A5069329638- Graphene and Nanomaterials Applications
- Cardiomyopathy and Myosin Studies
- Bone Tissue Engineering Materials
- Muscle Physiology and Disorders
- Cardiac Ischemia and Reperfusion
- Ion channel regulation and function
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Biotin and Related Studies
- Aquaculture disease management and microbiota
- Viral Infectious Diseases and Gene Expression in Insects
- Fuel Cells and Related Materials
- Optical Coherence Tomography Applications
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Mosquito-borne diseases and control
- Fungal and yeast genetics research
- Mesenchymal stem cell research
- Cardiac electrophysiology and arrhythmias
- Photoacoustic and Ultrasonic Imaging
- Adipose Tissue and Metabolism
- Viral Infections and Vectors
- Cardiac Fibrosis and Remodeling
- Neuroscience and Neuropharmacology Research
Mayo Clinic
2014-2024
Mayo Clinic in Arizona
2013-2024
WinnMed
2016-2024
Mayo Clinic in Florida
2022
Kyungpook National University
2018-2019
Advanced Institute of Convergence Technology
2018
Digestive Health Research Institute
2017
Moffitt Cancer Center
2004
University of South Florida
2004
Chung-Ang University
2004
Atrial fibrillation is a rhythm disorder characterized by chaotic electrical activity of cardiac atria. Predisposing to stroke and heart failure, this common condition increasingly recognized as heritable disorder. To identify genetic defects conferring disease susceptibility, patients with idiopathic atrial fibrillation, lacking traditional risk factors, were evaluated. Genomic DNA scanning revealed nonsense mutation in KCNA5 that encodes Kv1.5, voltage-gated potassium channel expressed...
Nerve regeneration after injury is a critical medical issue. In previous work, we have developed an oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel incorporated with positive charges as promising nerve conduit. this study, introduced cross-linkable bonds to graphene oxide and carbon nanotube obtain the functionalized acrylate (GOa) poly(ethylene (CNTpega). An electrically conductive was then fabricated by covalently embedding GOa CNTpega within OPF through chemical cross-linking...
Friedreich ataxia is a severe autosomal-recessive disease characterized by neurodegeneration, cardiomyopathy and diabetes, resulting from reduced synthesis of the mitochondrial protein frataxin. Although frataxin ubiquitously expressed, deficiency leads to selective loss dorsal root ganglia neurons, cardiomyocytes pancreatic beta cells. How normally promotes survival these particular cells subject intense debate. The predominant view that sustains energy production other cellular functions...
Two-dimensional (2D) materials have emerged as a new promising research topic for tissue engineering because of their ability to alter the surface properties scaffolds and thus improve biocompatibility cell affinity. Multiple 2D materials, such graphene oxide (GO), been widely reported enhance adhesion proliferation. Recently, newly black phosphorus (BP) material has attracted attention in biomedical applications its unique mechanical electrochemical characteristics. In this study, we...
Biomimetic biomaterials require good biocompatibility and bioactivity to serve as appropriate scaffolds for tissue engineering applications.
Scaffold-free spheroids offer great potential as a direct supply of cells for bottom-up bone tissue engineering. However, the building functional with both and bioactive signals remains challenging. Here, we engineered mesenchymal stem (MSCs) two-dimensional heteronano-layers (2DHNL) that consisted black phosphorus (BP) graphene oxide (GO) to create 3D cell-instructive microenvironment large defect repair. The effects 2D materials on proliferation, osteogenic differentiation was evaluated in...
Three-dimensional (3D) printing technology is driving forward the progresses of various engineering fields, including tissue engineering. However, pristine 3D-printed scaffolds usually lack robust functions in stimulating desired activity for varied regeneration applications. In this study, we combined two-dimensional (2D) hetero-nanostructures and immuno-regulative interleukin-4 (IL-4) cytokines functionalization to achieve a pro-healing immuno-microenvironment optimized bone injury repair....
We have investigated the mechanism of frataxin, a conserved mitochondrial protein involved in iron metabolism and neurodegenerative disease. Previous studies revealed that yeast frataxin homologue (mYfh1p) is activated by Fe(II) presence O2 assembles stepwise into 48-subunit multimer (α48) sequesters >2000 atoms 2–4-nm cores structurally similar to ferritin cores. Here we show mYfh1p assembly driven two sequential oxidation reactions: A ferroxidase reaction catalyzed induces first step (α →...
Healing of nerve injuries is a critical medical issue. Biodegradable polymeric conduits are promising therapeutic solution to provide guidance for axon growth in given space, thus helping heal. Extensive studies the past decade reported that conductive materials could effectively increase neurite and extension vitro regeneration vivo. In this study, graphene oxide carbon nanotubes were covalently functionalized with double bonds obtain crosslinkable acrylate (GOa) sheets nanotube...
Abstract Conduits that promote nerve regeneration are currently of great medical concern, particularly when gaps exist between endings. To address this issue, our laboratory previously developed a conduit from biodegradable poly(caprolactone fumarate) (PCLF) supports peripheral regeneration. The present study improves upon work by further developing an electrically conductive, positively charged PCLF scaffold through the incorporation graphene, carbon nanotubes (CNTs), and...
Mitochondrial function depends on a continuous supply of iron to the iron-sulfur cluster (ISC) and heme biosynthetic pathways as well ability prevent iron-catalyzed oxidative damage. The mitochondrial protein frataxin plays key role in these processes by novel mechanism that remains be fully elucidated. Recombinant yeast human are able self-associate large molecular assemblies bind store ferrihydrite mineral. Moreover, either single monomers or polymers have been shown serve donors Fe(II)...
Frataxin is required for maintenance of normal mitochondrial iron levels and respiration. The mature form yeast frataxin (mYfh1p) assembles stepwise into a multimer 840 kDa (alpha(48)) that accumulates in water-soluble form. Here, two distinct oxidation reactions are shown to take place during the initial assembly step (alpha --> alpha(3)). A ferroxidase reaction with stoichiometry 2 Fe(II)/O(2) detected at Fe(II)/mYfh1p ratios < or = 0.5. Ferroxidation progressively overcome by autoxidation...
Urinary incontinence afflicts up to 40% of adult women in the United States. Stress urinary (SUI) accounts for approximately one-third these cases, precipitating ~200,000 surgical procedures annually. Continence is maintained through interplay sub-urethral support and urethral sphincter coaptation, particularly during activities that increase intra-abdominal pressure. Currently, correction SUI focuses on re-establishment support. However, mesh-based repairs are associated with foreign body...
Corneal transplantation by full-thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased corneas. Although corneal has high success rate, shortage of high-quality considerable limitation. Therefore, bioengineered corneas could be an effective solution this limitation, and decellularized extracellular matrix comprises promising scaffold their fabrication. In study, three-dimensional bioprinted collagen sheets were implanted into the...
Abstract Background With the enormous increment of globalization and global warming, it is expected that number newly evolved infectious diseases will continue to increase. To prevent damage due these infections, development a diagnostic method for detecting virus with high sensitivity in short time highly desired. In this study, we have developed disposable electrode high-sensitivity accuracy evaluate its performances several target viruses. Results Conductive silicon rubber (CSR) was used...
Nucleotide binding domains (NBDs) secure ATP-binding cassette (ABC) transporter function. Distinct from traditional ABC transporters, ABCC9-encoded sulfonylurea receptors (SUR2A) form, with Kir6.2 potassium channels, ATP-sensitive K+ (KATP) channel complexes. SUR2A contains ATPase activity harbored within NBD2 and, to a lesser degree, NBD1, catalytically driven conformations exerting determinate linkage on the pore. While homodomain interactions typify NBDs of conventional heterodomain NBD...
Acetyl‐CoA carboxylase 2 (ACC2) is an isoform of ACC functioning as a negative regulator fatty acid β‐oxidation. Spot14, thyroid hormone responsive protein, and Mig12, Spot14 paralog, have recently been identified regulators synthesis targeting ACC1, distinctive subtype ACC. Here, we examined whether Spot14/Mig12 modulates ACC2. Nanoscale protein topography mapped putative protein–protein interactions between purified human ACC2, validated by functional assays. Human ACC2 displayed...