mammalian homologue Sla2p, an actin-binding important for both actin organization endocytosis in yeast. Here, we demonstrate that Hip1R binds via its putative central coiled-coil domain to clathrin, provide evidence clathrin are associated vivo at sites endocytosis. First, real-time analysis Hip1R-YFP DsRed-clathrin light chain (LC) live cells revealed these proteins show almost...
The actin cytoskeleton has been implicated in endocytosis, yet few molecular links to the endocytic machinery have established. Here we show that mammalian F-actin-binding protein Abp1 (SH3P7/HIP-55) can functionally link dynamin, a GTPase functions endocytosis. binds directly dynamin vitro through its SH3 domain. Coimmunoprecipitation and colocalization studies demonstrated vivo relevance of this interaction. In neurons, colocalized at actin-rich sites proximal cell body during...
The actin cytoskeleton has been implicated in endocytosis, yet few molecules that link these systems have identified. Here, we cloned and characterized mHip1R, a protein is closely related to huntingtin interacting 1 (Hip1). These two proteins are mammalian homologues of Sla2p, an binding important for organization endocytosis yeast. Sequence alignments secondary structure predictions verified mHip1R belongs the Sla2 family. Thus, contains NH2-terminal domain homologous Sla2p's endocytic...
Actin has well established functions in cellular morphogenesis. However, it is not understood how the various actin assemblies a cell are kept dynamic equilibrium, particular when cells have to respond acute signals. Here, we characterize rapid and transient reset response increased intracellular calcium levels. Within seconds of influx, formin INF2 stimulates filament polymerization at endoplasmic reticulum (ER), while cortical disassembled. The reaction then reversed within few minutes....
Hereditary spastic paraplegia (HSP) is characterized by a dying back degeneration of corticospinal axons which leads to progressive weakness and spasticity the legs. SPG11 most common autosomal-recessive form HSPs caused mutations in SPG11. A recent vitro study suggested that Spatacsin, respective gene product, needed for recycling lysosomes from autolysosomes, process known as autophagic lysosome reformation. The relevance this observation hereditary paraplegia, however, has remained...
In response to different types and intensities of mechanical force, cells modulate their physical properties adapt plasma membrane (PM). Caveolae are PM nano-invaginations that contribute mechanoadaptation, buffering tension changes. However, whether core caveolar proteins accommodation independently from the assembly is unknown. Here we provide experimental computational evidence supporting caveolin-1 confers deformability mechanoprotection caveolae, through modulation curvature....
EHD proteins were shown to function in the exit of receptors and other membrane from endosomal recycling compartment. Here, we identify syndapins, accessory vesicle formation at plasma membrane, as differential binding partners for proteins. These complexes are formed by direct eps15-homology (EH) domain/asparagine proline phenylalanine (NPF) motif interactions. Heterologous endogenous coimmunoprecipitations well reconstitutions syndapin/EHD protein intracellular membranes living cells...
Yeast Abp1p is a cortical actin cytoskeleton protein implicated in cytoskeletal regulation, endocytosis, and cAMP-signaling. We have identified gene encoding mouse homologue of Abp1p, it identical to SH3P7, shown recently be target Src tyrosine kinases. display the same domain structure including an N-terminal actin-depolymerizing factor homology C-terminal 3 domain. Using two independent actin-binding domains, mAbp1 binds filaments with 1:5 saturation stoichiometry. In stationary cells,...
Synaptic contacts contain elaborate cytomatrices on both sides of the synaptic cleft, which are believed to organize and link different functions in time space can respond inner outer cues with massive structural reorganizations. At PSD (postsynaptic density), activity-dependent reorganizations cortical actin cytoskeleton hypothesized play a role plasticity. Here, we report interactions F-actin binding protein Abp1 members ProSAP/Shank family: multidomain scaffolding proteins interconnecting...
Coordinated functions of the actin cytoskeleton and microtubules, which require careful control in time space, are indispensable for drastic alterations neuronal morphology during neuromorphogenesis network formation. Actin filament formation driven by Arp2/3 complex its activator neural Wiskott-Aldrich syndrome protein (N-WASP) is important proper axon development. The underlying molecular mechanisms targeting to specific activation N-WASP at plasma membrane, however, have thus far remained...
Hereditary spastic paraplegias (HSPs) are characterized by progressive weakness and spasticity of the legs because degeneration cortical motoneuron axons. SPG15 is a recessively inherited HSP variant caused mutations in ZFYVE26 gene additionally cerebellar ataxia, mental decline, thinning corpus callosum. encodes FYVE domain-containing protein ZFYVE26/SPASTIZIN, which has been suggested to be associated with newly discovered adaptor 5 (AP5) complex. We show that Zfyve26 broadly expressed...