A5062118868- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Liver physiology and pathology
- Pancreatic function and diabetes
- Biofuel production and bioconversion
- Animal Genetics and Reproduction
- biodegradable polymer synthesis and properties
- Polyamine Metabolism and Applications
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Biological Research and Disease Studies
- bioluminescence and chemiluminescence research
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Organ Transplantation Techniques and Outcomes
- Extracellular vesicles in disease
- Food Allergy and Anaphylaxis Research
- Virus-based gene therapy research
- Mast cells and histamine
- Enzyme Catalysis and Immobilization
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Insect Resistance and Genetics
Instituto de Biologia Experimental e Tecnológica
2022-2024
Universidade Nova de Lisboa
2022-2024
BOKU University
2016-2020
Austrian Centre of Industrial Biotechnology (Austria)
2020
Chinese hamster ovary (CHO) cells produce a large share of today's biopharmaceuticals. Still, the generation satisfactory producer cell lines is tedious undertaking. Recently, it was found that CHO cells, when exposed to new environmental conditions, modify their epigenome, suggesting adapt gene expression pattern handle challenges. The major aim present study employ artificially induced, random changes in DNA-methylation diversify populations and consequently increase finding with improved...
Abstract The insect cell‐baculovirus expression vector system (IC‐BEVS) has shown to be a powerful platform produce complex biopharmaceutical products, such as recombinant proteins and virus‐like particles. More recently, IC‐BEVS also been used an alternative adeno‐associated virus (rAAV). However, little is known about the variability of cell populations potential effect heterogeneity (e.g., stochastic infection process differences in kinetics) on product titer and/or quality. In this...
The insect cell-baculovirus expression vector system (IC-BEVS) has emerged as an alternative time- and cost-efficient production platform for recombinant Adeno-associated virus (AAV) gene therapy. However, a better understanding of the underlying biological mechanisms IC-BEVS is fundamental to further optimize this toward increased product titer quality. Here, Sf9 cells producing AAV through dual baculovirus system, with low multiplicity infection (MOI), was profiled by RNA-seq. An 8-fold...
Adaptive laboratory evolution has been used to improve production of influenza hemagglutinin (HA)-displaying virus-like particles (VLPs) in insect cells. However, little is known about the underlying biological mechanisms promoting higher HA-VLP expression such adapted cell lines. In this article, we present a study gene patterns associated with high-producer High Five cells neutral pH, comparison non-adapted cells, during HA-VLPs. RNA-seq shows decrease amount reads mapping host genomes...
Human diamine oxidase (hDAO) rapidly inactivates histamine by deamination. No pharmacokinetic data are available to better understand its potential as a new therapeutic modality for diseases with excess local and systemic histamine, like anaphylaxis, urticaria or mastocytosis. After intravenous administration of recombinant hDAO rats mice, more than 90% the dose disappeared from plasma pool within 10 min. DAO did not only bind various endothelial epithelial cell lines in vitro, but was also...
The insect cell-baculovirus expression vector system (IC-BEVS) has been widely used to produce recombinant protein at high titers, including complex virus-like particles (VPLs). However, cell-to-cell variability upon infection is yet one of the least understood phenomena in virology, and little known about its impact on production therapeutic proteins. This study aimed dissecting cell population heterogeneity during influenza VLPs IC-BEVS using single-cell RNA-seq (scRNA-seq). High Five was...
Extracellular Vesicles (EV) play a critical role in the regulation of regenerative processes wounded tissues by mediating cell-to-cell communication. Multiple RNA species have been identified EV, although their function still lacks understanding. We previously characterized miRNA content EV secreted over hiPSC-cardiomyocyte differentiation and found distinct expression hiPSC-EV driving its vitro bioactivity. In this work, we investigated piRNA profiles derived from key stages hiPSC-CM...
The insect cell-baculovirus expression vector system (IC-BEVS) has shown to be a powerful platform produce complex biopharmaceutical products, such as recombinant proteins and VLPs. More recently IC-BEVS been also used an alternative adeno-associated virus (AAV). However, little is known about the variability of cell populations potential effect heterogeneity on product titer and/or quality. In this study, transcriptomics analysis Sf9 cells during production AAV using low multiplicity...