A5020748618- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Peptides and Activities
- Tuberculosis Research and Epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Vibrio bacteria research studies
- Sperm and Testicular Function
- Cystic Fibrosis Research Advances
- Pharmaceutical and Antibiotic Environmental Impacts
- Drug Transport and Resistance Mechanisms
- Antimicrobial agents and applications
- Epilepsy research and treatment
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Reproductive Biology and Fertility
- Bacterial biofilms and quorum sensing
- Peroxisome Proliferator-Activated Receptors
- Influenza Virus Research Studies
- Cancer therapeutics and mechanisms
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Inhalation and Respiratory Drug Delivery
- Antibiotic Use and Resistance
The University of Melbourne
2017-2024
Monash University
2015-2024
Australian Regenerative Medicine Institute
2015-2024
Discovery Institute
2024
RMIT University
2018-2022
Purdue University West Lafayette
2021
Faculty (United Kingdom)
2019
Molina Center for Energy and the Environment
2018
Northeast Agricultural University
2018
NAACP Legal Defense and Educational Fund
2009-2017
Based on its clinical benefits, Trikafta — the combination of folding correctors VX-661 (tezacaftor), VX-445 (elexacaftor), and gating potentiator VX-770 (ivacaftor) was FDA approved for treatment patients with cystic fibrosis (CF) carrying deletion phenylalanine at position 508 (F508del) CF transmembrane conductance regulator (CFTR) least 1 allele. Neither mechanism action nor susceptibility rare mutants to are known. Here, we show that, in human bronchial epithelial cells, synergistically...
Abstract The emergence of multidrug-resistant (MDR) Gram-negative pathogens is an urgent global medical challenge. old polymyxin lipopeptide antibiotics (polymyxin B and colistin) are often the only therapeutic option due to resistance all other classes lean antibiotic drug development pipeline. However, colistin suffer from major issues in safety (dose-limiting nephrotoxicity, acute toxicity), pharmacokinetics (poor exposure lungs) efficacy (negligible activity against pulmonary infections)...
Lipid peroxidation products such as the naturally occurring aldehyde 4-hydroxynonenal (4-HNE) are known to be cytotoxic toward different cell types, including spermatozoa. In order understand this at molecular level, we have employed a proteomic approach characterize direct 4-HNE adducts on human Several proteins were identified of particular interest, labeling histone methyltransferase and dynein heavy chain. addition, found that bound part activation segment, cysteine residue 199, protein...
Polymyxin B and colistin are currently used as a 'last-line' treatment for multidrug-resistant Gram-negative bacteria. However very little is known about the pharmacological differences between polymyxin B1, B2, A, B, major cyclic lipopeptides components present in products. Here, we report on vitro vivo antimicrobial activity toxicity of these lipopeptide components. All four had comparable MICs (<0.125-4 mg/L) against panel clinical isolates. They also (Δlog10 CFU/mL >-3) nephrotoxicity...
This is the first study to investigate hepatoprotective effect of CQ on acute liver injury caused by carbon tetrachloride (CCl4) in a murine model and underlying molecular mechanisms. Ninety-six mice were randomly divided into control (n = 8), CCl4 40), + 40) treatment groups. In group, intraperitoneally (i.p) injected with 0.3% (10 mL/kg, dissolved olive oil); i.p at 50 mg/kg 2, 24, 48 h before administration. The groups administered an equal vehicle or (50 mg/kg). Mice killed 6, 12, post...
Subcellular proteomics not only deepens our knowledge of what proteins are present within cells, but also opens understanding as to where those reside. Given the highly differentiated, cross‐linked state spermatozoa, such studies have proven difficult perform. In this study we fractionated spermatozoa into two components, consisting either head or flagellar region. Following SDS ‐ PAGE , 1 mm slices were digested and used for LC MS / analysis. total, 1429 identified with 721 being...
The antimicrobial lipopeptides polymyxin B and E (colistin) are being used as a 'last-line' therapy for infections caused by multidrug-resistant Gram-negative pathogens. Polymyxin resistance implies total lack of antibiotics the treatment life-threatening 'superbugs'. This report details structure-activity relationships (SAR) based design, in toto synthesis, preclinical evaluation series novel with better antibacterial activity against polymyxin-resistant bacteria.
The dry antibiotic development pipeline coupled with the emergence of multidrug resistant Gram-negative 'superbugs' has driven revival polymyxin lipopeptide antibiotics. Polymyxin resistance implies a total lack antibiotics for treatment life-threatening infections. molecular imaging probes that possess native polymyxin-like antibacterial activity is barrier to understanding mechanisms and new generation lipopeptides. Here we report regioselective modification B core scaffold at N-terminus...
Nephrotoxicity is the major dose-limiting factor for clinical use of colistin against multidrug-resistant (MDR) Gram-negative bacteria. This study aimed to investigate protective effect lycopene on colistin-induced nephrotoxicity in a mouse model. Fifty mice were randomly divided into 5 groups: control group (saline solution), (20 mg/kg body weight/day administered orally), (15 mg/kg/day intravenously), mg/kg/day) plus (5 group, and group; all treated 7 days. At 12 h after last dose, blood...
Bacterial resistance is among the most serious threats to human health globally, and many bacterial isolates have emerged that are resistant all antibiotics in monotherapy. Aminoglycosides often used combination therapies against severe infections by multidrug-resistant bacteria. However, models quantifying different antibacterial effects of aminoglycosides lacking. While mode aminoglycoside action on protein synthesis has been studied, their disruptive outer membrane Gram-negative bacteria...
This study investigates the protective effect of baicalein on carbon tetrachloride (CCl4)-induced acute liver injury and underlying molecular mechanisms. Mice were orally administrated at 25 100 mg/kg/day for 7 consecutive days or ferrostatin-1 (Fer-1) 10 mg/kg was i.p. injected in mice 2 24 h prior to CCl4 injection vehicle. Our results showed that Fer-1 supplementation significantly attenuated exposure-induced elevations serum alanine aminotransferase aspartate aminotransferase,...
Metal-organic framework (MOF)-based drug delivery nanomaterials for cancer therapy have attracted increasing attention in recent years. Here, an enhanced chemodynamic anti-tumor strategy by promoting the Fenton reaction using core-shell zeolitic imidazolate framework-8 (ZIF-8)@Fe