A5065332810- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Thermography and Photoacoustic Techniques
- Optical Coherence Tomography Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Air Quality and Health Impacts
- Advanced X-ray and CT Imaging
- Air Quality Monitoring and Forecasting
- Cutaneous Melanoma Detection and Management
- Medical and Biological Ozone Research
- Mobile Ad Hoc Networks
- Advanced Biosensing Techniques and Applications
- Ultrasound and Hyperthermia Applications
- Inflammatory Bowel Disease
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- Infrared Thermography in Medicine
- Virus-based gene therapy research
- Opportunistic and Delay-Tolerant Networks
- Spectroscopy and Chemometric Analyses
- Vehicular Ad Hoc Networks (VANETs)
iThera Medical (Germany)
2013-2022
DKFZ-ZMBH Alliance
2019
German Cancer Research Center
2019
Technical University of Munich
2011-2015
Helmholtz Zentrum München
2011-2015
Optoacoustic imaging strategies can be used to identify metastasis in excised lymph nodes and determine SLN status patients noninvasively.
Detection of intrinsic or extrinsically administered chromophores and photo-absorbing nanoparticles has been achieved by multi-spectral optoacoustic tomography (MSOT). The detection sensitivity MSOT depends not only on the signal to noise ratio considerations, as in conventional (photoacoustic) implementations, but also ability resolve molecular targets interest from absorbing tissue background means spectral unmixing sub-pixel methods. However, it is known which methods are optimally suited...
The characterization of pharmacokinetic and biodistribution profiles is an essential step in the development process new candidate drugs or imaging agents. Simultaneously, assessment organ function related to uptake clearance great importance. To this end, we demonstrate platform capable high-rate dynamics fluorescent agents multiple organs using multispectral optoacoustic tomography (MSOT). A spatial resolution approximately 150 µm through mouse cross-sections allowed us image blood...
We reported earlier the delivery of antiangiogenic single chain antibodies by using oncolytic vaccinia virus strains to enhance their therapeutic efficacy. Here, we provide evidence that gene-evoked production melanin can be used as a and diagnostic mediator, exemplified insertion only one or two genes into genome an strain. found produced is excellent reporter for optical imaging without addition substrate. Melanin also facilitated deep tissue optoacoustic well MRI. In addition, was shown...
Optoacoustic tomography is a fast developing imaging modality, combining the high contrast available from optical excitation of tissue with resolution and penetration depth ultrasound detection. Light subject to both absorption scattering when traveling through tissue; adequate knowledge properties hence spatial fluence distribution required create an optoacoustic image that directly proportional chromophore concentrations at all depths. Using data commercial multispectral (MSOT) system, we...
Implementation of hybrid imaging using optoacoustic tomography (OAT) and ultrasound (US) brings together the important advantages complementary features both methods. However, fundamentally different physical contrast mechanisms two modalities may impose significant difficulties in optimal tomographic data acquisition image formation strategies. We investigate applicability commonly applied geometries for reconstruction pulse-echo (OPUS) images. Optimization strategy concave array geometry...
A common side effect of medication is gastrointestinal intolerance. Symptoms can include reduced appetite, diarrhea, constipation, GI inflammation, nausea and vomiting. Such effects often have a dramatic impact on compliance with treatment regimen. Therefore, characterization tolerance an important step when establishing novel therapeutic approach. In this study, Multispectral Optoacoustic Tomography (MSOT) used to monitor motility by in vivo whole body imaging mice. MSOT combines high...
The Hessian-based Frangi vesselness filter is commonly used to enhance vasculature in optoacoustic (photoacoustic) images, but its accuracy and limitations have never been rigorously assessed. Here we validate the ability of vessel-like structures phantoms, introduce an experimental approach that uses measurements before after administration gold nanorods (AuNRs) examine performance vivo. We evaluate influence contrast, scales, angular tomographic coverage, out-of-plane signals light fluence...
The highly complementary information provided by multispectral optoacoustics and pulse-echo ultrasound have recently prompted development of hybrid imaging instruments bringing together the unique contrast advantages both modalities. In optoacoustic (OPUS) combination, images retrieved one modality may further be used to improve reconstruction accuracy other. this regard, image segmentation plays a major role as it can aid improving quality quantification abilities facilitating modeling...
MultiSpectral Optoacoustic Tomography (MSOT) is an emerging imaging technology that allows for data acquisition at high spatial and temporal resolution. These characteristics are advantageous Dynamic Contrast Enhanced (DCE) can assess the combination of vascular flow permeability. However, quantitative analysis DCE MSOT has not been possible due to complications caused by wavelength-dependent light attenuation variability in fluence different anatomical locations. In this work we present a...
Photoacoustic imaging (PAI) is an emerging modality that has shown promise for improving patient management in a range of applications. Unfortunately, the current lack uniformity PAI data formats compromises inter-user exchange and comparison, which impedes: technological progress; effective research collaboration; efforts to deliver multi-centre clinical trials. To overcome this challenge, International Standardisation Consortium (IPASC) established format with defined consensus metadata...
We introduce a new approach to detect individual microparticles that contain NIR fluorescent dye by multispectral optoacoustic tomography in the context of hemoglobin-rich environment within murine liver. encapsulated near infrared (NIR) polystyrene microspheres, then injected them into ileocolic vein, which drains absorption was determined using tomography. To quantitate minimum diameter we used both colorimetric and spatial information segment regions microspheres appear. Regional...
Abstract Purpose Optoacoustic imaging with ultrasound (OPUS) can assess in-vivo perfusion/oxygenation through surrogate measures of oxy, deoxy and total hemoglobin content in tissues. The primary aim our study was to evaluate the ability OPUS detect physiological changes breast during menstrual cycle determine qualitative/quantitative metrics normal parenchymal tissue pre-/post-menopausal women. secondary technique’s repeatability. Materials Methods We performed a prospective ethically...
Photoacoustic Imaging (PAI) is a rapidly emerging imaging modality that based on the conversion from light into ultrasound. One of most promising applications PAI in diagnosis and longitudinal monitoring solid tumours. By visualizing differences optical energy absorbed by endogenous chromophores, such as haemoglobin or melanin, has made rapid advances over past decade. In particular, been successfully demonstrated early stage human trials cancer, for delineating benign malignant lesions well...
Multispectral optoacoustic tomography (MSOT) offers the unique capability to map distribution of spectrally distinctive endogenous and exogenous substances in heterogeneous biological tissues by exciting sample at various wavelengths detecting optoacoustically-induced ultrasound waves. This powerful functional molecular imaging can greatly benefit from hybridization with pulse-echo (US), which provides additional information on tissue anatomy blood flow. However, speed sound variations...
Multispectral optoacoustic (photoacoustic) tomography (MSOT) exploits high resolutions given by ultrasound detection technology combined with deeply penetrating laser illumination in the near infrared. Traces of molecules different spectral absorption profiles, such as blood (oxy- and de-oxygenated) biomarkers can be recovered using multiple wavelengths excitation a set methods described this work. Three unmixing are examined for their performance decomposing images into components order to...
The International Photoacoustic Standardisation Consortium (IPASC) emerged from SPIE 2018, established to drive consensus on photoacoustic system testing. As imaging (PAI) matures research laboratories into clinical trials, it is essential establish best-practice guidelines for image acquisition, analysis and reporting, a standardised approach technical validation. primary goal of the IPASC create widely accepted phantoms testing preclinical PAI systems. To achieve this, has formed five...
Multispectral optoacoustic (photoacoustic) tomography (MSOT) exploits the high resolutions provided by ultrasound imaging technology in combination with more biologically relevant optical absorption contrast. Traces of molecules different spectral profiles, such as blood (oxy- and de-oxygenated) biomarkers can be recovered using multiple wavelengths excitation a set methods described this work. Three unmixing are examined for their performance decomposing images into components order to...