A5054566975- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- FOXO transcription factor regulation
- Cancer-related molecular mechanisms research
- Renal and related cancers
- PARP inhibition in cancer therapy
- RNA Research and Splicing
- Caveolin-1 and cellular processes
- CAR-T cell therapy research
- Plant Disease Resistance and Genetics
- Microbial metabolism and enzyme function
- Cell death mechanisms and regulation
- Signaling Pathways in Disease
- Cancer-related gene regulation
- Virus-based gene therapy research
- Circular RNAs in diseases
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Integrated Circuits and Semiconductor Failure Analysis
- Neuroblastoma Research and Treatments
- Microtubule and mitosis dynamics
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Eosinophilic Disorders and Syndromes
Institut d'Investigació Biomédica de Bellvitge
2014-2020
Instituto de Salud Carlos III
2018-2020
Centro de Investigación Biomédica en Red de Cáncer
2018-2020
St Anna Children's Hospital
2011-2016
St. Anna Children's Cancer Research Institute
2011-2016
University Children's Hospital Zurich
2010-2014
Cancer Research Institute of the Slovak Academy of Sciences
2012
Centro de Investigación del Cáncer
2009
BACKGROUND: Ewing sarcoma is a paradigm of solid tumour -bearing chromosomal translocations resulting in fusion proteins that act as deregulated transcription factors. fuse the EWS gene with an ETS factor, mainly FLI1. Most EWS–FLI1 target genes still remain unknown and many have been identified heterologous model systems. METHODS: We developed stable RNA interference knocking down cell line TC71. Gene expression analyses were performed to study effect on genetic signature identify could...
// José Luis Ordóñez 1,4,* , Ana Teresa Amaral Angel M. Carcaboso 2 David Herrero-Martín 3 María del Carmen García-Macías 4 Vicky Sevillano Diego Alonso Guillem Pascual-Pasto Laura San-Segundo Monica Vila-Ubach Telmo Rodrigues Susana Fraile Cristina Teodosio Agustín Mayo-Iscar 5 Miguel Aracil 6 Carlos Galmarini Oscar Tirado Jaume Mora and Enrique de Álava 1,4 1 Laboratory of Molecular Pathology, Instituto Biomedicina Sevilla (IBiS), Hospital Universitario Virgen Rocio/CSIC/Universidad...
Ewing sarcoma (ES) is the second most common bone malignancy affecting children and young adults with poor prognosis due to high metastasis incidence. Our group previously described that EphA2, a tyrosine kinase receptor, promotes angiogenesis in cells via ligand‐dependent signaling. Now we wanted explore EphA2 ligand‐independent activity, controlled upon phosphorylation at S897 (p‐EphA2 ), as it has been linked several malignancies. By reverse genetic engineering explored phenotypic changes...
One largely unknown question in cell biology is the discrimination between inconsequential and functional transcriptional events with relevant regulatory functions. Here, we find that oncofetal HMGA2 gene aberrantly reexpressed many tumor types together its antisense transcribed pseudogene RPSAP52. RPSAP52 abundantly present cytoplasm, where it interacts RNA binding protein IGF2BP2/IMP2, facilitating to mRNA targets, promoting their translation by mediating recruitment on polysomes enhancing...
// Laura Lagares-Tena 1 , Silvia García-Monclús Roser López-Alemany Olga Almacellas-Rabaiget Juan Huertas-Martínez Miguel Sáinz-Jaspeado Mateo-Lozano 2 Carlos Rodríguez-Galindo 3 Santiago Rello-Varona David Herrero-Martín Oscar M. Tirado Sarcoma Research Group, Institut d’Investigació Biomèdica de Bellvitge-IDIBELL, L’Hospitalet Llobregat, Barcelona, Spain Developmental Tumor Biology Laboratory, Hospital Sant Joan...
Pediatric tumors harbor very low numbers of somatic mutations and therefore offer few targets to improve therapeutic management with targeted drugs. In particular, outcomes remain dismal for patients metastatic alveolar rhabdomyosarcoma (aRMS), where the chimeric transcription factor PAX3/7-FOXO1 has been implicated but problematic target. this report, we addressed challenge by developing a two-armed screen druggable upstream regulatory kinases in pathway. Screening libraries kinome siRNA...
The European Network for Cancer Research in Children and Adolescents (ENCCA) provides an interaction platform stakeholders research care of children with cancer. Among ENCCA objectives is the establishment biology-based prioritization mechanisms selection innovative targets, drugs, prognostic markers validation clinical trials. Specifically sarcomas, there a burning need novel treatment options, since current chemotherapeutic protocols have met their limits. This most obvious metastatic...
// Juan Huertas-Martínez 1 , Santiago Rello-Varona David Herrero-Martín Ignasi Barrau Silvia García-Monclús Miguel Sáinz-Jaspeado Laura Lagares-Tena Yaiza Núñez-Álvarez 5 Mateo-Lozano 2 Jaume Mora Josep Roma 3 Nuria Toran Sebastian Moran 4 Roser López-Alemany Soledad Gallego Manel Esteller A. Peinado Xavier García del Muro and Oscar M. Tirado Sarcoma research group, Molecular Oncology Lab, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain...
Ewing's sarcoma family tumors (ESFT) are characterized by specific chromosomal translocations, which give rise to EWS-ETS chimeric proteins. These aberrant transcription factors the main pathogenic drivers of ESFT. Elucidation influencing expression and/or activity will guide development novel therapeutic agents against this fatal disease.
This report summarizes the results of 3rd Joint ENCCA-WP7, EuroSarc, EEC, PROVABES, and EURAMOS European Bone Sarcoma Network Meeting, which was held at Children's Cancer Research Institute in Vienna, Austria on September 24–25, 2015. The joint bone sarcoma network meetings bring together researchers to present discuss current knowledge biology, genetics, immunology, as well from preclinical investigations clinical trials, generate novel hypotheses for collaborative biological...
// Raphaela Schwentner 1 , David Herrero-Martin 1, 5 Maximilian O Kauer Cornelia N Mutz Anna M Katschnig Grzegorz Sienski 2, 6 Javier Alonso 3 Dave NT Aryee 4 Heinrich Kovar Children´s Cancer Research Institute, St. Kinderkrebsforschung, Vienna 1090, Austria 2 Institute of Molecular Biotechnology the Austrian Academy Sciences, Biocenter Campus, 1030 Vienna, Unidad de Tumores Sólidos Infantiles, Instituto Investigación Enfermedades Raras, ISCIII, Ctra, Majadahonda-Pozuelo Km 28220 Madrid,...
Abstract Oncogenic addiction provides an opportunity to develop new treatment options, especially for childhood cancers. Pediatric tumors contain a lower number of oncogenic mutations compared most adult cancers, suggesting stronger dependency on individual oncogenes, such as chimeric transcription factors that have the ability control multiple pathways. Taking advantage this addiction, targeting becomes powerful strategy therapy translocation positive pediatric like alveolar...
Introduction: Pediatric sarcomas are a heterogeneous group of bone and soft tissue malignancies affecting children young adults. Some these highly aggressive tumors mesenchymal origin harbor characteristic chromosomal translocation. One the most important prognostic factors those diseases is presence metastasis at diagnosis. In that context, we have developed novel orthotopic model, which can be used both for study spontaneous also evaluating therapeutic index in onset metastasis.
Introduction: Rhabdomyosarcoma (RMS) is the most common soft tissue malignancy in childhood and adolescence that originates as a consequence of regulatory disruption growth differentiation muscle precursor cells. Previous results our group deciphered epigenetic profiling RMS, compared to healthy tissue, revealing hypomethylation potential enhancer LOXL2. LOXL2 an amine oxidase catalyzes covalent crosslinking collagen elastin extracellular matrix. Apart from its traditional role, novel...
Abstract Pediatric sarcomas represent a diverse group of rare bone and soft tissue malignancies. Although the molecular mechanisms that propel development these cancers are not well understood, identification tumor-specific translocations in many has provided significant insight into their tumorigenesis. Besides acting as direct modulator transcription, fusion proteins appear to exert its oncogenic functions by epigenetic modifications on transcriptome. Aberrant DNA methylations thought be...
Abstract MicroRNAs (miRNA) serve to fine-tune gene expression and thus play an important regulatory role in tissue specific networks. The identification validation of miRNA target genes a given still poses significant problem since the presence seed sequence 3′UTR mRNA its modulation upon forced ectopic do not reliably predict regulation under physiological conditions. chimeric oncoprotein EWS-FLI1 is driving pathogenic force ES. Recently, we reported on microRNA signature based knockdown...
Abstract MicroRNAs (miRNA) serve to fine-tune gene expression and thus play an important regulatory role in tissue specific networks. The identification validation of miRNA target genes a given still poses significant problem, since the presence seed sequence 3´UTR mRNA its modulation upon forced ectopic do not reliably predict regulation under physiological conditions. Here, we used combination AGO2 pull-down experiments by PAR-CLIP - enrich RISC-associated RNAs RNAseq depletion sponge...
Abstract The identification of microRNA (miR) targets within a given cellular context still poses largely unsolved problem. MiR are predicted based on the presence seed sequences in 3'UTR mRNAs. Target candidate lists filtered assuming mRNA destabilization, and validated by artificially forcing ectopic expression miR analogs, or interfering with antagomirs. However, level amplitude physiological variations is much smaller than experimentally induced perturbations. Crosslinking immuno...
<p>Supplementary Figure Legends. Legend for Supplementary Figures S1-S7.</p>