A5064725524- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Preterm Birth and Chorioamnionitis
- Polyamine Metabolism and Applications
- Neonatal Respiratory Health Research
- Amino Acid Enzymes and Metabolism
- Gestational Trophoblastic Disease Studies
- Birth, Development, and Health
- Prenatal Screening and Diagnostics
- Maternal and fetal healthcare
- Mast cells and histamine
- Cardiovascular Issues in Pregnancy
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- Immune Cell Function and Interaction
- Immune cells in cancer
- Microbial metabolism and enzyme function
- Pregnancy and Medication Impact
- Reproductive Biology and Fertility
- Food Allergy and Anaphylaxis Research
- Congenital Anomalies and Fetal Surgery
- Cancer-related gene regulation
- Phagocytosis and Immune Regulation
- Kruppel-like factors research
- Hippo pathway signaling and YAP/TAZ
Medical University of Vienna
2017-2023
Linköping University
2018-2021
Genome amplification and cellular senescence are commonly associated with pathological processes. While physiological roles for polyploidization have been described in mouse development, controversy exists over their significance humans. Here, we describe tetraploidization as phenomena of normal human placenta development. During pregnancy, placental extravillous trophoblasts (EVTs) invade the pregnant endometrium, termed decidua, to establish an adapted microenvironment required developing...
lo , have a specific transcriptome, and proliferate in situ d decBAMs are inefficient APCs, induce Tregs, secrete IL-
Abstract Human extravillous trophoblast (EVT) invasion of the pregnant uterus constitutes a pivotal event for establishment maternal-fetal interface. Compromised EVT function manifesting in inadequate arterial remodeling is associated with severe pregnancy disorder early-onset preeclampsia (eoPE). Recent studies suggest that EVTs invade entire uterine vasculature including arteries, veins and lymphatics first trimester pregnancy. We therefore hypothesized EVT-derived factors accumulate...
During pregnancy, the semi-allogeneic nature of foetus requires maternal immune adaption and acquisition tolerance at foetal-maternal interface. Macrophages with regulatory properties T (Treg) cells are central in promoting foetal enriched decidua (the uterine endometrium during pregnancy). Although tissue-resident decidual stromal (DSC) have been implicated functions, it is not known if they able to induce phenotype macrophages T-cells. In this study we report that maternally derived DSC...
During pregnancy, extravillous trophoblasts (EVTs) invade the maternal decidua and remodel local vasculature to establish blood supply for growing fetus. Compromised EVT function has been linked aberrant pregnancy associated with fetal morbidity mortality. However, metabolic features of this invasive trophoblast subtype are largely unknown. Using primary human isolated from first trimester placental tissues, we show that cellular cholesterol homeostasis is differentially regulated in EVTs...
Do high endothelial venules (HEVs) appear in the uterus of healthy and pathological pregnancies?Our study reveals that HEVs are present non-pregnant endometrium decidua parietalis (decP) but decline upon placentation basalis (decB) less abundant decidual tissues from idiopathic, recurrent pregnancy losses (RPLs).RPL is associated with a compromised vascular phenotype.Endometrial (n = 29) first trimester 86, 6-12th week gestation) tissue samples obtained endometrial biopsies or elective...
Background: Excessive plasma histamine concentrations cause symptoms in mast cell activation syndrome, mastocytosis, or anaphylaxis. Anti-histamines are often insufficiently efficacious. Human diamine oxidase (hDAO) can rapidly degrade and therefore represents a promising new treatment strategy for conditions with pathological concentrations. Methods: Positively charged amino acids of the heparin-binding motif hDAO were replaced polar serine threonine residues. Binding to heparin heparan...
Human diamine oxidase (hDAO) rapidly inactivates histamine by deamination. No pharmacokinetic data are available to better understand its potential as a new therapeutic modality for diseases with excess local and systemic histamine, like anaphylaxis, urticaria or mastocytosis. After intravenous administration of recombinant hDAO rats mice, more than 90% the dose disappeared from plasma pool within 10 min. DAO did not only bind various endothelial epithelial cell lines in vitro, but was also...
These authors studied human placental and decidual tissues obtained from elective pregnancy terminations (612 weeks gestation). Placental extravillous trophoblasts (EVTs), the cells that rapidly invade mothers endometrium, undergo an initial stage of genomewide amplification leading to a tetraploid chromosomal state (XXYY or XXXX) followed by cellular senescence. By contrast, androgenic complete hydatidiform moles (CHM) fail show normal
Abstract During human pregnancy, placenta-derived extravillous trophoblasts (EVT) invade the decidua and communicate with maternal immune cells. The can be distinguished into basalis (decB) parietalis (decP), latter being unaffected by placentation. By defining a novel gating strategy, we report accumulation of myeloid cells in decB. We identified basalis-associated macrophage (decBAM) population differential transcriptome secretome when compared to parietalis-associated macrophages...
The human placenta comes in direct contact with maternal cells and blood at two interfaces. syncytiotrophoblast layer is surrounded by the intervillous space, extravillous trophoblasts breach vascular endothelial upon spiral artery remodeling invasion of decidual veins. However, little knowledge exists about EVT-derived secreted factors, which may serve as predictive markers for obstetrical syndromes or shape local environment maternal-fetal interface. Here, we define EVT-associated genes...