A5011173949- Glutathione Transferases and Polymorphisms
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Multiple Myeloma Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Estrogen and related hormone effects
- Cancer Treatment and Pharmacology
- Soil Carbon and Nitrogen Dynamics
- Chronic Myeloid Leukemia Treatments
- Prostate Cancer Treatment and Research
- Soil and Water Nutrient Dynamics
- Single-cell and spatial transcriptomics
- Biosimilars and Bioanalytical Methods
- Mesenchymal stem cell research
- RNA modifications and cancer
- Nanowire Synthesis and Applications
- Gene Regulatory Network Analysis
- Anaerobic Digestion and Biogas Production
- Lung Cancer Treatments and Mutations
- Pancreatic function and diabetes
- RNA Research and Splicing
- Bone and Joint Diseases
- T-cell and B-cell Immunology
Universidad de Navarra
2020-2025
Hospital Clínic de Barcelona
2024
Clinica Universidad de Navarra
2024
Centro de Investigación Biomédica en Red de Cáncer
2024
Universitat de Barcelona
2024
Instituto de Salud Carlos III
2024
Navarre Institute of Health Research
2021-2022
Building Engineering and Science Talent
2022
Josep Carreras Leukaemia Research Institute
2020
Institut d'Investigació Biomédica de Bellvitge
2018-2019
Tumors have aberrant proteomes that often do not match their corresponding transcriptome profiles. One possible cause of this discrepancy is the existence RNA modification landscapes in so-called epitranscriptome. Here, we report human glioma cells undergo DNA methylation-associated epigenetic silencing NSUN5, a candidate methyltransferase for 5-methylcytosine. In setting, NSUN5 exhibits tumor-suppressor characteristics vivo models. We also found loss generates an unmethylated status at...
Identification of new markers associated with long-term efficacy in patients treated CAR T cells is a current medical need, particularly diseases such as multiple myeloma. In this study, we address the impact density on functionality BCMA cells. Functional and transcriptional studies demonstrate that high expression construct show an increased tonic signaling up-regulation exhaustion vitro cytotoxicity but decrease vivo BM infiltration. Characterization gene regulatory networks using...
One largely unknown question in cell biology is the discrimination between inconsequential and functional transcriptional events with relevant regulatory functions. Here, we find that oncofetal HMGA2 gene aberrantly reexpressed many tumor types together its antisense transcribed pseudogene RPSAP52. RPSAP52 abundantly present cytoplasm, where it interacts RNA binding protein IGF2BP2/IMP2, facilitating to mRNA targets, promoting their translation by mediating recruitment on polysomes enhancing...
Significance Defects in transfer RNA (tRNA) modifications occur human pathologies such as cancer; however, how these alterations contribute to the disease is poorly understood. One example tumor-specific hypomodification of position 37 tRNA Phe , which was first described 45 y ago, although its cause and consequences have remained unknown. Here we report that due promoter CpG island hypermethylation-associated transcriptional silencing TYW2, a key enzyme synthesis wybutosine derivatives....
Summary An interesting alternative to landfills for disposing of organic residues is their addition soil as composted residues. There little information available about the long‐term benefits following prolonged periods application. After 12 years annual incorporation amendments a vineyard, three characteristics were analysed: mineral content, bacterial community and greenhouse gas ( GHG ) emissions. The (i) pelletized compost PEL made from plant, animal sewage sludge residues, (ii) fraction...
Despite the potential of CAR-T therapies for hematological malignancies, their efficacy in patients with relapse and refractory Acute Myeloid Leukemia has been limited. The aim our study to develop manufacture a cell product that addresses some current limitations. We initially compared phenotype T cells from AML healthy young elderly controls. This analysis showed displayed predominantly effector phenotype, increased expression activation (CD69 HLA-DR) exhaustion markers (PD1 LAG3),...
Hematopoietic stem cells (HSCs) accumulate somatic mutations over time, some conferring a fitness advantage that can lead to clonal hematopoiesis (CH). Mutations in DNMT3A , particularly at hotspot R882, are the most prevalent CH and carry an increased risk of acute myeloid leukemia (AML). Although R882 linked global DNA hypomethylation, mechanisms underlying their selective remain unclear. Here, we show Dnmt3a-R882H mutant HSCs exhibit resilience under inflammatory genotoxic stress. During...
Cardiac fibrosis is mediated by the persistent activity of myofibroblasts, which differentiate from resident cardiac fibroblasts in response to tissue damage and stress signals. The signaling pathways transcription factors regulating fibrotic transformation have been thoroughly studied. In contrast, roles chromatin myofibroblast differentiation their contribution pathogenic remain poorly understood. Here, we combined bulk single-cell CRISPR screens characterize primary fibroblasts. We...
Understanding the mechanisms that drive chimeric antigen receptor (CAR) T cell function and persistence in multiple myeloma (MM) remains a critical challenge for improving therapeutic outcomes. In this study, we applied single-cell multiomics gene regulatory network (GRN) analysis to characterize transcriptional dynamics clonal evolution of BCMA-targeted CAR cells longitudinally collected bone marrow (BM) peripheral blood (PB) samples from MM patients. Our results revealed infiltrating BM...
Cardiac fibrosis is mediated by the persistent activity of myofibroblasts, which differentiate from resident cardiac fibroblasts in response to tissue damage and stress signals. The signaling pathways transcription factors regulating fibrotic transformation have been thoroughly studied. In contrast, roles chromatin myofibroblast differentiation their contribution pathogenic remain poorly understood. Here, we combined bulk single-cell CRISPR screens characterize primary fibroblasts. We...
Abstract Taxanes are standard therapy in clinical practice for metastatic breast cancer; however, primary or acquired chemoresistance a common cause of mortality. Breast cancer patient-derived xenografts (PDX) powerful tools the study biology and drug treatment response. Specific DNA methylation patterns have been associated to different subtypes but its association with remains unstudied. Aiming elucidate docetaxel resistance mechanisms, we performed genome-wide PDX models, including...
Genome-editing strategies, especially CRISPR-Cas9 systems, have substantially increased the efficiency of innovative therapeutic approaches for monogenic diseases such as primary hyperoxalurias (PHs). We previously demonstrated that inhibition glycolate oxidase using systems represents a promising option PH type I (PH1). Here, we extended our work evaluating efficacy liver-specific lactate dehydrogenase (LDH), key enzyme responsible converting glyoxylate to oxalate; this strategy would not...
The endoplasmic reticulum (ER) of cancer cells needs to adapt the enhanced proteotoxic stress associated with accumulation unfolded, misfolded, and transformation-associated proteins. One way by which tumors thrive in context ER is promoting ER-associated degradation (ERAD), although mechanisms are poorly understood. Here, we show that small p97/VCP-interacting protein (SVIP), an endogenous inhibitor ERAD, undergoes DNA hypermethylation–associated silencing tumorigenesis achieve this goal....
Abstract Single-cell RNA-Sequencing has the potential to provide deep biological insights by revealing complex regulatory interactions across diverse cell phenotypes at single-cell resolution. However, current gene network inference methods produce a single per input dataset, limiting their capability uncover relationships related phenotypes. We present SimiC, framework that overcomes this limitation jointly inferring distinct, but related, dynamics phenotype. show SimiC uncovers key missed...
Abstract Single-cell RNA-Sequencing has made it possible to infer high-resolution gene regulatory networks (GRNs), providing deep biological insights by revealing interactions at single-cell resolution. However, current GRN analysis methods produce only a single per input dataset, potentially missing relationships between cells from different phenotypes. To address this issue, we present SimiC, inference method that produces phenotype while imposing similarity constraint forces smooth...