A5102989843- DNA Repair Mechanisms
- Prenatal Screening and Diagnostics
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Chronic Lymphocytic Leukemia Research
- PARP inhibition in cancer therapy
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
- Ovarian cancer diagnosis and treatment
- HER2/EGFR in Cancer Research
- BRCA gene mutations in cancer
- Liver physiology and pathology
- Chronic Myeloid Leukemia Treatments
- Cancer-related Molecular Pathways
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sarcoma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
Oregon Health & Science University
2015-2024
OHSU Knight Cancer Institute
2010-2015
Cancer Institute (WIA)
2010-2015
Oregon Medical Research Center
2013
Health & Life (Taiwan)
2011
Fred Hutch Cancer Center
2006
Harvard University
2006
Dana-Farber Cancer Institute
2006
Portland VA Medical Center
1998
Abbott Northwestern Hospital
1994
Fanconi anemia (FA) is a rare autosomal recessive cancer susceptibility disorder characterized by cellular hypersensitivity to mitomycin C (MMC). Six FA genes have been cloned, but the gene or corresponding subtypes B and D1 remain unidentified. Here we show that cell lines derived from FA-B FA-D1 patients biallelic mutations in BRCA2 express truncated proteins. Functional complementation of fibroblasts with wild-type complementary DNA restores MMC resistance. Our results link six cloned...
Smad4 is a central mediator of TGF-β signaling, and its expression downregulated or lost at the malignant stage in several cancer types. In this study, we found that was frequently not only human head neck squamous cell carcinoma (HNSCC) lesions, but also grossly normal adjacent buccal mucosa. To gain insight into importance observation, generated mice which deleted epithelia (referred to herein as HN-Smad4–/– mice) they developed spontaneous HNSCC. Interestingly, both tissue HNSCC from...
Abstract About 2 per cent of specimens from chorionic villus sampling (CVS) analysed either on direct preparation cytotrophoblast cells or afterculture mesenchymal stroma reveal confined placental mosaicism (CPM), most commonly involving chromosomal trisomy. A significantly higher rate prenatal loss (22 cent) as well the presence intrauterine growth retardation (IUGR) has been reported among pregnancies with CPM. To evaluate more precisely effect these aneuploid cell lines to placenta fetal...
Following introduction of DNA interstrand cross-links (ICLs), mammalian cells display chromosome breakage or cell cycle delay with a 4N content.To further understand the nature delay, previously described as G 2 /M arrest, we developed protocol to generate ICLs during specific intervals cycle.Synchronous populations 1 , S, and were treated photoactivated 4-hydroxymethyl-4,5,8trimethylpsoralen (HMT) scored for normal passage into mitosis.In contrast what was found ionizing radiation,...
The chromosomes of 81 pediatric germ cell tumors (GCTs) were analyzed as part two clinical treatment trials, INT-0098 and INT-0097, conducted by the Children's Cancer Group. analysis chromosome results showed differences with respect to sex, age, tumor location, histology. Sixteen 17 benign teratomas infants children less than 4 years old from gonadal extragonadal locations chromosomally normal. Twenty-three malignant GCTs same age group endodermal sinus varied in their karyotypic findings....
Although there exists compelling genetic evidence for a homologous recombination-independent pathway repair of interstrand cross-links (ICLs) involving translesion synthesis (TLS), biochemical support this model is lacking. To identify DNA polymerases that may function in TLS past ICLs, oligodeoxynucleotides were synthesized containing site-specific ICLs which the linkage was between N(2)-guanines, similar to formed by mitomycin C and enals. Here, data are presented mammalian cell...
We previously showed that fusion between hepatocytes lacking a crucial liver enzyme, fumarylacetoacetate hydrolase (FAH), and wild-type blood cells resulted in hepatocyte reprogramming. FAH expression was restored hybrid and, upon vivo expansion, ameliorated the effects of deficiency. Here, we show fusion-derived polyploid can undergo ploidy reductions to generate daughter with one-half chromosomal content. Fusion hybrids are, by definition, at least tetraploid. demonstrate reduction diploid...
We examined the response of tattoo pigments treated with three commercially available lasers: Q-switched ruby, Q-Switched neodynium:yttrium,aluminum,garnet (Nd:YAG), and alexandrite. Tattoos applied to hairless guinea pigs aforementioned lasers were evaluated clinically, histologically, ultrastructurally. Clinical evaluation showed red brown, dark orange pigment responded best Nd:YAG laser (1064 nm). The alexandrite was most effective for removing blue green pigment, ruby purple violet (532...
Fanconi anemia (FA) is a rare inherited bone marrow failure syndrome (IBMFS). Affected individuals must be distinguished from relatives, patients with mosaicism identified, and other IBMFS classified as non-FA. The diagnostic feature of FA increased chromosomal breakage in blood lymphocytes cultured diepoxybutane or mitomycin C. Here, we sought method to uniquely identify mosaicism, using cells participants the National Cancer Institute cohort. Lymphocytes were treated C, metaphases scored...