A5079762316- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Genomics and Chromatin Dynamics
- Acute Myeloid Leukemia Research
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Drug Transport and Resistance Mechanisms
Universidad de Navarra
2020-2023
Wellcome/MRC Cambridge Stem Cell Institute
2020
University of Pittsburgh
2020
Clinica Universidad de Navarra
2020
Abstract Cellular differentiation requires extensive alterations in chromatin structure and function, which is elicited by the coordinated action of transcription factors. By contrast with factors, roles factors have not been systematically characterized. Here, we combine bulk ex vivo single-cell CRISPR screens to characterize role factor families hematopoiesis. We uncover marked lineage specificities for 142 revealing functional diversity among related (i.e. barrier-to-autointegration...
Abstract Background The discovery of functionally relevant KRAS effectors in lung and pancreatic ductal adenocarcinoma (LUAD PDAC) may yield novel molecular targets or mechanisms amenable to inhibition strategies. Phospholipids availability has been appreciated as a mechanism modulate oncogenic potential. Thus, phospholipid transporters play functional role KRAS-driven oncogenesis. Here, we identified systematically studied the transporter PITPNC1 its controlled network LUAD PDAC. Methods...
The COVID-19 pandemic has presented an unprecedent challenge to develop and validate testing tools for urgent disease diagnosis. Current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via molecular diagnostics is woefully inadequate due myriad issues with cross-entity coordination, reagent availability, labor- time-intensive protocols. bottleneck in the workflow from patient sampling diagnosis not identification of viral genetic material (RT-qPCR) but collection, processing,...
The COVID-19 pandemic has presented an unprecedent challenge to develop and validate testing tools for urgent disease diagnosis. Current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via molecular diagnostics is woefully inadequate due myriad issues with cross-entity coordination, reagent availability, labor- time-intensive protocols. bottleneck in the workflow from patient sampling diagnosis not identification of viral genetic material (RT-qPCR) but collection, processing,...
Abstract Interactions between transcription factors (TF) and chromatin (CF) regulate gene expression programmes to determine cellular fate. However, unlike for TF, the exact role of CF in this process is poorly understood. Using haematopoiesis as a model system utilising novel functional CRISPR screens ex vivo , coupled with Perturb-Seq, binding genome-wide accessibility primary murine cells, we assess 550 lineage choice normal maintenance acute myeloid leukaemia (AML). These studies...
Abstract Background Spain is one of the countries most heavily affected by COVID-19 pandemic. As in other such as UK and USA, nursing homes have been an important human reservoir for virus population with highest mortality worldwide. The presence asymptomatic carriers within factors that could provoke new outbreaks during relaxing lockdown measures. Methods We developed a high-throughput protocol RNA extraction patient samples based on silane magnetic beads multi-well plates. sensitivity,...