Diego Alignani
A5029344488- Multiple Myeloma Research and Treatments
- Immune cells in cancer
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Protein Degradation and Inhibitors
- Bladder and Urothelial Cancer Treatments
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Synthesis and Biological Evaluation
- Quinazolinone synthesis and applications
- S100 Proteins and Annexins
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Biosimilars and Bioanalytical Methods
- COVID-19 Clinical Research Studies
Clinica Universidad de Navarra
2016-2025
Navarre Institute of Health Research
2015-2025
Centro de Investigación Biomédica en Red de Cáncer
2019-2025
Universidad de Navarra
2014-2024
Universitat de Barcelona
2024
Hospital Clínic de Barcelona
2024
Universidade de São Paulo
2023
Clinics Hospital of Ribeirão Preto
2023
Centro de Investigación Biomédica en Red
2021
Instituto de Salud Carlos III
2020
Abstract Purpose: Myeloid-derived suppressor cells (MDSC) are considered an important T-cell immunosuppressive component in cancer-bearing hosts. The factors that attract these to the tumor microenvironment poorly understood. IL8 (CXCL8) is a potent chemotactic factor for neutrophils and monocytes. Experimental Design: MDSC were characterized sorted by multicolor flow cytometry on ficoll-gradient isolated blood leucokytes from healthy volunteers (n = 10) advanced cancer patients 28). In...
Knowledge about the mechanism of action (MoA) monoclonal antibodies (mAb) is required to understand which patients with multiple myeloma (MM) benefit most from a given mAb, alone or in combination therapy. Although there considerable research daratumumab, knowledge other anti-CD38 mAbs remains scarce.We performed comprehensive analysis MoA isatuximab.Isatuximab induces internalization CD38 but not its significant release MM cell surface. In addition, we uncovered an association between...
Background: Cardiac fibroblasts (CFs) have a central role in the ventricular remodeling process associated with different types of fibrosis. Recent studies shown that do not respond homogeneously to heart injury. Because limited set bona fide fibroblast markers, proper characterization population heterogeneity response cardiac damage is lacking. The purpose this study was define CF during and underlying mechanisms regulate function. Methods: Collagen1α1–GFP (green fluorescent...
Recent studies have found that tumor-infiltrating lymphocytes (TIL) expressing PD-1 can recognize autologous tumor cells, suggesting cells derived from PD-1+ TILs be used in adoptive T-cell therapy (ACT). However, no study thus far has evaluated the antitumor activity of PD-1-selected vivo In two mouse models solid tumors, we show allows identification and isolation tumor-specific without previous knowledge their antigen specificities. Importantly, despite high proportion tumor-reactive T...
The development of sensitive and non-invasive "liquid biopsies" presents new opportunities for longitudinal monitoring tumor dissemination clonal evolution. number circulating cells (CTCs) is prognostic in multiple myeloma (MM), but there little information on their genetic features. Here, we have analyzed the genomic landscape CTCs from 29 MM patients, including eight cases with matched/paired bone marrow (BM) cells. Our results show that 100% mutations patient BM were detected 99% present...
Identification of new markers associated with long-term efficacy in patients treated CAR T cells is a current medical need, particularly diseases such as multiple myeloma. In this study, we address the impact density on functionality BCMA cells. Functional and transcriptional studies demonstrate that high expression construct show an increased tonic signaling up-regulation exhaustion vitro cytotoxicity but decrease vivo BM infiltration. Characterization gene regulatory networks using...
Early hematopoiesis is a continuous process in which hematopoietic stem and progenitor cells (HSPCs) gradually differentiate toward specific lineages. Aging myeloid malignant transformation are characterized by changes the composition regulation of HSPCs. In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize an enriched population human HSPCs obtained from young elderly healthy individuals. Based on their transcriptional profile, identified proportions compartments...
Genome instability is related to disease development and carcinogenesis. DNA lesions are caused by genotoxic compounds but also the dysregulation of fundamental processes like transcription, replication mitosis. Recent evidence indicates that impaired expression RNA-binding proteins results in mitotic aberrations formation transcription-associated RNA-DNA hybrids (R-loops), events strongly associated with injury. We identify splicing regulator SLU7 as a key mediator genome stability....
Multiple myeloma (MM) is an incurable disease, whose clinical heterogeneity makes its management challenging, highlighting the need for biological features to guide improved therapies. Deregulation of specific long non-coding RNAs (lncRNAs) has been shown in MM, nevertheless, complete lncRNA transcriptome not yet elucidated. In this work, we identified 40,511 novel lncRNAs MM samples. accounted 82% and were more heterogeneously expressed than coding genes. A total 10,351 overexpressed 9,535...
Abstract Cellular differentiation requires extensive alterations in chromatin structure and function, which is elicited by the coordinated action of transcription factors. By contrast with factors, roles factors have not been systematically characterized. Here, we combine bulk ex vivo single-cell CRISPR screens to characterize role factor families hematopoiesis. We uncover marked lineage specificities for 142 revealing functional diversity among related (i.e. barrier-to-autointegration...
Listeria monocytogenes is a Gram-positive facultative intracellular pathogen which invades different cell types, including nonphagocytic cells, where it able to replicate and survive. The steps of the cellular infectious process have been well described consist bacterial entry, lysis endocytic vacuole, replication, spreading neighboring cells. To study listerial process, gentamicin survival assays, plaque formation, direct microscopy observations are typically used; however, there some...
Large-scale immune monitoring is becoming routinely used in clinical trials to identify determinants of treatment responsiveness, particularly immunotherapies. Flow cytometry remains one the most versatile and high throughput approaches for single-cell analysis; however, manual interpretation multidimensional data poses a challenge when attempting capture full cellular diversity provide reproducible results. We present FlowCT, semi-automated workspace empowered analyze large sets. It...
Normal cell counterparts of solid and myeloid tumors accumulate mutations years before disease onset; whether this occurs in B lymphocytes lymphoma remains uncertain. We sequenced multiple stages the lineage elderly individuals patients with lymphoplasmacytic lymphoma, a singular for studying lymphomagenesis because high prevalence mutated MYD88 . observed similar accumulation random lineages from both cohorts unexpectedly found L265P normal precursor mature lymphoma. uncovered genetic...
Abstract We have previously demonstrated that subcutaneously administered ovalbumin (OVA) plus synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs (CpG-ODN) as adjuvant stimulate cellular and humoral immunity promote T helper cell type 1 differentiation in aged mice. The present study assessed the ability of CpG-ODN to induce an OVA-specific immune response after oral immunization young (3-month-old) (18-month-old) BALB/c Oral OVA/CpG-ODN induces a similar...