A5051812492- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Insect Resistance and Genetics
- Venomous Animal Envenomation and Studies
- COVID-19 Clinical Research Studies
- Invertebrate Immune Response Mechanisms
- vaccines and immunoinformatics approaches
- Healthcare and Venom Research
- Veterinary Equine Medical Research
- Hormonal and reproductive studies
- Vaccine Coverage and Hesitancy
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Social Issues and Policies in Latin America
- Public Health and Environmental Issues
Universidad Nacional de Córdoba
2013-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2009-2025
Research Centre in Biological Chemistry of Córdoba
2018-2023
National University of the Northeast
2020
Cordoba University
2016
Ghent University
2011
Institut Pasteur
2002-2008
Inserm
2003-2008
Abstract Background The WHO states that antivenom is the only safe and effective treatment to neutralize snake venom. Snakebite typically involves horse hyperimmunization with crude venom Freund's adjuvant. Methods In current work, we analyzed ascorbyl palmitate liquid crystal structure protein or PLA2, carrier charge capacity, evaluated immune response induced by enzyme P9a(Cdt-PLA2) formulated in a nanostructure using CpG-ODN, determining titer of IgG antibodies. BALB/c mice were...
Recombinant porcine parvovirus virus-like particles (PPV-VLPs) are particulate exogenous antigens that induce a strong, specific cytotoxic T lymphocyte (CTL) response in the absence of adjuvant. In present report, we demonstrate vivo dendritic cells (DCs) PPV-VLPs to CD8+ after intracellular processing. captured by DCs with high efficacy, which results delivery these 50% whole spleen DC population. vivo, few hours injection, presented exclusively CD8α− DCs, whereas 15 later they mainly CD8α+...
Although much is described about the molecules involved in neutrophil migration from circulation into tissues, less known molecular mechanisms that regulate entry lymph nodes (LNs) draining a local inflammatory site. In this study, we investigated toward LNs context of inflammation induced by immunization BALB/c mice with OVA emulsified CFA. We demonstrated neutrophils can enter OVA/CFA-immunized not only via lymphatic vessels but also blood, across high endothelial venules. By adoptive...
The aging process is accompanied by altered immune system functioning and an increased risk of infection. Dendritic cells (DCs) are antigen-presenting that play a key role in both adaptive innate immunity, but how affects DCs their influence on immunity has not been thoroughly established. Here we examined the function conventional (cDCs) old mice after TLR7 stimulation, focusing ability to cross-prime CD8+ T cells. Using polyU, synthetic ssRNA analog, as ligand OVA antigen (Ag) model, found...
Abstract We have previously demonstrated that subcutaneously administered ovalbumin (OVA) plus synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs (CpG-ODN) as adjuvant stimulate cellular and humoral immunity promote T helper cell type 1 differentiation in aged mice. The present study assessed the ability of CpG-ODN to induce an OVA-specific immune response after oral immunization young (3-month-old) (18-month-old) BALB/c Oral OVA/CpG-ODN induces a similar...
Toll-like receptor (TLR) ligands may be a valuable tool to promote antitumor responses by reinforcing immunity. In addition their expression in immune cells, functional TLRs are also expressed many cancer but significance has been controversial. this study, we examined the action of TLR on tumor pathophysiology as result direct cell effects. B16 murine melanoma cells were stimulated vitro with TLR4 ligand (LPS-B16) prior inoculation into TLR4-deficient mice (Tlr4 (lps-del)). Under such...
Abstract ssRNA can interact with dendritic cells (DCs) through binding to TLR7, inducing secretion of proinflammatory cytokines and type I IFN. Triggering TLR7 enhances cross-priming CD8+ T cells, which requires cross-presentation exogenous Ag DCs. However, how TLR triggering affect is still not clear. Using OVA as an model, we observed that stimulation in DCs by polyuridylic acid (polyU), a synthetic analog, generates strong specific cytotoxic response C57BL/6 mice. PolyU stimulate CD8α+...
Baculoviruses (BV) are DNA viruses that pathogenic for insects. Although BV infect a range of mammalian cell types, they do not replicate in these cells. Indeed, the potential effects insect on immune responses mammals only just beginning to be studied. We show this paper recombinant Autographa californica multiple nuclear polyhedrosis virus carrying fragment ovalbumin (OVA) VP39 capsid protein (BV-OVA) has capacity act as an adjuvant and vector antigens mice, thereby promoting specific CD4...
It is well known that neutrophils are rapidly recruited to a site of injury or infection and perform critical role in pathogen clearance inflammation. However, they also able interact with regulate innate adaptive immune cells some stimuli induce the migration lymph nodes (LNs). Previously, we demonstrated complex (IC) generated by injecting OVA into footpad OVA/CFA immunized mice induced OVA+ draining LNs (dLNs). Here investigate effects these which reach dLNs on CD4+ T cell response. Our...
Abstract The range of vaccines developed against severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) provides a unique opportunity to study immunization across different platforms. In single-center cohort, we analyzed the humoral and cellular immune compartments following five disease 2019 (COVID-19) spanning three technologies (adenoviral, mRNA inactivated virus) administered in 16 combinations. For adenoviral inactivated-virus vaccines, heterologous combinations were generally...
Recognition of microbial products by macrophages (Mphi) stimulates an inflammatory response and plays a critical role in directing the host immune against infection. In present work, we showed for first time that synthetic oligodeoxynucleotides containing unmethylated cytosine guanine motifs (CpG) are able to stimulate, presence interferon-gamma (IFN-gamma), both arginase inducible nitric oxide synthase (iNOS) murine Mphi. Unexpectedly, IFN-gamma, cytokine believed be inhibitor activity,...
There is a need for new vaccine adjuvant strategies that offer both vigorous antibody and T-cell mediated protection to combat difficult intracellular pathogens cancer. To this aim, we formulated class-B synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) with nanostructure (Coa-ASC16 or coagel) formed by self-assembly of 6-0-ascorbyl palmitate ester. Our previous results demonstrated mice immunized ovalbumin (OVA) CpG-ODN Coa-ASC16...
Abstract The crucial role that endogenously produced IFN-β plays in eliciting an immune response against cancer has recently started to be elucidated. Endogenous important surveillance and control of tumor development. Accordingly, the TLR agonists as therapeutic agents is being revisited via strategy intra/peritumoral injection with idea stimulating production endogenous type I IFN inside tumor. Polyadenylic-polyuridylic acid (poly A:U) a dsRNA mimetic explored empirically immunotherapy...
COVID-19 severity has been linked to an increased production of inflammatory mediators called "cytokine storm". Available data is mainly restricted the first international outbreak and reports highly variable results. This study compares demographic clinical features patients with from Córdoba, Argentina, during two waves pandemic analyzes association between comorbidities disease outcome storm", offering added value field. We investigated serum concentration thirteen soluble mediators,...
We have previously developed an antigen-delivery system based on hybrid recombinant porcine parvovirus-like particles (PPV-VLPs) formed by the self-assembly of VP2 protein PPV carrying a foreign epitope at its N terminus. In this study, different constructs were made containing CD8+ T-cell chicken ovalbumin (OVA) to analyse influence sequence inserted into correct processing VLPs antigen-presenting cells. analysed presentation OVA without flanking sequences or with either natural from...
// María F. Harman 1 , Romina P. Ranocchia Carolina V. Gorlino Sánchez Vallecillo Sofía D. Castell I. Crespo Belkys A. Maletto Gabriel Morón C. Pistoresi-Palencia Centro de Investigación en Bioquímica Clínica e Inmunología, Consejo Nacional Investigaciones Científicas y Técnicas, Facultad Ciencias Químicas, Universidad Córdoba, Argentina Correspondence to: Pistoresi-Palencia, e-mail: cpistore@fcq.unc.edu.ar Florencia Harman, mharman@fcq.unc.edu.ar Keywords: aging, myeloid-derived suppressor...
T cell activation requires the processing and presentation of antigenic peptides in context a major histocompatibility complex (MHC complex). Cross-dressing is non-conventional antigen mechanism, involving transfer preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to membrane professional antigen-presenting (APCs), dendritic (DCs). This an essential mechanism for induction immune response against viral antigens, tumors, graft rejection, which until now has not...