A5091628067- Connexins and lens biology
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Zebrafish Biomedical Research Applications
- Nicotinic Acetylcholine Receptors Study
- Cephalopods and Marine Biology
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Chemical synthesis and alkaloids
- Genetics, Aging, and Longevity in Model Organisms
- Retinal Development and Disorders
- Plant responses to elevated CO2
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Ophthalmology and Visual Impairment Studies
- Plant Water Relations and Carbon Dynamics
- Cardiomyopathy and Myosin Studies
- Advanced biosensing and bioanalysis techniques
- Spaceflight effects on biology
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- Cardiovascular Effects of Exercise
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Immune cells in cancer
University of Oregon
2015-2025
University of Wisconsin–Madison
2017-2018
Fred Hutch Cancer Center
2013-2017
Cancer Research Center
2017
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2015-2017
Washington University in St. Louis
2017
Lorain County Community College
2013
Iowa State University
1997-2002
Cochlear hair cells (HCs) are mechanosensory receptors that transduce sound into electrical signals. HC damage in nonmammalian vertebrates induces surrounding supporting (SCs) to divide, transdifferentiate and replace lost HCs; however, such spontaneous regeneration does not occur the mammalian cochlea. Here, we acutely ablate retinoblastoma protein (Rb), a crucial cell cycle regulator, two subtypes of postmitotic SCs (pillar Deiters9 cells) using an inducible Cre line,...
Forward genetic screens have elucidated molecular pathways required for innumerable aspects of life; however, identifying the causal mutations from such has long been bottleneck in process, particularly vertebrates. We developed an RNA-seq–based approach that identifies both region genome linked to a mutation and candidate lesions may be phenotype interest. show our method successfully zebrafish cause nonsense or missense changes codons, alter transcript splicing, gene expression levels....
Cephalopods have a remarkable visual system, with camera-type eye and high acuity vision that they use for wide range of sophisticated visually driven behaviors. However, the cephalopod brain is organized dramatically differently from vertebrates invertebrates, beyond neuroanatomical descriptions, little known regarding cell types molecular determinants their system organization. Here, we present comprehensive single-cell atlas octopus optic lobe, which primary processing structure in brain....
For many plants growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role that leaf ontogeny plays acclimation response, we monitored photosynthesis and some related parameters at short intervals throughout ontogenetic development tobacco (Nicotiana tabacum L.) leaves under ambient (350 [mu]L L-1)- high (950 L-1)-CO2 conditions. The pattern photosynthetic rate over time was similar between two treatments consistent with expected for a typical dicot leaf. However,...
The nematode Caenorhabditis elegans migrates toward a preferred temperature on thermal gradient. A candidate neural network for thermotaxis in C. has been identified, but the behavioral strategy implemented by this is poorly understood. In study, we tested whether migration achieved modulating probability of turning behavior, as chemotaxis. This was done subjecting unrestrained wild-type, cryophilic, or thermophilic worms to rapid spatially uniform steps (3 degrees C), up down from...
Neural network function is based upon the patterns and types of connections made between neurons. Neuronal synapses are adhesions specialized for communication they come in two types, chemical electrical. Communication at occurs via neurotransmitter release whereas electrical utilize gap junctions direct ionic metabolic coupling. Electrical often viewed as symmetrical structures, with same components making both sides junction. By contrast, we show that a broad set zebrafish, Danio rerio,...
Electrical synaptic transmission relies on neuronal gap junctions containing channels constructed by Connexins. While at chemical synapses neurotransmitter-gated ion are critically supported scaffolding proteins, it is unknown if electrical require similar scaffold support. Here, we investigated the functional relationship between Connexins and Zonula Occludens 1 (ZO1), an intracellular protein localized to synapses. Using model in zebrafish Mauthner cells, demonstrated that ZO1 required for...
In this review article, we will describe the recent advances made towards understanding molecular and cell biological mechanisms of electrical synapse formation. New evidence indicates that synapses, which are gap junctions between neurons, can have complex compositions including protein asymmetries across joined cells, diverse morphological arrangements, overlooked similarities with other junctions, all indicate new potential roles in neurodevelopmental disease. Aquatic organisms,...
The zebrafish (
Abstract Myopia is the most common developmental disorder of juvenile eyes, and it has become an increasing cause severe visual impairment. The GJD2 locus been consistently associated with myopia in multiple independent genome-wide association studies. However, despite strong genetic evidence, little known about functional role refractive error development. Here, we find that depletion gjd2a (Cx35.5) or gjd2b (Cx35.1) orthologs zebrafish, changes biometry status eye. Our immunohistological...
Summary Octopuses, squids, and cuttlefishes – the coleoid cephalopods are a remarkable branch in tree of life whose members exhibit repertoire sophisticated behaviors (Hanlon Messenger, 2018). As clade, coleoids harbor an incredible variety novel traits including most complex nervous system among invertebrates, derived camera-type eyes, rapid adaptive camouflage abilities (Young, 1971; Hanlon, 2007). The burst evolutionary novelty that distinguishes is even more striking phylogenetic...
Abstract Gap junctions formed by the major neuronal connexin Cx36 function as electrical synapses in nervous system and provide unique functions such synchronizing neuron activities or supporting network oscillations. Although physiological significance of for networks is well established, little known about pathways that regulate transport its main component: Cx36. Here we have used HEK293T cells an expression combination with siRNA BioID screens to study transition from ER cis Golgi. Our...
The nematode Caenorhabditis elegans migrates toward a preferred temperature on thermal gradient. A candidate neural network for thermotaxis in C. has been identified, but the behavioral strategy implemented by this is poorly understood. In study, we tested whether migration achieved modulating probability of turning behavior, as chemotaxis. This was done subjecting unrestrained wild-type, cryophilic, or thermophilic worms to rapid spatially uniform steps (3°C), up down from cultivation...