A5027076346- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Immune responses and vaccinations
- T-cell and B-cell Immunology
- Fungal Biology and Applications
- Cancer-related molecular mechanisms research
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Renal Diseases and Glomerulopathies
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- Respiratory viral infections research
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- Herbal Medicine Research Studies
- interferon and immune responses
- Conducting polymers and applications
Macau University of Science and Technology
2021-2025
Hong Kong Polytechnic University
2014-2024
UNSW Sydney
2024
University of Macau
2024
Pennine Acute Hospitals NHS Trust
2024
Sutherland Hospital
2024
Polytechnic University
2021
Guangxi University
2019
Tongji Hospital
2019
Huazhong University of Science and Technology
2019
Escherichia coli verotoxin (also known as Shiga-like toxin) has been implicated in the aetiology of hemolytic uremic syndrome and hemorrhagic colitis. The glycolipid binding specificity purified from E. H30 cloned bacteriophage H19B examined. Verotoxin both sources binds specifically to globotriosyl ceramide containing carbohydrate sequence galactose alpha 1-4galactose beta 1-4glucose-ceramide. Removal terminal or substitution with N-acetylgalactosamine 1-3 linkage deletes toxin activity. A...
Abstract The analysis of protein biomarkers is great importance in the diagnosis diseases. Although many convenient and low‐cost electrochemical approaches have been extensively investigated, they are not sensitive enough detection with low concentrations physiological environments. Here, this study reports a novel organic‐electrochemical‐transistor‐based biosensor that can successfully detect cancer ultrahigh sensitivity. devices operated by detecting activity on gate electrodes, which...
The outbreak of COVID-19 and its continued spread have seriously threatened public health. Antibody testing is essential for infection diagnosis, seroepidemiological analysis, vaccine evaluation. However, convenient, fast, accurate antibody detection remains a challenge in this protracted battle. Here, we report an ultrafast, low-cost, label-free, portable SARS-CoV-2 immunoglobulin G (IgG) platform based on organic electrochemical transistors (OECTs), which can be remotely controlled by...
Electrochemical transistors (ECTs) have shown broad applications in bioelectronics and neuromorphic devices due to their high transconductance, low working voltage, versatile device design. To further improve the performance, semiconductor materials with both carrier mobilities large capacitances electrolytes are needed. Here, we demonstrate ECTs based on highly oriented two-dimensional conjugated metal-organic frameworks (2D c-MOFs). The ion-conductive vertical nanopores formed within 2D...
Abstract Little is known about the innate immune response to severe acute respiratory syndrome (SARS) coronavirus (CoV) infection. Mannose-binding lectin (MBL), a key molecule in immunity, functions as an ante-antibody before specific antibody response. Here, we describe case-control study that included 569 patients with SARS and 1188 control subjects used vitro assays investigate role MBL plays SARS-CoV The distribution of gene polymorphisms was significantly different between subjects,...
The pig edema disease toxin has been shown by a tlc glycolipid binding assay to bind specifically globotetraosylceramide (Gb4, GalNAcβ1–3Galα1–4Galβ1–4GlcCer.). Binding was reduced for globotriosylceramide (Gb3, Galα1–4Galβ1–4GlcCer) and more markedly the Forssman antigen (GalNAcα1–3GalNAcβ1–3Galα1–4Galβ1–4GlcCer). Paragloboside, blood group A glycolipids, glycolipids terminating in Gal NAcβ1–4Gal-, which globoside present as an internal sequence did not toxin. Isogloboside...
Cell-free HIV-1 virions are poor stimulators of type I interferon (IFN) production. We examined here how HIV-infected cells recognized by plasmacytoid dendritic (pDCs) and other cells. show that infected lymphocytes more potent inducers IFN than virions. There target cell-type differences in the recognition lymphocytes. In primary pDCs pDC-like cells, occurs large part through TLR7, as demonstrated use inhibitors TLR7 silencing. Donor expressing replication-defective viruses, carrying...
Human coronaviruses are associated with upper respiratory tract infections that occasionally spread to the lungs and other organs. Although airway epithelial cells represent an important target for infection, epithelium is also composed of elaborate network dendritic (DCs) essential sentinels immune system, sensing pathogens presenting foreign antigens T lymphocytes. In this report, we show in vitro infection by human coronavirus 229E (HCoV-229E) induces massive cytopathic effects DCs,...
Influenza is an acute respiratory viral disease that transmitted in the first few days of infection. Evasion host innate immune defenses, including natural killer (NK) cells, important for virus's success as a pathogen humans and other animals. NK cells encounter influenza viruses within microenvironment infected are immunity during virus It therefore to investigate direct effects on cells. In this study, we demonstrated time directly infects replicates primary human Viral entry into was...
Summary Mesenchymal stem cells (MSCs) are an important cellular component of the bone marrow microenvironment for supporting haemopoiesis. However, their response to high‐dose chemotherapy remains unknown. We assessed acute direct effects individual chemotherapeutic agents on human MSCs (hMSCs). Using in vitro culture system, chemosensitivity hMSCs was determined by XTT (2,3‐bis(2‐methoxy‐4‐nitro‐5‐sulphophenyl)‐5‐[(phenylamino) carbonyl]‐2 H ‐tetrazolium hydroxide) assay comparison with...
Objective. To study the epidemiologic, clinical, laboratory, and radiologic features, prognostic indicators, short-term to medium-term outcomes for children with severe acute respiratory syndrome (SARS) validate performance characteristics of a clinical case definition, calculated respect SARS-associated coronavirus (SARS-CoV) seroconversion. Methods. Children <18 years age, from single-site outbreak, who satisfied definition SARS, subsequent serologic confirmation, were treated...
To comprehensively evaluate the effect of a short-term diabetes self-management education (DSME) on metabolic markers and atherosclerotic parameters in patients with type 2 diabetes. 76 were recruited this study. They divided into intervention group (n = 36) control 40). The received 3-month intervention, including an 8-week mellitus subsequent 4 weeks practice guidelines. standard advice medical nutrition therapy. Metabolic markers, carotid intima-media thickness (CIMT), arterial stiffness...
Abstract Background Cytokines play important roles in antiviral action. We examined whether polymorphisms of IFN-γ , TNF-α and IL-10 affect the susceptibility to outcome severe acute respiratory syndrome (SARS). Methods A case-control study was carried out 476 Chinese SARS patients 449 healthy controls. tested for their associations with SARS. Results +874A allele associated a dose-dependent manner ( P < 0.001). Individuals +874 AA AT genotype had 5.19-fold (95% Confidence Interval [CI],...
Polysaccharides derived from fungi and plants have been increasingly used as dietary supplement with therapeutic intention for cancer. However, whether these polysaccharides different sources structures can elicit similar immunological effects remain unknown. This study aims to investigate compare the of selected groups purified crude on human dendritic cells (DCs), most potent antigen-presenting cells. The were Ganoderma lucidum [(GL) Lingzhi, Reishi], a medicinal mushroom commonly by...
In 1997, avian influenza virus H5N1 was transmitted directly from chicken to human and resulted in a severe disease that had higher mortality rate adults than children. The characteristic mononuclear leukocyte infiltration the lung high inflammatory response infection prompted us compare chemokine responses between virus-infected adult neonatal monocyte-derived macrophages (MDMs). effects of A/Hong Kong/483/97 (H5N1) (H5N1/97), its precursor A/Quail/Hong Kong/G1/97 (H9N2) (H9N2/G1),...
Human mesenchymal stromal cells (MSCs, also known as stem cells) are multipotent with potential therapeutic value. Owing to their osteogenic capability, MSCs may be clinically applied for facilitating osseointegration in dental implants or orthopedic repair of bony defect. However, whether wound infection oral microflora interfere the growth and differentiation human remains unknown. This study investigated proliferation would affected by potent gram-positive gram-negative derived bacterial...
Recent genome-wide association studies have revealed numerous genetic associations between specific single-nucleotide polymorphisms (SNPs) and immune-mediated inflammatory diseases. The current challenge is to identify of the variants with effector mechanisms implicated in pathogenesis. This study was undertaken investigate link variation at loci associated spondyloarthritis (SpA) function CD4+ T lymphocyte subsets involved chronic disease.Expression Th17 Th1 cytokines transcription factors...