A5040366709- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Phagocytosis and Immune Regulation
- Cellular transport and secretion
- Calpain Protease Function and Regulation
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Caveolin-1 and cellular processes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- bioluminescence and chemiluminescence research
- Vascular Malformations Diagnosis and Treatment
Cardiff University
2018-2020
ABSTRACT The intracellular events underlying phagocytosis, a crucial event for innate immunity, are still unresolved. In order to test whether the reservoir of membrane required formation phagocytic pseudopodia is maintained by cortical ezrin, and that its cleavage key step in releasing this membrane, ezrin was monitored within living phagocytes (the phagocytically competent cell line RAW264.7) through expressing two constructs with fluorescent protein tags located either inside FERM or at...
Ezrin links the cortical cytoskeleton to plasma membrane and plays a role in regulating changes cell shape. Recently, NSC668394 has been shown inhibit key step for its activity, i.e. phosphorylation at threonine 567. In neutrophils, another regulatory is Ca2+-mediated cleavage of ezrin by calpain.In this paper, we use as pharmacological inhibitor investigate interplay between these two steps neutrophil shape.NSC668394 reduced amount peripherally located increased Ca2+-dependent cleavage....