A5030990250- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrospinal fluid and hydrocephalus
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Axon Guidance and Neuronal Signaling
- Fetal and Pediatric Neurological Disorders
- Traumatic Brain Injury Research
- RNA modifications and cancer
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Ferroptosis and cancer prognosis
- Intracerebral and Subarachnoid Hemorrhage Research
Mayo Clinic in Florida
2017-2025
Jacksonville College
2017-2025
WinnMed
2019-2025
Nemours Children's Clinic
2020-2024
Cancer Clinic
2024
Mayo Clinic in Arizona
2024
Neurological Surgery
2014-2023
Johns Hopkins Medicine
2009-2020
Johns Hopkins University
2010-2020
Jacksonville University
2019
The tyrosine kinase c-Met promotes the formation and malignant progression of multiple cancers. It is well known that hyperactivation increases tumorigenicity tumor cell resistance to DNA damaging agents, properties associated with tumor-initiating stem cells. However, a link between signaling and/or maintenance neoplastic cells has not been previously identified. Here, we show activated functional in glioblastoma (GBM) neurospheres enriched for expression/function correlates marker...
Current glioblastoma therapies are insufficient to prevent tumor recurrence and eventual death. Here, we describe a method treat malignant glioma by nonviral DNA delivery using biodegradable poly(β-amino ester)s (PBAEs), with focus on the brain initiating cells (BTICs), cell population believed be responsible for formation of new tumors resistance many conventional therapies. We show transfection efficacy >60% low biomaterial-mediated cytotoxicity in primary human BTICs vitro even when grown...
The ion transporter NKCC1 determines brain tumor cell migration by regulating the interplay between adhesion and growth factor signaling, is a potential therapeutic target to treat cancer.
Introduction Glioblastoma is the most common primary malignant brain tumor, and refractory to surgical resection, radiation, chemotherapy. Human mesenchymal stem cells (hMSC) may be harvested from bone marrow (BMSC) adipose (AMSC) tissue. These are a promising avenue of investigation for delivery adjuvant therapies. Despite extensive research into putative mechanisms tumor tropism MSCs, there remains no direct comparison efficacy specificity AMSC BMSC towards glioma. Methods Under an...
We show that pluripotent human cells can be differentiated within an elastomer chip into a microenvironment mimicking the brain parenchyma.
Abstract Glioblastoma (GBM) stem cells (GSC) are a subpopulation of tumor that display stem-like characteristics (stemness) and play unique roles in propagation, therapeutic resistance, recurrence. Therapeutic targets GSCs focus increasing interest to improve GBM therapy. Here we report the hyaluronan-mediated motility receptor (HMMR) is highly expressed tumors, where it supports self-renewal tumorigenic potential GSCs. HMMR silencing impairs GSC inhibits expression markers regulators....
Neurogenesis persists in the adult subventricular zone (SVZ) of mammalian brain. During aging, SVZ neurogenic capacity undergoes a progressive decline, which is attributed to decrease population neural stem cells (NSCs). However, behavior NSCs that remain aged brain not fully understood. Here we performed comparative ultrastructural study niche 2-month-old and 24-month-old male C57BL/6 mice, focusing on NSC population. Using thymidine-labeling, showed residual divide less frequently than...
Epidermal growth factor receptor (EGFR) signalling is a potent driver of glioblastoma, malignant and lethal form brain cancer. Disappointingly, inhibitors targeting tyrosine kinase activity are not clinically effective EGFR persists on the plasma membrane to maintain tumour invasiveness. Here we show that endolysosomal pH critical for sorting turnover. By functioning as leak pathway protons, Na+/H+ exchanger NHE9 limits luminal acidification circumvent turnover prolong downstream pathways...
Glioblastoma is the most common adult primary malignant intracranial cancer. It associated with poor outcomes because of its invasiveness and resistance to multimodal therapies. Human adipose-derived mesenchymal stem cells (hAMSC) are a potential treatment their tumor tropism, ease isolation, ability be engineered. In addition, bone morphogenetic protein 4 (BMP4) has tumor-suppressive effects on glioblastoma brain tumor-initiating (BTIC), but difficult deliver tumors. We sought engineer...
Abstract Adult human adipose-derived mesenchymal stem cells (hAMSCs) are multipotent cells, which abundant, easily collected, and bypass the ethical concerns that plague embryonic cells. Their utility accessibility have led to rapid development of clinical investigations explore their autologous allogeneic cellular-based regenerative potential, tissue preservation capabilities, anti-inflammatory properties, anticancer among others. hAMSCs typically cultured under ambient conditions with 21%...
Glioblastoma multiforme is a heterogeneous and infiltrative cancer with dismal prognosis. Studying the migratory behavior of tumor-derived cell populations can be informative, but it places high premium on precision in vitro methods relevance vivo conditions. In particular, analysis 2D migration may not reflect invasion into 3D extracellular matrices vivo. Here, we describe method that allows time-resolved studies primary single-cell resolution fibrillar surface closely mimics migration. We...
Abstract Glioblastoma (GBM) remains the most aggressive primary brain cancer in adults. Similar to other cancers, GBM cells undergo metabolic reprogramming promote proliferation and survival. Glycolytic inhibition is widely used target such reprogramming. However, stability of glycolytic unclear especially a hypoxic tumor microenvironment. In this study, it was determined that glucose-6–phosphatase (G6PC/G6Pase) expression elevated when compared with normal brain. Human-derived...
Glioblastoma (GBM) is the most aggressive primary brain tumor in adults. The mechanisms that confer GBM cells their invasive behavior are poorly understood. electroneutral Na+-K+-2Cl- co-transporter 1 (NKCC1) an important cell volume regulator participates migration. We have shown inhibition of NKCC1 leads to decreased migration, vitro and vivo. now report on role cytoskeletal dynamics. show display a significant decrease F-actin content upon knockdown (NKCC1-KD). To determine potential...
A dual-function microneedle for rapid, high-resolution, and quantitative 3D deep-brain imaging power-efficient tissue ablation.