A5055443405- Neuroscience and Neuropharmacology Research
- Fungal and yeast genetics research
- Genetics, Aging, and Longevity in Model Organisms
- Ion channel regulation and function
- Pluripotent Stem Cells Research
- Cellular transport and secretion
- Antibiotic Resistance in Bacteria
- Glycosylation and Glycoproteins Research
- Antibiotic Use and Resistance
- Craniofacial Disorders and Treatments
- Lipid Membrane Structure and Behavior
- Biomedical Ethics and Regulation
- Cleft Lip and Palate Research
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Cystic Fibrosis Research Advances
- Epilepsy research and treatment
- Phagocytosis and Immune Regulation
- Anesthesia and Neurotoxicity Research
- Advanced MRI Techniques and Applications
- Congenital heart defects research
- Toxin Mechanisms and Immunotoxins
- Neonatal and fetal brain pathology
- Neonatal Respiratory Health Research
- Nicotinic Acetylcholine Receptors Study
Howard University
2013-2025
Howard University Hospital
2020
California Institute for Regenerative Medicine
2018-2019
Pfizer (China)
2013-2018
Pfizer (United States)
2004-2013
Houston Methodist
2009
Methodist Hospital
2009
Rice University
2009
Cornell University
2009
Howard Hughes Medical Institute
2006
Use of clinical-grade human induced pluripotent stem cell (iPSC) lines as a starting material for the generation cellular therapeutics requires demonstration comparability derived from different individuals and in facilities. This agreement on critical quality attributes such assays that should be used. Working established recommendations guidance International Stem Cell Banking Initiative embryonic banking, concentrating those issues more relevant to iPSCs, series consensus workshops has...
Yeast deprived of nutrients exhibit a marked life span extension that requires the activity NAD<sup>+</sup>-dependent histone deacetylase, Sir2p. Here we show increased dosage <i>NPT1</i>, encoding nicotinate phosphoribosyltransferase critical for NAD<sup>+</sup> salvage pathway, increases Sir2-dependent silencing, stabilizes rDNA locus, and extends yeast replicative by up to 60%. Both <i>NPT1</i> <i>SIR2</i> provide resistance against heat shock, demonstrating these genes act in more...
A relationship between life span and cellular glucose metabolism has been inferred from genetic manipulations caloric restriction of model organisms. In this report, we have used the Snf1p glucose-sensing pathway ofSaccharomyces cerevisiae to explore biochemical linkages aging. is a serine/threonine kinase that regulates responses deprivation. Loss Snf4p, an activator Snf1p, extends generational whereas loss Sip2p, presumed repressor kinase, causes accelerated aging phenotype. An annotated...
For a number of organisms, the ability to withstand periods nutrient deprivation correlates directly with lifespan. However, underlying molecular mechanisms are poorly understood. We show that deletion N-myristoylprotein, Sip2p, reduces resistance and shortens lifespan in Saccharomyces cerevisiae. This reduced is due accelerated aging, as defined by loss silencing from telomeres mating loci, nucleolar fragmentation, accumulation extrachromosomal rDNA. Genetic studies indicate sip2Delta...
The Regional Resistance Surveillance program monitored susceptibility rates and developing resistance by geographic region, including 12 Asia-Pacific (APAC) countries. Reference broth microdilution methods for susceptibility/interpretations were applied, processing 5,053 strains. Among Staphylococcus aureus isolates (37% methicillin-resistant S. [MRSA], highest in South Korea [73%]), linezolid (LZD), tigecycline (TIG), vancomycin 100% active, but 33 34% of strains levofloxacin (LEV) or...
Anti-apoptotic activity of BCL-2 is mediated by phosphorylation at the endoplasmic reticulum (ER), but how this regulated and mechanism(s) which it regulates apoptosis are unknown. We purified macromolecular complexes containing from ER membranes found that co-purified with main two subunits serine/threonine phosphatase, PP2A. The association endogenous PP2A was verified co-immunoprecipitation microcystin affinity purification. Knock down or pharmacological inhibition caused degradation...
Saccharomyces cerevisiae has evolved a number of mechanisms for sensing glucose. In the present study we examine mechanism by which one these pathways, involving Snf1, regulates cellular aging. Snf1 is heterotrimer composed catalytic α subunit (Snf1p) that phosphorylates target proteins at Ser/Thr residues, an activating γ (Snf4p), and β (Sip1p, Sip2p, or Gal83). We previously showed forced expression Snf1p loss but not other subunits, causes accelerated aging, while removal Snf4p extends...
With their unique nanoscalar properties, single-walled carbon nanotube (SWNT) materials are widely studied for various biological applications. Herein, we report the efficiency of full-length HiPco SWNTs and ultra-short (US-tubes) as T2-weighted MRI contrast agents. Analysis has concluded that superparamagnetic SWNT (especially US-tubes) a new class high-efficacy agents having performance contributions from both iron catalyst nanoparticles (originating synthesis materials) material itself....
Zika, a mosquito-borne flavivirus, has been found in 87 countries and territories. Global outbreaks peaked 2016. Prenatal infection of Zika virus was to be associated with microcephaly, arthrogryposis, intracranial calcifications, fetal growth restriction, demise. The most severely affected children were diagnosed congenital syndrome, which impacts thousands worldwide. With no approved treatment or preventative measures for future viral have the potential cause epidemic levels prenatal brain...
It is well known that opiate addiction a neurobiological disease associated with dysregulation of multiple neurotransmitters and neurochemicals. Previous ex-vivo 1H nuclear magnetic resonance (NMR) studies have yielded mixed findings concerning opiate-induced neurometabolic changes at key reward-addiction sites. Whether such reflect the conditions in live animal remains unknown. The present study was therefore designed to fill this knowledge gap by determining effects morphine-induced under...
<title>Abstract</title> Zika, a mosquito-borne flavivirus, has been found in 87 countries and territories. Global outbreaks peaked 2016. Prenatal infection of Zika virus was to be associated with microcephaly, arthrogryposis, intracranial calcifications, fetal growth restriction, demise. The most severely affected children were diagnosed congenital syndrome, which impacts thousands worldwide. With no approved treatment or preventative measures for future viral have the potential cause...
In Duchenne muscular dystrophy (DMD), a genetic disruption of dystrophin protein expression results in repeated muscle injury and chronic inflammation. Magnetic resonance imaging shows promise as surrogate outcome measure both DMD rehabilitation medicine that is capable predicting clinical benefit years advance functional measures. The mdx mouse reproduces the deficiency causes routinely used for preclinical drug testing. There need to develop sensitive, non-invasive measures model can be...
Factors that regulate synaptic specificity were investigated with Aplysia buccal and bag cell neurons in primary culture. In the presence of fetal calf serum electrical synapses are formed between buccal-buccal or bag-bag pairs, but not buccal-bag pairs. Instead, make inhibitory chemical on cells. However, nanomolar concentrations lectin concanavalin A this pattern changes, such more than 75 percent pairs exhibit frequency occurrence is reduced. Such changes may be important determining...
Abstract The study of inborn genetic errors can lend insight into mechanisms normal human development and congenital malformations. Here, we present the first detailed comparison cranial neuro pathology in two exceedingly rare individuals with cyclopia alobar holoprosencephaly ( HPE ) presence absence aberrant chromosome 18 a C h18). h18 fetus contained one Ch18 pseudo‐isodicentric duplication 18q partial deletion 18p from 18p11.31 where gene, TGIF , resides, to p terminus. In addition...
Abstract Prostate specific membrane antigen (PSMA) is overexpressed on prostate tumor cells and the neovascular endothelia various solid tumors. A bivalent immunotoxin generated by fusing a fold‐back single‐chain diabody derived from Fv fragments of an anti‐PSMA monoclonal antibody with truncated diphtheria toxin (DT) containing activity translocation domains [A‐dmDT390‐scfbDb(PSMA)] might be suitable for targeted therapy tumors that overexpress PSMA. In this study, PSMA‐positive...
Background Newly developed white matter ( WM ) injury is common after cardiopulmonary bypass CPB in severe/complex congenital heart disease. Fractional anisotropy FA allows measurement of macroscopic organization pathology but has rarely been applied . The aims our animal study were to define ‐induced alterations and determine correlations between these changes cellular events disease surgery. Methods Results Normal porcine development was first assessed 3 7 weeks age: 3‐week‐old piglets...
EGFR and EGFRvIII are overexpressed in various types of cancer, serving as optimal targets for cancer therapy. Capitalizing on the high specificity humanized antibody 806 (mAb806) to we designed generated a bivalent recombinant immunotoxin (RIT, DT390-BiscFv806) by fusing mAb806-derived single-chain variable fragment with diphtheria toxin fragment, DT390. In vitro, DT390-BiscFv806 efficiently internalized into cells exhibited cytotoxicity against U87 glioblastoma EGFRvIII-transfected...
Cystic fibrosis (CF) is an inherited, life-threatening disease caused by mutations in the gene encoding cystic transmembrane conductance regulator (CFTR), ABC transporter-class protein and ion channel that transports ions across epithelial cell membranes. The most common mutation leads to deletion of a single phenylalanine, resulting protein, F508del-CFTR, shows reduced trafficking membrane defective gating. ideal therapeutic approach would address both these defects restore function at same...