A5043194263- Prion Diseases and Protein Misfolding
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Salmonella and Campylobacter epidemiology
- Coccidia and coccidiosis research
- Toxoplasma gondii Research Studies
- Health, Medicine and Society
- Animal Nutrition and Physiology
- Herpesvirus Infections and Treatments
- Milk Quality and Mastitis in Dairy Cows
- Diagnosis and treatment of tuberculosis
- Parasitic Infections and Diagnostics
- Veterinary Equine Medical Research
- Animal Disease Management and Epidemiology
- Animal Genetics and Reproduction
- Neurological diseases and metabolism
- Hepatitis Viruses Studies and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Vibrio bacteria research studies
- Microbial infections and disease research
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Immune responses and vaccinations
- Tuberculosis Research and Epidemiology
- Rabbits: Nutrition, Reproduction, Health
Université de Tours
2013-2025
Centre Val de Loire
2024-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Infectiologie Animale et Santé Publique
2014-2023
Institut National de la Recherche Agronomique
2015
l'Assurance Maladie
1995
Maternité Port Royal
1993
Infectious mastitis cuts down milk production profitability and is a major animal welfare problem. Bacteria-induced inflammation in the mammary gland (MG) driven by innate immunity, but adaptive immunity can modulate response. Several studies have shown that it possible to elicit MG sensitization an antigen subsequently infused into lumen of gland. The objective our study was characterize triggered cows sensitized ovalbumin, identifying cytokines chemokines likely play part reaction. Among...
Coccidiosis, a disease caused by intestinal apicomplexan parasites Eimeria, is threat to poultry production. Eimeria tenella one of the most pathogenic species, frequently causing high prevalence opportunistic infections.The objective this study investigate role microbiota in pathogenesis severe infection.We have previously shown that can promote parasite development. To effect on infection, we used an experimental condition (inoculum 10 000 oocysts E. INRAE) which load similar between...
Abstract The cytokine IL-17A has been shown to play critical roles in host defense against bacterial and fungal infections at different epithelial sites, but its role the of mammary gland (MG) seldom investigated, although MG constitute main pathology afflicting dairy cows. In this study, we showed that contributes Escherichia coli infection by using a mouse mastitis model. After inoculation with mastitis-causing E. strain, load increased rapidly, triggering an intense influx leukocytes into...
Coccidiosis is a widespread intestinal disease of poultry caused by parasite the genus Eimeria. Eimeria tenella, one most virulent species that specifically colonizes caeca, an organ which harbors rich and complex microbiota. Our objective was to study impact microbiota on infection development using original model germ-free broilers. We observed chickens presented significantly much lower load oocysts in caecal contents than conventional chickens. This decrease measurable tissue RT-qPCR at...
<title>Abstract</title> Over the past few years, animal welfare has taken more place in society and especially scientific research. It become necessary to refine experimental procedures as much possible infectiology our reference model, toxoplasmosis, agreement with 3Rs rule different ethical concerns. Thus, establishment of a treatment using analgesics would relieve animals acutely infected <italic>Toxoplasma gondii</italic>. However, use this analgesic should no way alter pathophysiology...
The effectiveness of intramuscular vaccines aimed at preventing severe COVID-19 remains limited due to waning immunity and the emergence novel variants. Next-generation are needed for broader protection blocking virus transmission. Here, we rationally designed an original nasal subunit vaccine composed a fusion protein (SwFN) made Wuhan spike nucleoprotein combined with biocompatible mucosal nanocarriers (Nc). In mouse model, Nc-SwFN elicited multivalent serum neutralizing antibodies. Robust...
From the registry of self employed workers living in Paris, a group 105 dental technicians was studied to evaluate occupational exposure, determine respiratory manifestations, and investigate immune disturbances. Seventy one (age range 43-68: D), 34 younger than 43 or older 68 (group d), control 43-66: C) were investigated. The demographic characteristics smoking habits groups D C did not differ significantly. often worked alone (43.7%) small laboratories without adequate dust control. mean...
The main features of lung infection and inflammation are a massive recruitment neutrophils the subsequent release neutrophil serine proteases (NSPs). Anti-infectious and/or anti-inflammatory treatments must be tested on suitable animal model. Mice models do not replicate several aspects human disease. This is particularly true for cystic fibrosis (CF), which has led scientific community to search new models. We have shown that mice appropriate characterizing drugs targeting...
Excessive lung inflammation and airway epithelial damage are hallmarks of human inflammatory diseases, such as cystic fibrosis (CF). Enhancement innate immunity provides protection against pathogens while reducing lung-damaging inflammation. However, the mechanisms underlying immunity-mediated in remain mysterious, part because lack appropriate animal models for these diseases. TLR5 (Toll-like receptor 5) stimulation by its specific ligand, bacterial protein flagellin, has been proposed to...
Achieving the control of bovine tuberculosis (bTB) would require discovery an efficient combined immunodiagnostic and vaccine strategy. Since in vivo experiments on cattle are not ethically economically acceptable there is a need for cost-effective animal model capable reproducing, as closely possible, physiopathology bTB to (i) better characterize cellular molecular features immunopathogenesis (ii) screen preclinical candidates. To develop such model, we focused C3HeB/FeJ Kramnik's mouse...
Transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases affecting wide range mammalian species. They caused by prions, proteinaceous pathogen essentially composed PrPSc, an abnormal isoform the host encoded cellular prion protein PrPC. Constrained steric interactions between PrPSc and PrPC thought to provide prions with species specificity, control cross-species transmission into other populations, including humans. Transgenetic expression foreign PrP genes...
Breeding towards genetic resistance to prion disease is effective in eliminating scrapie. In sheep, classical forms of scrapie have been eradicated almost completely several countries by breeding programs using a protein (PrP) gene (PRNP) amino acid polymorphism. For goats, field and experimental studies provided evidence for polymorphisms that are associated with scrapie, but only limited data available concerning the susceptibility caprine PRNP genotypes BSE. this study, goat kids...
Enteric infections caused by Chlamydia (C.) psittaci are frequent in ducks, but mostly remain subclinical under field conditions. To emulate natural infection, we investigated the pathogenic potential of a C. strain orally inoculated 4-day-old ducklings. Three different challenge doses were tested and seven contact animals also mock-inoculated with buffer each group. Over course ten days, birds monitored for clinical symptoms chlamydial dissemination before final examination tissues using...
Entamoeba histolytica is an important parasite of the human intestine. Its life cycle monoxenous with two stages: (i) trophozoite, growing in intestine and (ii) cyst corresponding to dissemination stage. The trophozoite can live as a commensal leading asymptomatic infection or tissue invasive form producing mucosal ulcers liver abscesses. There no animal model mimicking whole disease cycle. Most biological information on E. has been obtained from adapted axenic culture. reproduction...
Clostridioides difficile infection (CDI) is a serious healthcare-associated disease, causing symptoms such as diarrhea and pseudomembranous colitis. The major virulence factors responsible for the disease are two secreted cytotoxic proteins, TcdA TcdB. A parenteral vaccine based on formaldehyde-inactivated TcdB supplemented with alum adjuvant, has previously been investigated in humans but resulted an insufficient immune response. In search improved response, we novel toxin inactivation...
Both the quality of chicken meat and chicks are influenced by level breast muscle glycogen reserves. In order to study role digestive metabolism in establishing this muscular phenotype, we compared two divergent lines for ultimate pH (pHu) meat, a proxy Males aged 4 weeks had twice content pHu- line (low pHu) than pHu + (high pHu). The increase reserves (pHu-) was associated with higher relative weight proventriculus gizzard, as well better apparent ileal digestibility nitrogen calcium....
Background: Hepatitis E virus (HEV) is a zoonotic transmitted by pig meat and responsible for chronic hepatitis in immunocompromised patients. It has proved challenging to reproduce this disease its natural reservoir. We therefore aimed develop model of improve the characterization disease. Methods: Ten pigs were treated with tacrolimus-based regimen intravenously inoculated HEV. Tacrolimus trough concentration, HEV viremia, viral diversity, innate immune responses, liver histology, clinical...
Poultry are the main source of human infection by Salmonella . As infected poultry asymptomatic, identifying farms is difficult, thus controlling animal infections primary importance. cell tropism known to govern disease, our aim was therefore identify host–cell types in organs chicks be involved and investigate role three invasion factors this process (T3SS-1, Rck PagN). Chicks were inoculated with wild-type or isogenic fluorescent Typhimurium mutants via intracoelomic route. Our results...
Abstract Background Mammary gland (MG) infections (mastitis) are frequent diseases of dairy cows that affect milk quality, animal welfare and farming profitability. These commonly associated with the bacteria Escherichia coli Staphylococcus aureus . Different in vitro models have been used to investigate early response MG bacteria, but role teat mastitis pathogenesis has received less attention. In this study, we punch-excised tissue as an ex vivo model study immune mechanisms arise during...
Poultry production is an important agricultural sector for human food worldwide. Chicks after hatch often face health problems leading to economic losses that are deleterious breeders. Avian defensin 2 (AvBD2) a prominent host defense peptide of the intestinal mucosa cecum and involved in resistance poultry bacterial pathogens. This could thus represent innate immunity marker robustness birds. To test this hypothesis by comparing fast-growing slow-growing lines different conditions breeding,...
Abstract Poultry are the main source of human infection by Salmonella . As infected poultry asymptomatic, identification farms is difficult. Controlling animal infections thus primary importance. cell tropism known to govern disease, our aim was therefore identify host-cell types in chicks and role three invasion factors this process (T3SS-1, Rck PagN). Chicks were inoculated with wild-type or isogenic fluorescent Typhimurium mutants via intraperitoneal route. Our results show that liver,...