A5047902810- Cardiac Ischemia and Reperfusion
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Congenital heart defects research
- Biomedical Ethics and Regulation
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Cardiac Arrest and Resuscitation
- Fuel Cells and Related Materials
- Cardiac Fibrosis and Remodeling
- 3D Printing in Biomedical Research
- Cardiomyopathy and Myosin Studies
- Biomedical and Engineering Education
- Neuroscience and Neuropharmacology Research
- Cardiac Structural Anomalies and Repair
- Health and Medical Research Impacts
- Mechanical Circulatory Support Devices
- Cardiovascular Function and Risk Factors
- Electrospun Nanofibers in Biomedical Applications
- Pharmacogenetics and Drug Metabolism
- Computational Drug Discovery Methods
- Pancreatic function and diabetes
Mayo Clinic
2016-2025
Mayo Clinic in Florida
2015-2025
WinnMed
2014-2024
Mayo Clinic in Arizona
2015-2024
Center for Neuroscience and Regenerative Medicine
2011-2023
Io Therapeutics (United States)
2005-2021
University of Minnesota Rochester
1994-2021
Onze Lieve Vrouwziekenhuis Hospital
2013-2020
Clinical Orthopaedics and Related Research
2018-2019
Weatherford College
2018
Atrial fibrillation is a rhythm disorder characterized by chaotic electrical activity of cardiac atria. Predisposing to stroke and heart failure, this common condition increasingly recognized as heritable disorder. To identify genetic defects conferring disease susceptibility, patients with idiopathic atrial fibrillation, lacking traditional risk factors, were evaluated. Genomic DNA scanning revealed nonsense mutation in KCNA5 that encodes Kv1.5, voltage-gated potassium channel expressed...
ABSTRACT Members of the transforming growth factor pβ (TGF‐β) superfamily‐namely, TGF‐β and BMP2—applied to undifferentiated murine embryonic stem cells up‐regulated mRNA mesodermal (Brachyury) cardiac specific transcription factors (Nkx2.5, MEF2C). Embryoid bodies generated from primed with these demonstrated an increased potential for differentiation a significant increase in beating areas enhanced myofibrillogenesis. In environment postmitotic cardiomyocytes, engineered express...
Nuclear reprogramming provides an emerging strategy to produce embryo-independent pluripotent stem cells from somatic tissue. Induced (iPS) demonstrate aptitude for de novo cardiac differentiation, yet their potential heart disease therapy has not been tested.In this study, fibroblasts transduced with human stemness factors OCT3/4, SOX2, KLF4, and c-MYC converted into embryonic cell-like phenotype demonstrated the ability spontaneously assimilate preimplantation host morula via diploid...
Nerve regeneration after injury is a critical medical issue. In previous work, we have developed an oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel incorporated with positive charges as promising nerve conduit. this study, introduced cross-linkable bonds to graphene oxide and carbon nanotube obtain the functionalized acrylate (GOa) poly(ethylene (CNTpega). An electrically conductive was then fabricated by covalently embedding GOa CNTpega within OPF through chemical cross-linking...
Muscle weakness and myopathy are observed in vitamin D deficiency chronic renal failure, where concentrations of the active D3 metabolite, 1α,25-dihydroxyvitamin (1α,25(OH)2D3), low. To evaluate mechanism action 1α,25(OH)2D3 skeletal muscle, we examined mitochondrial oxygen consumption, dynamics, biogenesis changes expression nuclear genes encoding proteins human muscle cells following treatment with 1α,25(OH)2D3. The consumption rate (OCR) increased 1α,25(OH)2D3-treated cells. Vitamin...
Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem have shown preliminary efficacy. The Congestive Heart Failure Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort.
1. Mitochondrial dysfunction, secondary to excessive accumulation of Ca2+, has been implicated in cardiac injury. We here examined the action potassium channel openers on mitochondrial Ca2+ homeostasis, as these cardioprotective ion modulators have recently shown target a ATP-sensitive K+ channel. 2. In isolated mitochondria, diazoxide and pinacidil decreased rate magnitude uptake into matrix with an IC50 65 128 microM, respectively. At all stages uptake, depolarized membrane thereby...
Discovered in the cardiac sarcolemma, ATP-sensitive K + (K ATP ) channels have more recently also been identified within inner mitochondrial membrane. Yet consequences of channel activation on function remain partially documented. Therefore, we isolated mitochondria from rat hearts and used openers to examine effect opening membrane potential, respiration, generation, Ca 2+ transport, matrix volume. From a potential −180 ± 15 mV, openers, pinacidil (100 μM), cromakalim (25 levcromakalim (20...
Reaction to stress requires feedback adaptation of cellular functions secure a response without distress, but the molecular order this process is only partially understood. Here, we report previously unrecognized regulatory element in general syndrome. Kir6.2, ion-conducting subunit metabolically responsive ATP-sensitive potassium (K ATP ) channel, was mandatory for optimal capacity under stress. Genetic deletion Kir6.2 disrupted K channel-dependent adjustment membrane excitability and...