A5088562722- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Tryptophan and brain disorders
- Viral-associated cancers and disorders
- Calcium signaling and nucleotide metabolism
- Immune cells in cancer
- Antimicrobial Peptides and Activities
- Erythrocyte Function and Pathophysiology
- Chemokine receptors and signaling
- Cellular transport and secretion
- Cytomegalovirus and herpesvirus research
- Cancer Immunotherapy and Biomarkers
- Nanoparticles: synthesis and applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Rabies epidemiology and control
- Tuberculosis Research and Epidemiology
- Magnesium in Health and Disease
- Mycobacterium research and diagnosis
- Graphene and Nanomaterials Applications
University Hospital of Basel
2022-2024
University of Basel
2016-2024
Harvard University
2021
Massachusetts General Hospital
2021
After infection of B cells, Epstein-Barr virus (EBV) engages host pathways that mediate cell proliferation and transformation, contributing to the propensity drive immune dysregulation lymphomagenesis. We found EBV protein EBNA2 initiates nicotinamide adenine dinucleotide (NAD) de novo biosynthesis by driving expression metabolic enzyme indoleamine 2,3-dioxygenase 1 (IDO1) in infected cells. Virus-enforced NAD production sustained mitochondrial complex I activity, match adenosine...
ABSTRACT Antibiotic development and treatment focus on bacterial growth inhibition, often with limited success. Here, we introduce Antimicrobial Single-Cell Testing (ASCT), an advanced imaging strategy to assess killing in real-time. By tracking 140 million bacteria generating over 20,000 vitro time-kill curves, can predict Mycobacterium tuberculosis outcomes mice humans link strain-specific survival (drug tolerance) abscessus clinical responses. Using ASCT, reveal drug tolerance as a...
Dendritic cells (DCs) actively sample and present antigen to of the adaptive immune system are thus vital for successful control memory formation. Immune cell metabolism function tightly interlinked, a better understanding this interaction offers potential develop immunomodulatory strategies. However, current approaches assessing metabolome often limited by end-point measurements, may involve laborious preparation, lack unbiased, temporal resolution metabolome. In study, we novel setup...
Abstract Amoeboid cell migration is key to efficient T immunity. Spatial polarization of organelles within cells, including endo-lysosomes, a prerequisite migration. However, how ultrastructural linked the signaling requirements governing migration, remains unknown. Here we show that molecules generated by endo-lysosome-localized kinases regulate velocity amoeboid Specifically, imaging cells identified accumulation endo-lysosomes decorated with lipid VPS34–PIKfyve at uropod polarized cells....
The generation of effector CD8+ T cells (TEFF) requires activation naive (TN) by dendritic (DCs) within lymphoid tissue. To date, it remains elusive how the duration TN-DC interactions and integration signals are controlled in vivo. Here, we report that stroma-secreted ligands for CCR7 constrained interaction gradually inducing cell release from DCs. At late time points interactions, repositioned F-actin-promoting factor DOCK2 away DC interface to enable detachment, proliferation onset...
Abstract Naïve T cells and dendritic (DCs) are drawn to lymphoid tissue by the chemokine receptor CCR7, where its ligands CCL19 CCL21 secreted stroma. There, CCR7 fuels fast migration of naïve expedite encounters with DCs presenting cognate pMHC, leading formation long-lasting contacts. Direct in situ observations mouse models have shown that after approximately 12–24 h, activated detach from before undergoing cell division effector differentiation. Yet, mechanisms underlying release DCs,...