A5023032968- Toxoplasma gondii Research Studies
- Amoebic Infections and Treatments
- Cellular transport and secretion
- Signaling Pathways in Disease
- HIV Research and Treatment
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Parasitic Infections and Diagnostics
- Pancreatitis Pathology and Treatment
- HIV/AIDS Research and Interventions
- Cellular Mechanics and Interactions
- Cytomegalovirus and herpesvirus research
- Invertebrate Immune Response Mechanisms
- Tuberculosis Research and Epidemiology
- Biliary and Gastrointestinal Fistulas
- Lipid Membrane Structure and Behavior
- Pediatric Hepatobiliary Diseases and Treatments
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Malaria Research and Control
- Biochemical and Molecular Research
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Herpesvirus Infections and Treatments
- Superconductivity in MgB2 and Alloys
Centre National de la Recherche Scientifique
2012-2023
Inserm
2012-2023
Center for Infection and Immunity of Lille
2017-2023
Institut Pasteur de Lille
2017-2023
Centre Hospitalier Universitaire de Lille
2017-2023
Université de Lille
2017-2022
Institut Pasteur
2000-2020
Institut Cochin
2011-2019
Délégation Paris 5
2012-2016
Sorbonne Paris Cité
2012-2016
The Diaphanous-related formin Dia1 nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of to spatially define polymerization are largely unknown. In this study, we identify cytoskeletal scaffold protein IQGAP1 as a Dia1-binding is necessary for its subcellular location. interacts with through region within Diaphanous inhibitory domain after RhoA-mediated release autoinhibition. Both proteins colocalize at front migrating...
Phagocytosis of human cells is a crucial activity for the virulence parasite Entamoeba histolytica. This protozoan invades and destroys intestine by killing phagocytosing epithelial cells, erythrocytes from immune system. In this study, we used magnetic beads covered with proteins serum as model system to study early events involved in phagocytosis E. We validated showing that uptake triggered activation actin-myosin cytoskeleton PI3-kinase previously described erythrophagocytosis. purified...
Mycobacterium tuberculosis (Mtb) is a successful intracellular pathogen that thrives in macrophages (Mφs). There need to better understand how Mtb hijacks cellular processes like phagolysosome biogenesis, classical pathogenesis determinant. A central feature this microbial strategy the manipulation of actin Mφs. Here, we examine role microRNAs (miRNAs) as potential mechanism regulation actin-mediated events leading phagocytosis context mycobacteria infection. Given non-virulent smegmatis...
Phagosome maturation is defined as the process by which phagosomes fuse sequentially with endosomes and lysosomes to acquire an acidic pH hydrolases that degrade ingested particles. While essential role of actin cytoskeleton remodeling during particle internalization well established, its later stages phagosome remains largely unknown. We have previously shown purified mature assemble F‐actin at their membrane, ezrin‐radixin‐moesin (ERM) proteins ezrin moesin participate in this process....
Toxoplasma gondii possesses a highly polarized secretory system, which efficiently assembles de novo micronemes and rhoptries during parasite replication. These apical organelles release their contents into host cells promoting invasion survival. Using CreLox-based inducible knock-out strategy the ddFKBP over-expression we unraveled novel functions of clathrin adaptor complex TgAP1. First, our data indicate that AP1 in T. likely as conserved heterotetrameric composed four subunits γ, β, μ1,...
Toxoplasma gondii possesses an armada of secreted virulent factors that enable parasite invasion and survival into host cells. These are contained in specific secretory organelles, the rhoptries, micronemes dense granules release their content upon cell recognition. Dense a constitutive manner during replication play crucial role modulating metabolic immune responses. While molecular mechanisms triggering rhoptry microneme adhesion have been well studied, secretion remains poorly explored...
The human parasite Entamoeba histolytica is an ancient protozoan that expresses only one unconventional myosin, which has homology with myosin IB from other amoebae. Myosin involved in phagocytosis of cells by E. histolytica. In this work, we developed a microrheological technique, analysing magnetic phagosomes, allowed us to probe the density F-actin network living cells. Using showed overexpression led increase cytoplasm viscosity, correlated delay initiating cell phagocytosis. To...
Phospholipid Scramblase 1 (PLSCR1) was initially characterized as a type II transmembrane protein involved in bilayer movements of phospholipids across the plasma membrane leading to cell surface exposure phosphatidylserine, but other cellular functions have been ascribed this signaling processes and nucleus. In present study, expression PLSCR1 were explored specialized phagocytic cells monocyte/macrophage lineage. The found be markedly increased monocyte-derived macrophages compared...
Abstract Although a number of infants with maternal uniparental disomy chromosome 16 (upd(16)mat) have been reported, the evidence for imprinting on is not yet conclusive. To test hypothesis that upd(16)mat has distinct phenotype, which would support existence imprinted gene(s) 16, statistical analysis was performed large series (n = 83) mosaic trisomy cases molecular determination status. The incidence 40%, consistent expected one third from random loss during rescue ( P 0.262). In pairwise...
Dendritic cells are the most powerful antigen-presenting of immune system. They present exogenous antigens associated with Major Histocompatibility Complex (MHC) Class II molecules through classical pathway to stimulate CD4+ T cells, or MHC-I activate CD8+ lymphocytes cross-presentation pathway. DCs represent one main cellular targets during infection by Toxoplasma gondii. This intracellular parasite incorporates essential nutrients, such as cholesterol, grow and proliferate inside a highly...
Members of the Apicomplexa phylum possess specialized secretory organelles that discharge, apically and in a timely regulated manner, key factors implicated parasite motility, host cell invasion, egress subversion cellular functions. The mechanisms regulating trafficking apical docking these are only partially elucidated. Here, we characterized two conserved endosomal regulators known to promote vesicle transport and/or fusion, HOOK Fused Toes (FTS), context organelle discharge Toxoplasma...
The glycosylphosphatidylinositol (GPI) moiety is one of the ways by which many cell surface proteins, such as Gal/GalNAc lectin and proteophosphoglycans (PPGs) attach to Entamoeba histolytica, agent human amoebiasis. It believed that these GPI-anchored molecules are involved in parasite adhesion cells, mucus extracellular matrix. We identified an E. histolytica homolog PIG-M, a mannosyltransferase required for synthesis GPI. sequence structural analysis led conclusion EhPIG-M1 composed...
ABSTRACT The intracellular events underlying phagocytosis, a crucial event for innate immunity, are still unresolved. In order to test whether the reservoir of membrane required formation phagocytic pseudopodia is maintained by cortical ezrin, and that its cleavage key step in releasing this membrane, ezrin was monitored within living phagocytes (the phagocytically competent cell line RAW264.7) through expressing two constructs with fluorescent protein tags located either inside FERM or at...