A5073733069- Advanced Electron Microscopy Techniques and Applications
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Electron and X-Ray Spectroscopy Techniques
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Proteins in Food Systems
- Peptidase Inhibition and Analysis
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Advanced X-ray Imaging Techniques
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Polysaccharides Composition and Applications
- Animal Virus Infections Studies
- Hippo pathway signaling and YAP/TAZ
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Fecal contamination and water quality
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Lipid metabolism and disorders
- Bacterial biofilms and quorum sensing
The University of Melbourne
2018-2025
Walter and Eliza Hall Institute of Medical Research
2022-2025
The University of Tokyo
2019
Australian Centre for Disease Preparedness
2013-2017
Commonwealth Scientific and Industrial Research Organisation
2010-2017
Animal, Food and Health Sciences
2013-2014
CSL (Switzerland)
2013
Behringwerke (Germany)
2013
CSL (Australia)
2013
CSL (United States)
2013
The cell walls of mycobacteria form an exceptional permeability barrier, and they are essential for virulence. They contain extractable lipids long-chain mycolic acids that covalently linked to peptidoglycan via arabinogalactan network. were thought asymmetrical bilayer considerable thickness, but this could never be proven directly by microscopy or other means. Cryo-electron tomography unperturbed detergent-treated cells Mycobacterium smegmatis embedded in vitreous ice now reveals the...
Objective— The ability of high-density lipoprotein (HDL) to remove cholesterol from atherosclerotic plaque is thought underlie its inverse correlation with cardiovascular risk. Our objective was produce and characterize a human apolipoprotein AI (apoA-I) product optimized treat clinical disease. Approach Results— A new formulation full length, plasma-derived apoA-I termed CSL112 designed maximize the efflux cells exhibit favorable pharmacological properties. disc-shaped particle that...
The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind the outer surface, but little known about cellular components may associate with internal side microtubules. We used cryoelectron tomography investigate in a quantitative manner three dimensional structure microtubules intact mammalian cells. show lumen this native state filled discrete, globular particles diameter 7 nm and spacings between 8 20 neuronal Cross-sectional views confirm presence...
FANCI:FANCD2 monoubiquitination is a critical event for replication fork stabilization by the Fanconi anemia (FA) DNA repair pathway. It has been proposed that at stalled forks, monoubiquitinated-FANCD2 serves to recruit proteins contain ubiquitin-binding motifs. Here, we have reconstituted FA pathway in vitro study functional consequences of monoubiquitination. We report does not promote any specific exogenous protein:protein interactions, but instead stabilizes heterodimers on dsDNA. This...
Microtubules are dynamic cytoskeletal structures important for cell division, polarity, and motility therefore major targets anticancer antiparasite drugs. In the invasive forms of apicomplexan parasites, which highly polarized often motile cells, exceptionally stable subpellicular microtubules determine shape parasite, serve as tracks vesicle transport. We used cryoelectron tomography to image cytoplasmic in three dimensions within intact, rapidly frozen Plasmodium sporozoites. This...
Abstract The necroptosis pathway is a lytic, pro-inflammatory mode of cell death that widely implicated in human disease, including renal, pulmonary, gut and skin inflammatory pathologies. precise mechanism the terminal steps pathway, where RIPK3 kinase phosphorylates triggers conformation change oligomerization effector, MLKL, are only emerging. Here, we structurally identify RIPK3-mediated phosphorylation MLKL activation loop as cue for pseudokinase domain dimerization. dimerization...
Interleukin (IL-)11, an IL-6 family cytokine, has pivotal roles in autoimmune diseases, fibrotic complications, and solid cancers. Despite intense therapeutic targeting efforts, structural understanding of IL-11 signalling mechanistic insights into current inhibitors are lacking. Here we present cryo-EM crystal structures the human complex, including complex containing complete extracellular domains shared β-receptor, gp130. We show that formation requires conformational reorganisation...
The ubiquitin kinase PINK1 accumulates on damaged mitochondria to trigger mitophagy, and loss-of-function mutations cause early onset Parkinson’s disease. Nucleotide analogs such as kinetin triphosphate (KTP) were reported enhance activity may represent a therapeutic strategy for the treatment of Here, we investigate interaction with nucleotides, including KTP. We establish cryo-EM platform exploiting dodecamer assembly Pediculus humanus corporis ( Ph ) determine structures bound AMP-PNP...
The interactions that occur during HIV Pr55Gag oligomerization and genomic RNA packaging are essential elements facilitate assembly. However, mechanistic details of these not clearly defined. Here, we overcome previous limitations in producing large quantities full-length recombinant is required for isothermal titration calorimetry (ITC) studies, have revealed the thermodynamic properties assembly first time. Thermodynamic analysis showed binding between an energetically favourable reaction...
Mutations in the ubiquitin kinase PINK1 cause early onset Parkinson’s Disease, but how is stabilized at depolarized mitochondrial translocase complexes has remained poorly understood. We determined a 3.1-Å resolution cryo-electron microscopy structure of dimeric human an endogenous array TOM and VDAC complexes. Symmetric arrangement two core around central VDAC2 dimer facilitated by TOM5 TOM20, both which also bind C-lobes. enters mitochondria through proximal TOM40 barrel complex, guided...
Invasion of erythrocytes by members the Plasmodium genus is an essential step parasite lifecycle, orchestrated numerous host-parasite interactions. In P. falciparum Rh5, with PfCyRPA, PfRipr, PfCSS, and PfPTRAMP, forms PCRCR complex which binds basigin on erythrocyte surface. Rh5 restricted to its close relatives; however, PTRAMP, CSS Ripr orthologs are present across genus. We investigated from three species elucidate common features complex. Like falciparum, PTRAMP form a disulfide-linked...
<title>Abstract</title> Invasion of erythrocytes by members the Plasmodium genus is an essential step parasite lifecycle, orchestrated numerous host-parasite interactions. In P. falciparum Rh5, with PfCyRPA, PfRipr, PfCSS, and PfPTRAMP, forms PCRCR complex which binds basigin on erythrocyte surface. Rh5 restricted to its close relatives; however, PTRAMP, CSS Ripr orthologs are present across genus. We investigated from three species elucidate common features complex. Like falciparum, PTRAMP...
ABSTRACT We describe a novel and noninvasive, microscopy-based method for visualizing the structure dynamics of microbial biofilms, individual fluorescent cells, inorganic colloids within model porous medium. Biofilms growing in flow cells packed with granules an amorphous fluoropolymer could be visualized as consequence refractive index matching between solid grains aqueous immersion In conjunction capabilities confocal microscopy nondestructive optical sectioning, use fluoropolymers matrix...