A5037707278- Immunotherapy and Immune Responses
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Toxoplasma gondii Research Studies
- Complex Systems and Time Series Analysis
- T-cell and B-cell Immunology
- Slime Mold and Myxomycetes Research
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Neuroscience and Neuropharmacology Research
- Cancer Immunotherapy and Biomarkers
- Connexins and lens biology
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Nicotinic Acetylcholine Receptors Study
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Reproductive tract infections research
- Advanced Drug Delivery Systems
Instituto de Histología y Embriología de Mendoza
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
Universidad Nacional de Cuyo
2016-2025
Centre for Genomic Regulation
2025
Inserm
2009-2014
Institut Curie
2009-2014
National Institute of Industrial Technology
2012
National Agricultural Technology Institute
2012
Immunité et Cancer
2009-2011
National University of Misiones
2006
Abstract Gap junctions formed by the major neuronal connexin Cx36 function as electrical synapses in nervous system and provide unique functions such synchronizing neuron activities or supporting network oscillations. Although physiological significance of for networks is well established, little known about pathways that regulate transport its main component: Cx36. Here we have used HEK293T cells an expression combination with siRNA BioID screens to study transition from ER cis Golgi. Our...
Rab GTPases define the identity and destiny of vesicles. Some these small present isoforms that are expressed differentially along developmental stages or in a tissue-specific manner, hence comparative analysis is difficult to achieve. Here, we describe intracellular distribution function lipid transport poorly characterized Rab39 using typical cell biology experimental tools new ones developed our laboratory. We show that, despite their amino acid sequence similarity, Rab39a Rab39b display...
During cross-presentation, exogenous antigens (i.e. intracellular pathogens or tumor cells) are internalized and processed within the endocytic system also by proteasome in cytosol. Then, antigenic peptides associated with Major Histocompatibility Complex (MHC) class I molecules these complexes transit to plasma membrane order trigger cytotoxic immune responses through activation of CD8+ T lymphocytes. Dendritic cells (DCs) particularly adapted achieve efficient antigen cross-presentation...
Dendritic cells are the most powerful antigen-presenting of immune system. They present exogenous antigens associated with Major Histocompatibility Complex (MHC) Class II molecules through classical pathway to stimulate CD4+ T cells, or MHC-I activate CD8+ lymphocytes cross-presentation pathway. DCs represent one main cellular targets during infection by Toxoplasma gondii. This intracellular parasite incorporates essential nutrients, such as cholesterol, grow and proliferate inside a highly...
The intracellular Toxoplasma gondii parasite replicates within a parasitophorous vacuole (PV). T. secretes proteins that remain soluble in the PV space, are inserted into membranes or exported beyond boundary. In addition to supporting growth, these can be processed and presented by MHC I for CD8 + T‐cell recognition. Yet it is unclear whether membrane binding influences processing pathways employed if topology of antigens impacts their presentation. Here we report membrane‐bound differ...
Abstract Gap junctions formed by the major neuronal connexin Cx36 function as electrical synapses in nervous system and provide unique functions such synchronizing activities or network oscillations. Although physiological significance of for networks is well established, little known about pathways that regulate transport its main component: Cx36. Here we have used HEK293T cells an expression combination with siRNA BioID screens to study transition from ER cis Golgi. Our data indicate...
The recruitment of endoplasmic reticulum (ER) components to dendritic cell (DC) phagosomes and endosomes is a crucial event achieve efficient cross-presentation exogenous antigens. We have previously identified the small GTPase Rab22a as key regulator MHC-I trafficking antigen by DCs. In this study we show that low expression does not prevent normal delivery ER-derived proteins DC phagosomes. contrast, presence these was diminished in labelled with fluid phase marker. These observations were...
Reproducing cell processes using an in silico system is essential tool for understanding the underlying mechanisms and emergent properties of this extraordinary complex biological machine. However, computational models are seldom applied field intracellular trafficking. In a cell, numerous molecular interactions occur on surface or interior membrane-bound compartments that continually change position undergo dynamic fusion fission. At present, available simulation tools not suitable to...
Abstract Although the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infects lepidopteran invertebrates as natural hosts, represents an efficient vector for vaccine development. Baculovirus surface display induces strong humoral responses against viruses and parasites. A novel strategy based on capsid carrying foreign antigens in AcMNPV particle further improved immune response by eliciting CD8 + T cell activation. In this study, we analyze intracellular...
ABSTRACT Antigen cross‐presentation is the process whereby small peptides derived from exogenous antigens are attached to MHC‐I molecules triggering CD8+ T lymphocyte activation. The endocytic route of dendritic cells (DCs) highly specialised for initiate cytotoxic immune responses against numerous intracellular pathogens and tumours. In this study, we identify endosomal protein sorting nexin (SNX) 17 as a key regulator antigen internalisation by DCs. SNX17 expression in DCs guarantees...
A live system to release heterologous antigens using an attenuated Salmonella strain was developed. We transformed typhimurium LVR03 (S. LVR03) with a recombinant pTECH2 vector encoding 0, 1, 2, and 4 tandem copies of imunogenic peptide bovine herpes virus-1 (BoHV-1) glycoprotein D (gD). The used yielded peptides fused the non-toxic C fragment tetanus toxin (TetC), which has been shown have adjuvant properties. Inoculation BALB/c mice strains gave rise mild self-limited infection, primary...
Abstract The immunoregulatory cation channel TMEM176B plays a dual role in tumor immunity. On one hand, promotes antigen cross-presentation to CD8 + T cells by regulating phagosomal pH dendritic (DCs). the other inhibits NLRP3 inflammasome activation through ionic mechanisms DCs, monocytes and macrophages. Moreover, blocker BayK8644 controls progression involving prophylactic but not therapeutic protocols. We speculated that limited efficacy of compound may be linked its potential capacity...
The immunoregulatory cation channel TMEM176B expressed in myeloid cells plays a dual role tumor immunity. On one hand, promotes antigen cross-presentation to CD8+ T by regulating phagosomal pH dendritic (DCs), leading activation of adaptive the other inhibits NLRP3 inflammasome through ionic mechanisms DCs, monocytes and macrophages, thus inhibiting innate Moreover, blocker BayK8644 controls progression involving prophylactic but not therapeutic protocols. We speculated that limited efficacy...