A5037472900- Mitochondrial Function and Pathology
- Extracellular vesicles in disease
- Cardiac Structural Anomalies and Repair
- Mechanical Circulatory Support Devices
- Cardiac Valve Diseases and Treatments
- Genetic Neurodegenerative Diseases
- Coronary Interventions and Diagnostics
- Pharmaceutical studies and practices
- Muscle Physiology and Disorders
- Cardiovascular Health and Disease Prevention
- Periodontal Regeneration and Treatments
- Signaling Pathways in Disease
- Wound Healing and Treatments
- Vascular Procedures and Complications
- Cancer Mechanisms and Therapy
- Congenital Heart Disease Studies
- Angiogenesis and VEGF in Cancer
- Hernia repair and management
- Brain Metastases and Treatment
- Migraine and Headache Studies
- Cardiac pacing and defibrillation studies
- Acute Myocardial Infarction Research
- Endometriosis Research and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cardiac Fibrosis and Remodeling
Center for Neuroscience and Regenerative Medicine
2024
Mayo Clinic
2022-2024
Mayo Clinic in Arizona
2021-2024
WinnMed
2024
University of Florida
2015-2019
Florida College
2018-2019
Medosome Biotec (United States)
2019
Barth syndrome (BTHS) is a rare mitochondrial disease that affects heart and skeletal muscle has no curative treatment. It caused by recessive mutations in the X-linked gene TAZ, which encodes tafazzin. To develop clinically relevant therapy to restore tafazzin function treat BTHS, three different adeno-associated virus serotype 9 vectors were tested compared identify optimal promoter-cytomegalovirus (CMV), desmin (Des), or native promoter (Taz)-for TAZ expression following intravenous...
The purpose of this study was to evaluate left ventricular (LV) unloading strategies in patients supported with peripheral venoarterial extracorporeal membrane oxygenation (VA-ECMO). A retrospective review conducted all consecutive requiring VA-ECMO support for any indication, who underwent novel LV either direct atrial (LAVA) cannulation or pulmonary artery (PAVA) venting, comparison Impella and intra-aortic balloon pump (IABP). primary outcome successful bridge transplant, assist device,...
Urinary incontinence afflicts up to 40% of adult women in the United States. Stress urinary (SUI) accounts for approximately one-third these cases, precipitating ~200,000 surgical procedures annually. Continence is maintained through interplay sub-urethral support and urethral sphincter coaptation, particularly during activities that increase intra-abdominal pressure. Currently, correction SUI focuses on re-establishment support. However, mesh-based repairs are associated with foreign body...
The purpose of this study was to investigate a therapeutic approach targeting the inflammatory response and consequent remodeling from ischemic myocardial injury.
Aim: To investigate the regenerative effects of a platelet-derived purified exosome product (PEP) on human endometrial cells. Materials & methods: Endometrial adenocarcinoma cells (HEC-1A), stromal (T HESC) and menstrual blood-derived stem (MenSC) were assessed for absorption subsequent changes in cell proliferation wound healing properties over 48 h. Results: Cell increased PEP treated T HESC (p < 0.0001) MenSC 0.001) after 6 h HEC-1A 0.01) 12 improved between 24 36 0.05). Conclusion: was...
Next-generation sequencing is commonly used to screen for pathogenic mutations in families with Mendelian disorders, but due the pace of discoveries, gaps have widened some diseases between genetic and pathophysiological knowledge. We recruited analyzed 16 limb-girdle muscular dystrophy (LGMD) Arab descent from Saudi Arabia Sudan who did not confirmed diagnoses. The analysis included both traditional next-generation approaches. Cellular metabolic studies were performed on Pyroxd1 siRNA C2C12...
MEGF10 myopathy is a rare inherited muscle disease that named after the causative gene, MEGF10. The classic phenotype, early onset myopathy, areflexia, respiratory distress and dysphagia, severe immediately life-threatening. There are no disease-modifying therapies. We performed small molecule screen follow-up studies to seek novel therapy. A primary in vitro drug assessed cellular proliferation patterns Megf10-deficient myoblasts. Secondary evaluations were on hits using myoblasts derived...
The purpose of this study was to evaluate the biodistribution a platelet-derived exosome product (PEP), previously shown promote regeneration in setting wound healing, porcine model delivered through various approaches. Exosomes were labeled with DiR far-red lipophilic dye track and quantify exosomes tissue, following delivery via intravenous, pulmonary artery balloon catheter, or nebulization sus scrofa domestic pigs. Following euthanasia, detected by Xenogen IVUS imaging, while exosomal...
Cockayne Syndrome (CS) is caused by mutations in several genes that encode proteins involved DNA repair. These include traditional CS (CSA/CSB), as well Xeroderma Pigmentosum (XP) (including XPG). While characterized neurodegeneration and other clinical manifestations result an overall phenotype of premature aging, the typical XP photosensitivity skin cancer susceptibility. However, XPG presents with one two different phenotypes, depending on individual mutations: alone or + (XP/CS)....
Cockayne Syndrome (CS) is a rare, autosomal recessive, neurodegenerative disorder characterized by deficiencies which all contribute to an overall phenotype of premature aging. The underlying cause defect in genes involving DNA repair mechanisms. This includes CSA and CSB as well several associated with Xeroderma Pigmentosum (XP). Specifically, Group G (XPG) two distinct clinical presentations: photosensitivity alone (XP) neurodegeneration (XP-CS). XPG's role endonuclease nucleotide excision...
Barth Syndrome (BTHS) is caused by a single gene mutation affecting mitochondria and resulting in dilated cardiomyopathy, growth delay, neutropenia severe exercise intolerance. This gene, Tafazzin (TAZ) located at Xq28, responsible for cardiolipin (CL) remodeling the inner mitochondrial membrane. Mutation/s TAZ result altered of CL increasing Monolysocardiolpin (MLCL): ratios. imbalance leads to abnormal cristae formation decreased oxidative phosphorylation ATP production energy metabolism....
Mitochondrial turnover is a key marker in the process of cellular senescence. In order to track mitochondrial rates various cell lines, an indicator necessary identify formation new mitochondria along with degradation old mitochondria. This measure critical for study disease processes which there up or down regulation rates. One dynamic tool this balance pTRE-Tight-MitoTimer plasmid. The MitoTimer transgene combines red fluorescent protein, dsRed, and human cytochrome c oxidase subunit VIII...
A 54-year-old woman presented with progressively worsening headache and diplopia. Five months earlier, she began to experience imbalance nonspecific dizziness, followed by intermittent tingling numbness over her right eyebrow, eyelid, cheekbone. For the next 2 months, suffered severe frontal headaches, subsequently transforming an unremitting dull bifrontal without classical migrainous features or positional exacerbation. She then noticed horizontal binocular diplopia a mild vertical...
Introduction: Patients with coronary artery disease (CAD) not amenable to revascularization are reported have a 3-year mortality >14%. Advances in treatment modalities including medical management, rotational atherectomy, balloon lithotripsy, and drug-eluting stents work for focal but poorly treat diffuse CAD. In porcine model of in-stent restenosis, we here evaluated whether pro-angiogenic platelet-derived exosome product (PEP) could be delivered into the perivascular space foster...
Coronary angiography is the gold standard for diagnosis and treatment of epicardial coronary artery disease. Further, it an essential tool conduction translational cardiac research on large animal models, in development novel therapies. While humans has exceedingly low mortality rate; pigs, procedural been reported as high 15%, likely due to unsuitable variations angiographic techniques. Porcine models are used resemblance function anatomy humans. However, guidelines delineating proper...
For patients in cardiogenic shock, percutaneous mechanical circulatory support devices (MCSD) have emerged as critical tool to improve hemodynamics and optimize end-organ perfusion; successfully bridging recovery, decision or transplantation. The most commonly utilized MCSD is the femoral intra-aortic balloon pump (IABP). However, access hampers patient's mobility, quality of life, capacity participate rehabilitation. advent axillary artery approach has served circumvent limitations...
Introduction: Platelets are heavily implicated in angiogenesis; however, the underlying mechanism remains relatively uncharacterized. The goal of this study was to evaluate angiogenic potency a platelet-derived regenerative exosome product (PEP) an effort develop novel treatment for peripheral arterial disease. Here, we evaluated mechanistic basis PEP angiogenesis and it’s use In Vivo rodent hind limb ischemia model. Methods/Results: Under Current Good Manufacturing Practices, conditioned...