A5060270544- Corneal surgery and disorders
- Ophthalmology and Visual Impairment Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Connexins and lens biology
- Glaucoma and retinal disorders
- Parkinson's Disease Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Microbial infections and disease research
- Retinal Diseases and Treatments
- Amyotrophic Lateral Sclerosis Research
- Molecular Biology Techniques and Applications
- Prion Diseases and Protein Misfolding
- Cholinesterase and Neurodegenerative Diseases
- Cancer Genomics and Diagnostics
- Retinal and Macular Surgery
- Cancer-related molecular mechanisms research
- Ocular and Laser Science Research
- Cervical Cancer and HPV Research
- Redox biology and oxidative stress
Erasmus MC
2021-2023
AXON Neuroscience (Slovakia)
2020
University of Applied Sciences Leiden
2014
Abstract Myopia is the most common developmental disorder of juvenile eyes, and it has become an increasing cause severe visual impairment. The GJD2 locus been consistently associated with myopia in multiple independent genome-wide association studies. However, despite strong genetic evidence, little known about functional role refractive error development. Here, we find that depletion gjd2a (Cx35.5) or gjd2b (Cx35.1) orthologs zebrafish, changes biometry status eye. Our immunohistological...
Myopia, or nearsightedness, is the most common type of refractive error and characterized by a mismatch between optical power ocular axial length. Light, more specifically spectral composition light, has been known to influence myopic growth. In this pilot study, we exposed zebrafish illuminations that vary in screened for changes The illumination spectra included narrow band ultra-violet A (UVA) (peak wavelength 369 nm), violet (425 cyan (483 green/yellow (557 red (633 nm) as well broad...
Purpose: To establish a set of assays that allow the in vivo screening candidate genes for ocular diseases zebrafish, with an emphasis on refractive error. Methods: Our pipeline includes most relevant measurements to assess (1) biometry using spectral domain optical coherence tomography, (2) status eccentric photorefractor, (3) intraocular pressure by tonometry, and (4) optokinetic response study visual capability zebrafish. validate our demonstrate potential zebrafish as valid animal model,...
Summary Changes in the genome wide DNA methylation landscape are hallmarks of cancer cells and precursor lesions cancers. To capitalize on utilizing for detection classification cancer, we generated a database gynecological cancers associated healthy tissues using Methylated sequencing (MeD-seq). We show that target cell enrichment to generate is crucial marker discovery report range novel biomarkers tissue origin determination developed subset these biomarkers, both intragenic intergenic,...
Neuronal accumulation of hyperphosphorylated and truncated tau aggregates is one the major defining factors key drivers neurodegeneration in Alzheimer's disease other tauopathies.We developed an AAV-induced model tauopathy mediated by human protein without familial frontotemporal dementia-related mutations to study propagation functional consequences pathology.We performed targeted transductions hippocampus or entorhinal cortex adult mice followed histological analysis progression...
The hexanucleotide (G4C2)-repeat expansion in the C9ORF72 gene is most common pathogenic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). This repeat can be translated into dipeptide proteins (DPRs), distribution poly-GR DPR correlates with neurodegeneration postmortem C9FTD/ALS brains. Here, we assessed toxicity zebrafish embryos, using an annexin A5-based fluorescent transgenic line (secA5) that allows for detection quantification apoptosis vivo....
Abstract Genome-wide association studies (GWAS) have dissected numerous genetic factors underlying refractive errors (RE) such as myopia. Despite significant insights into understanding the architecture of RE, few validated and explored functional role candidate genes within these loci. To functionally follow-up on GWAS characterize potential development we prioritized nine ( TJP2 , PDE11A SHISA6 LAMA2 LRRC4C KCNQ5 GNB3 RBFOX1 GRIA4 ) based biological statistical evidence; used CRISPR/cas9...
Alzheimer's disease (AD) and other tauopathies are neurodegenerative disorders characterized by cellular accumulation of aggregated tau protein. Tau pathology within these is accompanied chronic neuroinflammation, such as activation the classical complement pathway initiation factor C1q. Additionally, about half AD cases present with inclusions composed alpha-synuclein called Lewy bodies. bodies in Parkinson's body dementia also frequently occur together pathology.Immunotherapy currently...
Abstract Background Alzheimer’s disease (AD) and other tauopathies are neurodegenerative disorders characterized by cellular accumulation of aggregated tau protein. Tau pathology within these is accompanied chronic neuroinflammation, such as activation the classical complement pathway initiation factor C1q. Additionally, about half AD cases present with inclusions composed alpha-synuclein called Lewy bodies. bodies in Parkinson’s body dementia also frequently occur together pathology....