A5066070723- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Membrane-based Ion Separation Techniques
- Eosinophilic Disorders and Syndromes
- Fuel Cells and Related Materials
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- Protein Structure and Dynamics
- Cell death mechanisms and regulation
- Veterinary medicine and infectious diseases
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Analog and Mixed-Signal Circuit Design
- Viral Infections and Immunology Research
- Endoplasmic Reticulum Stress and Disease
- Hippo pathway signaling and YAP/TAZ
- Machine Learning in Bioinformatics
- Phagocytosis and Immune Regulation
- Acoustic Wave Resonator Technologies
- Genetics, Bioinformatics, and Biomedical Research
- Synthesis of β-Lactam Compounds
- Cardiac electrophysiology and arrhythmias
The University of Melbourne
2019-2023
Walter and Eliza Hall Institute of Medical Research
2018-2023
Abstract Almost all interactions and reactions that occur in living organisms involve proteins. The various biological roles of proteins include, but are not limited to, signal transduction, gene transcription, cell death, immune function, structural support, catalysis the chemical enable to survive. varied have led them being dubbed ‘the workhorses organisms’. This article discusses functions how protein function is studied a laboratory setting. In this article, we begin by examining...
Potent neutralizing monoclonal antibodies are one of the few agents currently available to treat COVID-19. SARS-CoV-2 variants concern (VOCs) that carry multiple mutations in viral spike protein can exhibit neutralization resistance, potentially affecting effectiveness some antibody-based therapeutics. Here, generation a diverse panel 91 human, provides an in-depth structural and phenotypic definition receptor binding domain (RBD) antigenic sites on spike. These RBD ameliorate infection mice...
Abstract The necroptosis pathway is a lytic, pro-inflammatory mode of cell death that widely implicated in human disease, including renal, pulmonary, gut and skin inflammatory pathologies. precise mechanism the terminal steps pathway, where RIPK3 kinase phosphorylates triggers conformation change oligomerization effector, MLKL, are only emerging. Here, we structurally identify RIPK3-mediated phosphorylation MLKL activation loop as cue for pseudokinase domain dimerization. dimerization...
Abstract Ion currents through potassium channels are gated. Constriction of the ion conduction pathway at inner helix bundle, textbook gate Kir channels, has been shown to be an ineffective permeation control, creating a rift in our understanding how these Here we present evidence that anionic lipids act as interactive response elements sufficient conduction. We demonstrate limiting barrier K + lies within and show this is operated by fatty acyl tails infiltrate via fenestrations walls pore....
Abstract The canonical mechanistic model explaining potassium channel gating is of a conformational change that alternately dilates and constricts collar-like intracellular entrance to the pore. It based on premise K + ions maintain complete hydration shell while passing between transmembrane cavity cytosol, which must be accommodated. To put test, we locked conformation Kir prevent widening narrow collar. Unexpectedly, conduction was unimpaired in channels. In parallel, employed all-atom...
Abstract Human 12-lipoxygenase (12-LOX) is an enzyme involved in platelet activation and a promising target for antiplatelet therapies. Despite the clinical importance of 12-LOX, exact mechanisms how it affects are unclear, lack structural information has limited drug discovery efforts. In this study, we used single-particle cryoelectron microscopy to determine high-resolution structures (1.7 Å - 2.8 Å) human 12-LOX first time. Our results showed that can exist multiple oligomeric states,...
Abstract Ion currents through potassium channels are gated. Constriction of the ion conduction pathway at inner helix bundle, textbook ‘gate’ Kir channels, has been shown to be an ineffective permeation control, creating a rift in our understanding how these Here we present first evidence that anionic lipids act as interactive response elements sufficient gate conduction. We demonstrate limiting barrier K + lies within and show this is operated by fatty acyl tails infiltrate via...