A5050707905- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Polymer Surface Interaction Studies
- Vibrio bacteria research studies
- Cell Image Analysis Techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Micro and Nano Robotics
- Pickering emulsions and particle stabilization
- Photoacoustic and Ultrasonic Imaging
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Genomics and Phylogenetic Studies
- Dendrimers and Hyperbranched Polymers
- 3D Printing in Biomedical Research
- Blood properties and coagulation
- Electrohydrodynamics and Fluid Dynamics
- Advanced Fluorescence Microscopy Techniques
- Fungal and yeast genetics research
Philipps University of Marburg
2013-2022
Max Planck Institute for Terrestrial Microbiology
2016-2021
Loewe Center for Synthetic Microbiology
2020
Here we have investigated the effect of enshrouding polymer-coated nanoparticles (NPs) with polyethylene glycol (PEG) on adsorption proteins and uptake by cultured cells. PEG was covalently linked to polymer surface maximal grafting density achievable under our experimental conditions. Changes in effective hydrodynamic radius NPs upon human serum albumin (HSA) fibrinogen (FIB) were measured situ using fluorescence correlation spectroscopy. For without a shell, thickness increase around 3 nm,...
Upon incorporation of nanoparticles (NPs) into the body, they are exposed to biological fluids, and their interaction with dissolved biomolecules leads formation so-called protein corona on surface NPs. The composition plays a crucial role in fate While effects various physicochemical parameters have been explored depth, temperature upon its has received much less attention. In this work, we probed effect set NPs chemistries electric charges. Our results indicate that degree coverage...
Abstract Biofilms are microbial communities that represent a highly abundant form of life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space time; microscopy quantitative image analysis therefore crucial for elucidating their functions. Here, we present BiofilmQ—a comprehensive cytometry software tool the automated high-throughput quantification, visualization numerous biofilm-internal whole-biofilm properties time.
Polyelectrolyte multilayer (PEM) capsules are carrier vehicles with great potential for biomedical applications. With the future aim of designing biocompatible, effective therapeutic delivery systems (e.g., cancer), pathway internalization (uptake and fate) PEM was investigated. In particular following experiments were performed: (i) study capsule co-localization established endocytic markers, (ii) switching-off endocytotic pathways pharmaceutical/chemical inhibitors, (iii) characterization...
Abstract The physico‐chemical properties of colloidal particles determine their uptake into cells. For a series microparticles only one parameter, the mechanical stiffness, was varied, whereas other parameters such as size, shape, and charge were kept constant. monitored in situ by analyzing individual particle trajectories including progress endocytosis, derived from local pH measurements around each particle. Evidence is presented that soft with low stiffness are transported faster to...
Abstract A homologous nanoparticle library was synthesized in which gold nanoparticles were coated with polyethylene glycol, whereby the diameter of cores, as well thickness shell varied. Basic physicochemical parameters this two‐dimensional library, such size, ζ‐potential, hydrophilicity, elasticity, and catalytic activity ,were determined. Cell uptake selected equal size yet varying polymer their effect on basic structural functional cell Data indicates that thinner, more hydrophilic...
Abstract Time-resolved quantitative colocalization analysis is a method based on confocal fluorescence microscopy allowing for sophisticated characterization of nanomaterials with respect to their intracellular trafficking. This technique was applied relate the internalization patterns nanoparticles i . e superparamagnetic iron oxide distinct physicochemical characteristics uptake mechanism, rate and fate. The showed particles approximately same size shape as well similar magnetic...
Smart nanoenvironments were obtained by cell-imprinted substrates based on mature and dedifferentiated chondrocytes as templates. Rabbit adipose derived mesenchymal stem cells (ADSCs) seeded these driven to adopt the specific shape (as determined in terms of cell morphology) molecular characteristics gene expression) types which had been used template for cell-imprinting. This method might pave way a reliable, efficient, cheap controlling differentiation. Data also suggest that besides...
Significance Most living systems, from individual cells to tissues and swarms, display collective self-organization on length scales that are much larger than those of the units drive this organization. A fundamental challenge is understand how properties microscopic components determine macroscopic, multicellular biological function. Our study connects intracellular physiology macroscale behaviors during development, spanning five orders magnitude in six time, using bacterial swarming as a...
Photothermal release of cargo molecules has been extensively studied for bioapplications. For instance, microcapsules decorated with plasmonic nanoparticles have widely used in vitro assays. However, some concerns about their suitability vivo applications cannot be easily overcome, particular the limited penetration depth light (even infrared). Magnetic are alternative heat-mediators local heating, which can triggered by applying an alternating magnetic field (AMF). AMFs much less absorbed...
The synthesis of stimuli-responsive DNA microcapsules acting as carriers for different payloads, and being dissociated through the formation aptamer–ligand complexes is described. Specifically, anti-adenosine triphosphate (ATP) aptamer-cross-linked DNA-stabilized loaded with tetramethylrhodamine-modified dextran (TMR-D), CdSe/ZnS quantum dots (QDs), or microperoxidase-11 (MP-11) are presented. In presence ATP trigger, aptamer–ATP complexes, resulting in release respective loads. Selective...
Bacteria can generate benefits for themselves and their kin by living in multicellular, matrix-enclosed communities, termed biofilms, which are fundamental to microbial ecology the impact bacteria have on environment, infections, industry [1-6]. The advantages of biofilm mode life include increased stress resistance access concentrated nutrient sources [3, 7, 8]. However, there also costs associated with growth, including metabolic burden matrix production, resource competition, limited...
Exposure of cells to colloidal nanoparticles (NPs) can have concentration-dependent harmful effects. Mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e., viability assays, gene expression profiles, etc., neglecting that the presence NPs also drastically affect cellular morphology. In case polymer-coated Au NPs, we demonstrate upon NP internalization, undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances actin and...
Abstract Prokaryotic cells display a striking subcellular organization. Studies of the underlying mechanisms in different species have greatly enhanced our understanding morphological and physiological adaptation bacteria to environmental niches. The image analysis software tool BacStalk is designed extract comprehensive quantitative information from images morphologically complex with stalks, flagella, or other appendages. resulting data can be visualized interactive demographs, kymographs,...
Bacteria commonly live in spatially structured biofilm assemblages, which are encased by an extracellular matrix. Metabolic activity of the cells inside biofilms causes gradients local environmental conditions, leads to emergence physiologically differentiated subpopulations. Information about properties and spatial arrangement such metabolic subpopulations, as well their interaction strength length scales lacking, even for model systems like
We have studied the effect of zwitterionic surface coating quantum dots (QDs) on their interaction with a serum supplemented cell medium and internalization by human cervical carcinoma (HeLa) cells. Zwitterionic QDs showed negligible adsorption albumin (HSA) selected as model protein, in contrast to similar but negatively charged QDs. The incorporation HeLa cells was found be lower than for positively QDs, which uptake yield largest. Our results suggest that suppression protein adsorption,...
remains a major global health threat, disproportionately impacting parts of the world without adequate infrastructure and sanitation resources. In aquatic environments,
Biofilm formation is critical for the infection cycle of Vibrio cholerae. exopolysaccharides (VPS) and matrix proteins RbmA, Bap1 RbmC are required development biofilm architecture. We demonstrate that RbmA binds VPS directly uses a binary structural switch within its first fibronectin type III (FnIII-1) domain to control dynamics VPS-dependent higher-order structures. The in FnIII-1 regulates interactions trans with FnIII-2 domain, leading open (monomeric) or closed (dimeric) interfaces....