A5026737180- Magnetism in coordination complexes
- Organic and Molecular Conductors Research
- X-ray Diffraction in Crystallography
- Electrochemical Analysis and Applications
- Crystallization and Solubility Studies
- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Metal complexes synthesis and properties
- Antimicrobial Peptides and Activities
- Metalloenzymes and iron-sulfur proteins
- Supramolecular Self-Assembly in Materials
- Electrochemical sensors and biosensors
- Metal-Organic Frameworks: Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Crystallography and molecular interactions
- Metal-Catalyzed Oxygenation Mechanisms
- Lanthanide and Transition Metal Complexes
- Pharmacogenetics and Drug Metabolism
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Molecular Junctions and Nanostructures
- Crystal structures of chemical compounds
- Force Microscopy Techniques and Applications
- Cholinesterase and Neurodegenerative Diseases
- Biochemical and Structural Characterization
Monash University
2016-2025
Australian Regenerative Medicine Institute
2007-2024
Département de Chimie Moléculaire
2021
Clayton Foundation
2015-2018
ARC Centre of Excellence for Electromaterials Science
2017
Queensland University of Technology
2014
Centro Brasileiro de Pesquisas Físicas
2013
University of Oxford
2012
Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati
2012
European Organization for Nuclear Research
2012
Small proline-rich antimicrobial peptides (AMP) have attracted considerable interest, as they target specific intracellular bacterial components and do not act by lytic mechanisms. Here, a novel peptide, termed oncocin (VDKPPYLPRPRPPRRIYNR-NH2), is reported that was optimized for the treatment of Gram-negative pathogens. Its minimal inhibitory concentrations in tryptic soy broth medium ranged from 0.125 to 8 μg/mL 34 different strains clinical isolates Enterobacteriaceae nonfermenters, such...
ZnO surface properties control cytotoxicity by regulating nanoparticle uptake rather than altering either intracellular or extracellular Zn dissolution rates.
Bacterial resistance is among the most serious threats to human health globally, and many bacterial isolates have emerged that are resistant all antibiotics in monotherapy. Aminoglycosides often used combination therapies against severe infections by multidrug-resistant bacteria. However, models quantifying different antibacterial effects of aminoglycosides lacking. While mode aminoglycoside action on protein synthesis has been studied, their disruptive outer membrane Gram-negative bacteria...
Drug-resistant bacteria pose an urgent global health threat, necessitating the development of antibacterial compounds with novel modes action. Protein biosynthesis accounts for up to half energy expenditure bacterial cells, and consequently inhibiting efficiency or fidelity ribosome is a major target existing antibiotics. Here, we describe alternative mode action that affects same process: allowing translation proceed but causing co-translational aggregation nascent peptidic chain. We show...
Rhus vernicifera (Rv) laccase was purified to electrophoretic homogeneity by hydrophobic interaction chromatography. A comprehensive study of the direct electrochemistry Rv covalently immobilized at a gold electrode using alkanethiol monolayers undertaken. The observed midpoint potential 410 mV versus normal hydrogen (NHE), consistent with reduction potentials obtained potentiometric titration for T1 copper site. Evidence is presented concerted 4-electron reversible process slow scan rates...
The emergence of multiple-drug-resistant (MDR) bacterial pathogens in hospitals (nosocomial infections) presents a global threat growing importance, especially for Gram-negative bacteria with extended spectrum β-lactamase (ESBL) or the novel New Delhi metallo-β-lactamase 1 (NDM-1) resistance. Starting from antibacterial peptide apidaecin 1b, we have optimized sequence to treat systemic infections most threatening human pathogens, such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas...
Amyloid protein (Aβ1–40) aggregation and conformation was examined using native sodium dodecyl sulfate/polyacrylamide gel electrophoresis, the results compared with those obtained by atomic force microscopy, Congo red binding, sedimentation turbidity assays. The amount of Aβ measured different, depending upon method used. Incubation for 15 min at pH 5.0 or in presence Fe 2+ , Cu Zn did not alter level oligomers observed on SDS gels. However, slow to form high molecular mass species over 5...
A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology allows application this to long-term studies including direct electrochemistry.
Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints avoiding degenerative diseases such as osteoarthritis. Immobilized surface is thin, interfacial layer macromolecules consisting mainly hyaluronic acid (HA) lubricin (LUB; a.k.a. PRG4) that believed to form co-adsorbed, composite film now known exhibit synergistic tribological properties. Bioinspired by composition surfaces, layers HA LUB were grafted Au...
The [3Fe-4S]+/0 cluster of Azotobacter vinelandii ferredoxin I (AvFdI) has an unusually low and strongly pH-dependent reduction potential (E'0). reduced exists in two forms, depending upon pH, that exhibit substantially different magnetic circular dichroism (MCD) spectra. Recent studies have established the MCD changes observed on decreasing pH from 8.3 (alkaline form) to 6.0 (acid cannot be explained either by a change spin state (Stephens, P.J., Jensen, G.M., Devlin, F.J., Morgan, T.V.,...
The beta-amyloid protein (Abeta) is the major component of amyloid plaques found in Alzheimer brain. Although there a loss acetylcholinesterase (AChE) from both cholinergic and non-cholinergic neurones brain patients, level AChE increased around plaques. Previous studies using P19 cells culture transgenic mice which overexpress human Abeta have suggested that this increase may be due to direct action on expression adjacent aim present study was examine mechanism by increases levels primary...
Abstract The deposition of transthyretin (TTR) amyloid in the PNS is a major pathological feature familial amyloidotic polyneuropathy. aim present study was to examine whether TTR could disrupt cytoplasmic Ca 2+ homeostasis and determine role aggregation this process. amyloidogenic examined by solution turbidity, dynamic light scattering atomic force microscopy. A nucleation‐dependent polymerization process observed which formed low molecular weight aggregates (oligomers < 100 nm...
The in situ reduction of dissolved Ag+ or Au3+ to the zero valent state distillable ionic liquid DIMCARB is reported. process leads both 1D and 3D nanostructure formation, i.e. nanoparticles nanowires clusters composed nanoparticles. nanostructures formed have been characterised using UV/vis spectroscopy, powder X-ray diffraction atomic force microscopic (AFM) topographic phase imaging. Ag growth occurs as a solution based crystallisation process. However, real time AFM imaging when...
Abstract The amphibian skin is a vast resource for bioactive peptides, which form the basis of animals' innate immune system. Key components secretions cutaneous glands are antimicrobial peptides (AMPs), exert their cytotoxic effects often as result membrane disruption. It becoming increasingly evident that there link between mechanism action AMPs and amyloidogenic proteins. In this work, we demonstrate broad‐spectrum AMP uperin 3.5, has random‐coil structure in solution but adopts an...