A5034309002- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Hepatitis C virus research
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Treatments and Studies
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- CAR-T cell therapy research
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Cancer therapeutics and mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Virus-based gene therapy research
- DNA and Nucleic Acid Chemistry
- Congenital heart defects research
- Sarcoma Diagnosis and Treatment
- Advanced Neural Network Applications
Guangxi Medical University
2024-2025
University of Washington
2011-2024
CTI BioPharma (United States)
2024
Shaoxing People's Hospital
2024
Ruijin Hospital
2014-2024
Shanghai Jiao Tong University
2012-2024
Allen Institute for Cell Science
2020-2023
The University of Sydney
2020
Renji Hospital
2009-2020
Soochow University
2020
Understanding how a subset of expressed genes dictates cellular phenotype is considerable challenge owing to the large numbers molecules involved, their combinatorics and plethora behaviours that they determine
Significance Bloom syndrome is a rare human genetic disease characterized by proportional dwarfism, immunodeficiency, and an elevated risk of many different cancer types. We used RNA expression profiling to identify networks mRNAs microRNAs that are differentially expressed in cells from patients associated with cell proliferation, survival, molecular pathways promoting cancer. Altered mRNA was some cases strongly correlated the presence G4 motifs, which may form G-quadruplex targets bound...
The Werner syndrome (WS) is a prototypic adult Mendelian progeroid in which signs of premature aging are associated with genomic instability and an elevated risk cancer. WRN RECQ helicase protein binds unwinds G-quadruplex (G4) DNA substrates vitro, we identified significant enrichment G4 sequence motifs at the transcription start site 5′ ends first introns (false discovery rate < 0.001) genes down-regulated WS patient fibroblasts. This finding provides strong evidence that structures many...
Method5 June 2020Open Access Transparent process High-throughput, microscope-based sorting to dissect cellular heterogeneity Nicholas Hasle Department of Genome Sciences, University Washington, Seattle, WA, USA Search for more papers by this author Anthony Cooke Leica Microsystems, Buffalo Grove, IL, Sanjay Srivatsan Heather Huang orcid.org/0000-0002-3534-9814 Divisions Basic Sciences and Human Biology, Fred Hutchinson Cancer Research Center, Jason J Stephany Zachary Krieger Dana Jackson...
This study identified 35 new sites for targeted transgene insertion that have the potential to serve as human genomic "safe harbor" (SHS). SHS these sites, located on 16 chromosomes, including both arms of X chromosome, and existing AAVS1, hROSA26, CCR5 was assessed using eight different desirable, widely accepted criteria verifiable with data. Three representative newly chromosomes 2 4 were then experimentally validated by in vitro vivo cleavage-sensitivity tests, analyzed population-level...
Zebrafish regenerate fin rays following amputation through epimorphic regeneration, a process that has been proposed to involve the epithelial-to-mesenchymal transition (EMT). We performed single-cell RNA sequencing (scRNA-seq) elucidate osteoblastic transcriptional programs during zebrafish caudal regeneration. show osteoprogenitors are enriched with components associated EMT and its reverse, mesenchymal-to-epithelial (MET), provide evidence markers cdh11 twist2 co-expressed in...
Abstract Background & Aims Serum Golgi protein 73 ( GP 73) is a potential biomarker for fibrosis assessment. We aimed to develop an algorithm based on and liver stiffness LS ) further improvement of accuracy significant in patients with antiviral‐naïve chronic hepatitis B virus (HBV) infection. Methods Diagnostic evaluation development 73‐ was performed training cohort (n = 267) independent 133) validation. Results A stepwise increasing pattern serum observed across stages HBV...
We present GeoManip, a framework to enable generalist robots leverage essential conditions derived from object and part relationships, as geometric constraints, for robot manipulation. For example, cutting the carrot requires adhering constraint: blade of knife should be perpendicular carrot's direction. By interpreting these constraints through symbolic language representations translating them into low-level actions, GeoManip bridges gap between natural robotic execution, enabling greater...
Few-shot Semantic Segmentation (FSS) is a challenging task that utilizes limited support images to segment associated unseen objects in query images. However, recent FSS methods are observed perform worse, when enlarging the number of shots. As set enlarges, existing networks struggle concentrate on high-contributed supports and could easily be overwhelmed by low-contributed severely impair mask predictions. In this work, we study issue, called dilution, our goal recognize, select, preserve,...
Proteins from human liver carcinoma Huh7 cells, representing transformed and cultured primary fetal hepatocytes (HFH) HH4 hepatocytes, nontransformed were extracted processed for proteome analysis. stimulated cells (interferon-alpha treatment the HFH induction of hepatitis C virus [HCV] proteins cells) corresponding control labeled with light heavy cleavable ICAT reagents, respectively. The samples combined, trypsinized, subject to cation-exchange avidin-affinity chromatographies. resulting...
Transient elastography (TE) is accurate in staging fibrosis noninvasively. However, a reliable serum biomarker with comparable accuracy also important, especially when TE unreliable/unavailable. Therefore, we aimed to evaluate the diagnostic performance of Golgi protein 73 (GP73) for significant patients chronic HBV infection. A total 801 liver disease (CLD; 492 infection and 309 non-HBV disease) biopsy were enrolled. Healthy controls (n = 180) hepatocellular carcinoma (HCC) 85) included...
Abstract Investigations on survival of patients with hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) are sparse and urgently needed. The current study aimed to evaluate the prognostic value circulating cell death biomarkers (M30-anigen, M65-antigen HMGB1) for HBV ACLF. In this prospective (2/2013–8/2014), 94 including 54 HBV-ACLF 40 chronic (CHB) were recruited. healthy controls (HC) also followed up 3 months short-term mortality. All three significantly elevated in...
Summary Despite the intimate link between cell organization and function, principles underlying intracellular relation organization, gene expression phenotype are not well understood. We address this by creating a benchmark for mean natural range of cell-to-cell variation. This can be used comparison to other normal or abnormal states. To do this, we developed reproducible microscope imaging pipeline generate high-quality dataset 3D, high-resolution images over 200,000 live cells from 25...
Hepatitis B virus (HBV) flare can occur in HBV patients either naïve or have interruption to treatment. Bacterial infection (BI) is a common complication of cirrhosis with potential severe outcomes. We aimed assess the impact on outcome HBV-related decompensated and BI.This was retrospective study from 2 tertiary academic hospitals Shanghai, China admitted developed BI during admission. The characteristics BI, prevalence flare, its organ failure, acute-on-chronic liver failure (ACLF) 90-day...
Bone and muscle are coupled through developmental, mechanical, paracrine, autocrine signals. Genetic variants at the CPED1-WNT16 locus dually associated with bone- muscle-related traits. While Wnt16 is necessary for bone mass strength, this fails to explain pleiotropy locus. Here, we show wnt16 required spine morphogenesis in zebrafish. In embryos, expressed dermomyotome developing notochord, contributes larval myotome morphology notochord elongation. Later, ventral midline of sheath,...